Arabidopsis AIP1-1 regulates the organization of apical actin filaments by promoting their turnover in pollen tubes

doi:10.1007/s11427-019-9532-0

	Arabidopsis AIP1-1 regulates the organization of apical actin filaments by promoting their turnover in pollen tubes
	Diao, Min 1,3; Li, Xin 2; Huang, Shanjin 1
	2020
发表期刊	SCIENCE CHINA-LIFE SCIENCES
ISSN	1674-7305
卷号	63 期号:2 页码:239-250
摘要	Apical actin filaments are highly dynamic structures that are crucial for rapid pollen tube growth, but the mechanisms regulating their dynamics and spatial organization remain incompletely understood. We here identify that AtAIP1-1 is important for regulating the turnover and organization of apical actin filaments in pollen tubes. AtAIP1-1 is distributed uniformly in the pollen tube and loss of function of AtAIP1-1 affects the organization of the actin cytoskeleton in the pollen tube. Specifically, actin filaments became disorganized within the apical region of aip1-1 pollen tubes. Consistent with the role of apical actin filaments in spatially restricting vesicles in pollen tubes, the apical region occupied by vesicles becomes enlarged in aip1-1 pollen tubes compared to WT. Using ADF1 as a representative actin-depolymerizing factor, we demonstrate that AtAIP1-1 enhances ADF1-mediated actin depolymerization and filament severing in vitro, although AtAIP1-1 alone does not have an obvious effect on actin assembly and disassembly. The dynamics of apical actin filaments are reduced in aip1-1 pollen tubes compared to WT. Our study suggests that AtAIP1-1 works together with ADF to act as a module in regulating the dynamics of apical actin filaments to facilitate the construction of the unique apical actin structure in the pollen tube.
关键词	pollen tube apical actin structure actin dynamics actin turnover ADF AIP1
学科领域	Biology
DOI	10.1007/s11427-019-9532-0
收录类别	SCI
语种	英语
WOS关键词	DEPOLYMERIZING FACTORS ADF7 ; INTERACTING PROTEIN-1 ; POLARIZED GROWTH ; BINDING PROTEINS ; DYNAMICS ; POLYMERIZATION ; GERMINATION ; HOMOLOG ; COFILIN ; COMPLEX
WOS研究方向	Life Sciences & Biomedicine - Other Topics
WOS记录号	WOS:000511741500006
出版者	SCIENCE PRESS
文献子类	Article
出版地	BEIJING
EISSN	1869-1889
作者邮箱	sjhuang@tsinghua.edu.cn
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21924
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 2.Tsinghua Univ, Sch Life Sci, Ctr Plant Biol, Beijing 100084, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Shanghai Tech Univ, IHuman Inst, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Diao, Min,Li, Xin,Huang, Shanjin. Arabidopsis AIP1-1 regulates the organization of apical actin filaments by promoting their turnover in pollen tubes[J]. SCIENCE CHINA-LIFE SCIENCES,2020,63(2):239-250.
APA	Diao, Min,Li, Xin,&Huang, Shanjin.(2020).Arabidopsis AIP1-1 regulates the organization of apical actin filaments by promoting their turnover in pollen tubes.SCIENCE CHINA-LIFE SCIENCES,63(2),239-250.
MLA	Diao, Min,et al."Arabidopsis AIP1-1 regulates the organization of apical actin filaments by promoting their turnover in pollen tubes".SCIENCE CHINA-LIFE SCIENCES 63.2(2020):239-250.

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