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PPR-SMR protein SOT1 has RNA endonuclease activity
Zhou, Wen1; Lu, Qingtao; Li, Qingwei1; Wang, Lei1; Ding, Shunhua; Zhang, Aihong; Wen, Xiaogang; Zhang, Lixin; Lu, Congming2
2017
发表期刊PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN0027-8424
卷号114期号:8页码:E1554-E1563
摘要Numerous attempts have been made to identify and engineer sequence-specific RNA endonucleases, as these would allow for efficient RNA manipulation. However, no natural RNA endonuclease that recognizes RNA in a sequence-specific manner has been described to date. Here, we report that SUPPRESSOR OF THYLAKOID FORMATION 1 (SOT1), an Arabidopsis pentatricopeptide repeat (PPR) protein with a small MutS-related (SMR) domain, has RNA endonuclease activity. We show that the SMR moiety of SOT1 performs the endonucleolytic maturation of 23S and 4.5S rRNA through the PPR domain, specifically recognizing a 13-nucleotide RNA sequence in the 5' end of the chloroplast 23S-4.5S rRNA precursor. In addition, we successfully engineered the SOT1 protein with altered PPR motifs to recognize and cleave a predicted RNA substrate. Our findings point to SOT1 as an exciting tool for RNA manipulation.
关键词photosynthesis PPR-SMR protein RNA endonuclease rRNA biogenesis
学科领域Plant Sciences
DOI10.3389/fpls.2017.02134
收录类别SCI
语种英语
WOS关键词PENTATRICOPEPTIDE REPEAT PROTEIN ; SINGLE-STRANDED RNA ; STRUCTURAL BASIS ; MODULAR RECOGNITION ; CRYSTAL-STRUCTURE ; MINI-III ; DOMAIN ; MUTS2 ; TRANSLATION ; MATURATION
WOS记录号WOS:000417788900003
出版者NATL ACAD SCIENCES
文献子类Article
出版地WASHINGTON
资助机构Key Research Plan of Frontier Sciences of the Chinese Academy of Sciences [QYZDJ-SSWSMC003] ; State Key Basic Research and Development Plan of ChinaNational Basic Research Program of China [2015CB150105] ; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDB17030100]
作者邮箱lucm@ibcas.ac.cn
作品OA属性Green Published, gold
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/21969
专题中科院光生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
3.Natl Ctr Plant Gene Res, Beijing 100093, Peoples R China
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GB/T 7714
Zhou, Wen,Lu, Qingtao,Li, Qingwei,et al. PPR-SMR protein SOT1 has RNA endonuclease activity[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2017,114(8):E1554-E1563.
APA Zhou, Wen.,Lu, Qingtao.,Li, Qingwei.,Wang, Lei.,Ding, Shunhua.,...&Lu, Congming.(2017).PPR-SMR protein SOT1 has RNA endonuclease activity.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,114(8),E1554-E1563.
MLA Zhou, Wen,et al."PPR-SMR protein SOT1 has RNA endonuclease activity".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 114.8(2017):E1554-E1563.
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