Probing the Proteomics Dark Regions by VAILase Cleavage at Aliphatic Amino Acids

doi:10.1021/acs.analchem.9b05048

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	Probing the Proteomics Dark Regions by VAILase Cleavage at Aliphatic Amino Acids
	Sun, Binwen 1; Liu, Zheyi ; Fang, Zheng 1; Dong, Wei 2; Yu, Yang 3,4; Ye, Mingliang ; Liu, Lin 2; Wang, Hongda 3,4; Wang, Fangjun 1
	2020
发表期刊	ANALYTICAL CHEMISTRY
ISSN	0003-2700
卷号	92 期号:3 页码:2770-2777
摘要	Proteomics emerges from the protein identification to protein functional elucidation, which depends to a large extent on the characterization of protein sequences. However, a large part of proteome sequences remains unannotated due to the limitation in proteolytic digestion by golden standard protease trypsin. Herein, we demonstrated that a cyanobacterial protease VAILase could specifically cleave at the short-chain aliphatic amino acids valine, alanine, leucine, isoleucine and threonine with cleavage specificity about 92% in total for proteomic analysis. The unique features of VAILase cleavage facilitate the characterization of most proteins and exhibit high complementarity to trypsin, and 22% of the covered sequences by VAILase are unique. VAILase can greatly improve the coverages of sequences with abundant aliphatic residues that are usually dark regions in conventional proteomic analysis, such as the transmembrane regions within anion exchanger 1 and photosystem II.
学科领域	Chemistry, Analytical
DOI	10.1021/acs.analchem.9b05048
收录类别	SCI
语种	英语
WOS关键词	INTEGRAL MEMBRANE-PROTEINS ; EVOLVING PHOTOSYSTEM-II ; CRYSTAL-STRUCTURE ; HHOA PROTEASE ; TRYPSIN ; THROUGHPUT ; ACCURACIES ; BAND-3
WOS研究方向	Chemistry
WOS记录号	WOS:000511509400056
出版者	AMER CHEMICAL SOC
文献子类	Article
出版地	WASHINGTON
EISSN	1520-6882
资助机构	National Key R&D Program of China [2016YFF0200504] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [91853101, 21675152] ; Original Innovation Project of CAS [ZDBSLY-SLH032] ; DICP [ZZBS201603]
作者邮箱	wangfj@dicp.ac.cn
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21928
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China 4.Chinese Acad Sci, Changchun Inst Appl Chem, Changchun, Peoples R China 5.Univ Sci & Technol China, Hefei, Peoples R China
推荐引用方式 GB/T 7714	Sun, Binwen,Liu, Zheyi,Fang, Zheng,et al. Probing the Proteomics Dark Regions by VAILase Cleavage at Aliphatic Amino Acids[J]. ANALYTICAL CHEMISTRY,2020,92(3):2770-2777.
APA	Sun, Binwen.,Liu, Zheyi.,Fang, Zheng.,Dong, Wei.,Yu, Yang.,...&Wang, Fangjun.(2020).Probing the Proteomics Dark Regions by VAILase Cleavage at Aliphatic Amino Acids.ANALYTICAL CHEMISTRY,92(3),2770-2777.
MLA	Sun, Binwen,et al."Probing the Proteomics Dark Regions by VAILase Cleavage at Aliphatic Amino Acids".ANALYTICAL CHEMISTRY 92.3(2020):2770-2777.

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