PPR-SMR protein SOT1 has RNA endonuclease activity

doi:10.3389/fpls.2017.02134

IB-CAS > 中科院光生物学重点实验室

	PPR-SMR protein SOT1 has RNA endonuclease activity
	Zhou, Wen 1; Lu, Qingtao; Li, Qingwei 1; Wang, Lei1 ; Ding, Shunhua ; Zhang, Aihong ; Wen, Xiaogang ; Zhang, Lixin; Lu, Congming 2
	2017
发表期刊	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN	0027-8424
卷号	114 期号:8 页码:E1554-E1563
摘要	Numerous attempts have been made to identify and engineer sequence-specific RNA endonucleases, as these would allow for efficient RNA manipulation. However, no natural RNA endonuclease that recognizes RNA in a sequence-specific manner has been described to date. Here, we report that SUPPRESSOR OF THYLAKOID FORMATION 1 (SOT1), an Arabidopsis pentatricopeptide repeat (PPR) protein with a small MutS-related (SMR) domain, has RNA endonuclease activity. We show that the SMR moiety of SOT1 performs the endonucleolytic maturation of 23S and 4.5S rRNA through the PPR domain, specifically recognizing a 13-nucleotide RNA sequence in the 5' end of the chloroplast 23S-4.5S rRNA precursor. In addition, we successfully engineered the SOT1 protein with altered PPR motifs to recognize and cleave a predicted RNA substrate. Our findings point to SOT1 as an exciting tool for RNA manipulation.
关键词	photosynthesis PPR-SMR protein RNA endonuclease rRNA biogenesis
学科领域	Plant Sciences
DOI	10.3389/fpls.2017.02134
收录类别	SCI
语种	英语
WOS关键词	PENTATRICOPEPTIDE REPEAT PROTEIN ; SINGLE-STRANDED RNA ; STRUCTURAL BASIS ; MODULAR RECOGNITION ; CRYSTAL-STRUCTURE ; MINI-III ; DOMAIN ; MUTS2 ; TRANSLATION ; MATURATION
WOS记录号	WOS:000417788900003
出版者	NATL ACAD SCIENCES
文献子类	Article
出版地	WASHINGTON
资助机构	Key Research Plan of Frontier Sciences of the Chinese Academy of Sciences [QYZDJ-SSWSMC003] ; State Key Basic Research and Development Plan of ChinaNational Basic Research Program of China [2015CB150105] ; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDB17030100]
作者邮箱	lucm@ibcas.ac.cn
作品OA属性	Green Published, gold
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21969
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 3.Natl Ctr Plant Gene Res, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Wen,Lu, Qingtao,Li, Qingwei,et al. PPR-SMR protein SOT1 has RNA endonuclease activity[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2017,114(8):E1554-E1563.
APA	Zhou, Wen.,Lu, Qingtao.,Li, Qingwei.,Wang, Lei.,Ding, Shunhua.,...&Lu, Congming.(2017).PPR-SMR protein SOT1 has RNA endonuclease activity.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,114(8),E1554-E1563.
MLA	Zhou, Wen,et al."PPR-SMR protein SOT1 has RNA endonuclease activity".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 114.8(2017):E1554-E1563.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Zhang-2017-Spatial P（2423KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文