Proteomic variation in Vitis amurensis and V. vinifera buds during cold acclimation

doi:10.1016/j.scienta.2019.109143

	Proteomic variation in Vitis amurensis and V. vinifera buds during cold acclimation
	Masocha, Valerie Farai 1,2; Li, Qingyun 1,3,4; Zhu, Zhenfei 2; Chai, Fengmei ; Sun, Xiaoming ; Wang, Zemin 2,5; Yang, Long 3,4; Wang, Qingfeng 1; Xin, Haiping 1
	2020
发表期刊	SCIENTIA HORTICULTURAE
ISSN	0304-4238
卷号	263
摘要	Amur grape (Vitis amurensis) is a wild grape species with excellent freezing tolerance compared with the widely cultivated common grapevine (V. vinifera). Here, we investigated the effect of cold acclimation (CA) on the proteomes of V. amurensis (cv. Zuoshan-1) and V. vinifera (cv. Jingzaojing) buds to explore the cold-tolerance mechanisms used by these species. The buds were collected in late fall (October) and early winter (December) and subjected to an iTRAQ-based proteomic analysis. A total of 472 and 713 differentially abundant proteins (DAPs) were identified between the two time points in V. amurensis and V. vinifera, respectively. The two species shared 235 DAPs, which were mainly involved in the protein chaperone and metabolic pathways, particularly carbohydrate metabolism. These DAPs represent the general responses to CA in Vitis species. V. amurensis contained less unique DAPs (237) than V. vinifera (478). A functional category analysis indicated that the phenylpropanoid biosynthesis pathway was enriched in V. amurensis. Among the DAPs identified in this pathway, seven upregulated and three downregulated DAPs were present in V. amurensis, while three upregulated and 20 downregulated DAPs were present in V. vinifera. Contrasting patterns were observed between the two species for phenylalanine ammonia-lyase, cinnamoyl-CoA reductase 1, and shikimate O-hydroxycinnamoyl transferase, which accumulated in V. amurensis but decreased in V. vinifera. The qRT-PCR results indicated that the transcriptional changes of 12 genes encoding selected DAPs were all consistent with the changes observed at the protein level. Our work provides new insights into the mechanisms by which cold hardiness is achieved in V. amurensis buds.
关键词	Grapevine Vitis amurensis iTRAQ Cold acclimation Phenylpropanoid biosynthesis pathway
学科领域	Horticulture
DOI	10.1016/j.scienta.2019.109143
收录类别	SCI
语种	英语
WOS关键词	GENE-EXPRESSION ; LOW-TEMPERATURE ; ARABIDOPSIS-THALIANA ; STRESS-RESPONSE ; ABIOTIC STRESS ; PLANT ; ACCUMULATION ; SUGAR ; PERSPECTIVES ; METABOLISM
WOS研究方向	Agriculture
WOS记录号	WOS:000508491600032
出版者	ELSEVIER
文献子类	Article
出版地	AMSTERDAM
EISSN	1879-1018
资助机构	National Key Research and Development Program of China [2018YFD1000300] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31672132] ; Grape Breeding Project of Ningxia [NXNYYZ201502]
作者邮箱	xinhaiping@wbgcas.cn
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21948
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Innovat Acad Seed Design, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan Bot Garden, Wuhan 430074, Peoples R China 2.Chinese Acad Sci, Ctr Econ Bot, Core Bot Gardens, Wuhan 430074, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Shandong Agr Univ, Agr Big Data Res Ctr, Tai An 271018, Shandong, Peoples R China 5.Shandong Agr Univ, Coll Plant Protect, Tai An 271018, Shandong, Peoples R China 6.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing Key Lab Grape Sci & Enol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Masocha, Valerie Farai,Li, Qingyun,Zhu, Zhenfei,et al. Proteomic variation in Vitis amurensis and V. vinifera buds during cold acclimation[J]. SCIENTIA HORTICULTURAE,2020,263.
APA	Masocha, Valerie Farai.,Li, Qingyun.,Zhu, Zhenfei.,Chai, Fengmei.,Sun, Xiaoming.,...&Xin, Haiping.(2020).Proteomic variation in Vitis amurensis and V. vinifera buds during cold acclimation.SCIENTIA HORTICULTURAE,263.
MLA	Masocha, Valerie Farai,et al."Proteomic variation in Vitis amurensis and V. vinifera buds during cold acclimation".SCIENTIA HORTICULTURAE 263(2020).

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