Knowledge Management System Of Institute Of Botany,CAS
| The V-ATPase subunit A is essential for salt tolerance through participating in vacuolar Na+ compartmentalization in Salicornia europaea | |
Lv, Sulian; Jiang, Ping; Tai, Fang; Wang, Duoliya; Feng, Juanjuan; Fan, Pengxiang; Bao, Hexigeduleng; Li, Yinxin
| |
| 2017 | |
| Source Publication | PLANTA
 |
| ISSN | 0032-0935 |
| Volume | 246Issue:6Pages:1177-1187 |
| Abstract | The V-ATPase subunit A participates in vacuolar Na (+) compartmentalization in Salicornia europaea regulating V-ATPase and V-PPase activities. Na+ sequestration into the vacuole is an efficient strategy in response to salinity in many halophytes. However, it is not yet fully understood how this process is achieved. Particularly, the role of vacuolar H+-ATPase (V-ATPase) in this process is controversial. Our previous proteomic investigation in the euhalophyte Salicornia europaea L. found a significant increase of the abundance of V-ATPase subunit A under salinity. Here, the gene encoding this subunit named SeVHA-A was characterized, and its role in salt tolerance was demonstrated by RNAi directed downregulation in suspension-cultured cells of S. europaea. The transcripts of genes encoding vacuolar H+-PPase (V-PPase) and vacuolar Na+/H+ antiporter (SeNHX1) also decreased significantly in the RNAi cells. Knockdown of SeVHA-A resulted in a reduction in both V-ATPase and vacuolar H+-PPase (V-PPase) activities. Accordingly, the SeVHA-A-RNAi cells showed increased vacuolar pH and decreased cell viability under different NaCl concentrations. Further Na+ staining showed the reduced vacuolar Na+ sequestration in RNAi cells. Taken together, our results evidenced that SeVHA-A participates in vacuolar Na+ sequestration regulating V-ATPase and V-PPase activities and thereby vacuolar pH in S. europaea. The possible mechanisms underlying the reduction of vacuolar V-PPase activity in SeVHA-A-RNAi cells were also discussed. |
| Keyword | Halophyte Salinity Sodium sequestration Transformation system V-PPase |
| Subject Area | Environmental Sciences & Ecology ; Geology |
| DOI | 10.5194/bg-14-3947-2017 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | ROOT PLASMA-MEMBRANE ; H+-PPASE GENE ; SALINITY TOLERANCE ; ENDOPLASMIC-RETICULUM ; PLANT-GROWTH ; ARABIDOPSIS ; TONOPLAST ; PYROPHOSPHATASE ; OVEREXPRESSION ; TRANSPORT |
| WOS ID | WOS:000410559400001 |
| Publisher | SPRINGER |
| Subtype | Article |
| Publication Place | NEW YORK |
| EISSN | 1432-2048 |
| Funding Organization | National Research and Development Project of Transgenic Crops of China [2016ZX08009003-002] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270421] |
| Corresponding Author Email | yxli@ibcas.ac.cn |
| OA | Green Submitted, gold |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/21990 |
| Collection | 中科院植物分子生理学重点实验室 |
| Affiliation | Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China |
| Recommended Citation GB/T 7714 | Lv, Sulian,Jiang, Ping,Tai, Fang,et al. The V-ATPase subunit A is essential for salt tolerance through participating in vacuolar Na+ compartmentalization in Salicornia europaea[J]. PLANTA,2017,246(6):1177-1187. |
| APA | Lv, Sulian.,Jiang, Ping.,Tai, Fang.,Wang, Duoliya.,Feng, Juanjuan.,...&Li, Yinxin.(2017).The V-ATPase subunit A is essential for salt tolerance through participating in vacuolar Na+ compartmentalization in Salicornia europaea.PLANTA,246(6),1177-1187. |
| MLA | Lv, Sulian,et al."The V-ATPase subunit A is essential for salt tolerance through participating in vacuolar Na+ compartmentalization in Salicornia europaea".PLANTA 246.6(2017):1177-1187. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Song-2017-Initial sh（3818KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment