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The V-ATPase subunit A is essential for salt tolerance through participating in vacuolar Na+ compartmentalization in Salicornia europaea
Lv, Sulian; Jiang, Ping; Tai, Fang; Wang, Duoliya; Feng, Juanjuan; Fan, Pengxiang; Bao, Hexigeduleng; Li, Yinxin
2017
发表期刊PLANTA
ISSN0032-0935
卷号246期号:6页码:1177-1187
摘要The V-ATPase subunit A participates in vacuolar Na (+) compartmentalization in Salicornia europaea regulating V-ATPase and V-PPase activities. Na+ sequestration into the vacuole is an efficient strategy in response to salinity in many halophytes. However, it is not yet fully understood how this process is achieved. Particularly, the role of vacuolar H+-ATPase (V-ATPase) in this process is controversial. Our previous proteomic investigation in the euhalophyte Salicornia europaea L. found a significant increase of the abundance of V-ATPase subunit A under salinity. Here, the gene encoding this subunit named SeVHA-A was characterized, and its role in salt tolerance was demonstrated by RNAi directed downregulation in suspension-cultured cells of S. europaea. The transcripts of genes encoding vacuolar H+-PPase (V-PPase) and vacuolar Na+/H+ antiporter (SeNHX1) also decreased significantly in the RNAi cells. Knockdown of SeVHA-A resulted in a reduction in both V-ATPase and vacuolar H+-PPase (V-PPase) activities. Accordingly, the SeVHA-A-RNAi cells showed increased vacuolar pH and decreased cell viability under different NaCl concentrations. Further Na+ staining showed the reduced vacuolar Na+ sequestration in RNAi cells. Taken together, our results evidenced that SeVHA-A participates in vacuolar Na+ sequestration regulating V-ATPase and V-PPase activities and thereby vacuolar pH in S. europaea. The possible mechanisms underlying the reduction of vacuolar V-PPase activity in SeVHA-A-RNAi cells were also discussed.
关键词Halophyte Salinity Sodium sequestration Transformation system V-PPase
学科领域Environmental Sciences & Ecology ; Geology
DOI10.5194/bg-14-3947-2017
收录类别SCI
语种英语
WOS关键词ROOT PLASMA-MEMBRANE ; H+-PPASE GENE ; SALINITY TOLERANCE ; ENDOPLASMIC-RETICULUM ; PLANT-GROWTH ; ARABIDOPSIS ; TONOPLAST ; PYROPHOSPHATASE ; OVEREXPRESSION ; TRANSPORT
WOS记录号WOS:000410559400001
出版者SPRINGER
文献子类Article
出版地NEW YORK
EISSN1432-2048
资助机构National Research and Development Project of Transgenic Crops of China [2016ZX08009003-002] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270421]
作者邮箱yxli@ibcas.ac.cn
作品OA属性Green Submitted, gold
引用统计
被引频次:30[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/21990
专题中科院植物分子生理学重点实验室
作者单位Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
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GB/T 7714
Lv, Sulian,Jiang, Ping,Tai, Fang,et al. The V-ATPase subunit A is essential for salt tolerance through participating in vacuolar Na+ compartmentalization in Salicornia europaea[J]. PLANTA,2017,246(6):1177-1187.
APA Lv, Sulian.,Jiang, Ping.,Tai, Fang.,Wang, Duoliya.,Feng, Juanjuan.,...&Li, Yinxin.(2017).The V-ATPase subunit A is essential for salt tolerance through participating in vacuolar Na+ compartmentalization in Salicornia europaea.PLANTA,246(6),1177-1187.
MLA Lv, Sulian,et al."The V-ATPase subunit A is essential for salt tolerance through participating in vacuolar Na+ compartmentalization in Salicornia europaea".PLANTA 246.6(2017):1177-1187.
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