Development of a nornicotine-reduced flue-cured tobacco line via EMS mutagenesis of nicotine N-demethylase genes

doi:10.1080/15592324.2019.1710053

	Development of a nornicotine-reduced flue-cured tobacco line via EMS mutagenesis of nicotine N-demethylase genes
	Song, Zhongbang 1; Sui, Xueyi 1; Li, Meiyun 1; Gao, Yulong 1; Li, Wenzheng 1; Zhao, Lu 1; Li, Feng 2; Yao, Xuefeng 3,4; Liu, Chunming3,5 ; Wang, Bingwu 1
	2020
发表期刊	PLANT SIGNALING & BEHAVIOR
ISSN	1559-2316
卷号	15 期号:2
摘要	Substantial progress had been made in reducing nornicotine accumulation in burley tobacco, as nornicotine is a precursor of the carcinogen N-nitrosonornicotine (NNN). Three members of the CYP82E2 family encoding nicotine N-demethylase (NND) have been reported to be responsible for the majority of nicotine demethylation that forms nornicotine in burley tobacco. We had obtained a nonsense mutant of each NND member in flue-cured tobacco from an ethyl methanesulfonate (EMS)-mutagenized population. In this study, we developed dCAPS markers for each nonsense mutation. Using marker-assisted selection, NND mutants were crossed with each other to generate a triple mutant GP449. In line with previous reports, the triple knockout caused significantly decreased levels of nornicotine and NNN in flue-cured tobacco. With the decreased nornicotine, the nicotine level was expected to accumulate. However, the nicotine level in GP449 was significantly decreased to 72.80% of wild type. Realtime RT-PCR analysis showed that the nicotine reduction was correlated with inhibited expression of nicotine biosynthetic pathway genes. The triple mutant and dCAPS markers can be utilized to develop new flue-cured tobacco varieties with lower levels of nornicotine and NNN.
关键词	Nornicotine NNN Nicotine demethylation dCAPS marker Tobacco
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1080/15592324.2019.1710053
收录类别	SCI
语种	英语
WOS关键词	ONE-CARBON METABOLISM ; METHYLPUTRESCINE OXIDASE ; TABACUM ; NITROSAMINES ; CONVERSION ; EXPRESSION
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS记录号	WOS:000505440600001
出版者	TAYLOR & FRANCIS INC
文献子类	Article
出版地	PHILADELPHIA
EISSN	1559-2324
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31560080] ; China Tobacco Company ; Yunnan Tobacco Company [110201801027, JY-04, 2016YN25, 2017YN03, 2018530000241001]
作者邮箱	bwwang76@hotmail.com
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21961
专题	中科院植物分子生理学重点实验室
作者单位	1.Yunnan Acad Tobacco Agr Sci, Kunming 650021, Yunnan, Peoples R China 2.Natl Ctr Tobacco Gene Engn, Kunming, Yunnan, Peoples R China 3.Zhengzhou Tobacco Res Inst CNTC, China Tobacco Gene Res Ctr, Zhengzhou, Henan, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China 6.Chinese Acad Agr Sci, Inst Crop Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Song, Zhongbang,Sui, Xueyi,Li, Meiyun,et al. Development of a nornicotine-reduced flue-cured tobacco line via EMS mutagenesis of nicotine N-demethylase genes[J]. PLANT SIGNALING & BEHAVIOR,2020,15(2).
APA	Song, Zhongbang.,Sui, Xueyi.,Li, Meiyun.,Gao, Yulong.,Li, Wenzheng.,...&Wang, Bingwu.(2020).Development of a nornicotine-reduced flue-cured tobacco line via EMS mutagenesis of nicotine N-demethylase genes.PLANT SIGNALING & BEHAVIOR,15(2).
MLA	Song, Zhongbang,et al."Development of a nornicotine-reduced flue-cured tobacco line via EMS mutagenesis of nicotine N-demethylase genes".PLANT SIGNALING & BEHAVIOR 15.2(2020).

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