Cytological and Transcriptomic Analyses Reveal Important Roles of CLE19 in Pollen Exine Formation

doi:10.1016/j.ecolmodel.2017.07.014

	Cytological and Transcriptomic Analyses Reveal Important Roles of CLE19 in Pollen Exine Formation
	Wang, Shuangshuang 1,2; Lu, Jianan 1,2; Song, Xiu-Fen ; Ren, Shi-Chao ; You, Chenjiang 1,2; Xu, Jie 4; Liu, Chun-Ming; Ma, Hong 1,2; Chang, Fang 1,2
	2017
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
卷号	175 期号:3 页码:1186-1202
摘要	The CLAVATA3/ESR-RELATED (CLE) peptide signals are required for cell-cell communication in several plant growth and developmental processes. However, little is known regarding the possible functions of the CLEs in the anther. Here, we show that a T-DNA insertional mutant, and dominant-negative (DN) and overexpression (OX) transgenic plants of the CLE19 gene, exhibited significantly reduced anther size and pollen grain number and abnormal pollen wall formation in Arabidopsis (Arabidopsis thaliana). Interestingly, the DN-CLE19 pollen grains showed a more extensively covered surface, but CLE19-OX pollen exine exhibited clearly missing connections in the network and lacked separation between areas that normally form the lacunae. With a combination of cell biological, genetic, and transcriptomic analyses on cle19, DN-CLE19, and CLE19-OX plants, we demonstrated that CLE19-OX plants produced highly vacuolated and swollen aborted microspores (ams)-like tapetal cells, lacked lipidic tapetosomes and elaioplasts, and had abnormal pollen primexine without obvious accumulation of sporopollenin precursors. Moreover, CLE19 is important for the normal expression of more than 1,000 genes, including the transcription factor gene AMS, 280 AMS-downstream genes, and other genes involved in pollen coat and pollen exine formation, lipid metabolism, pollen germination, and hormone metabolism. In addition, the DN-CLE19(+/+) ams(-/-) plants exhibited the ams anther phenotype and ams(+/-) partially suppressed the DN-CLE19 transgene-induced pollen exine defects. These findings demonstrate that the proper amount of CLE19 signal is essential for the normal expression of AMS and its downstream gene networks in the regulation of anther development and pollen exine formation.
学科领域	Environmental Sciences & Ecology
DOI	10.1016/j.ecolmodel.2017.07.014
收录类别	SCI
语种	英语
WOS关键词	RECEPTOR-LIKE KINASES ; EARLY ANTHER DEVELOPMENT ; ABORTED-MICROSPORES AMS ; CELL FATE DETERMINATION ; ARABIDOPSIS-THALIANA ; TAPETUM DEVELOPMENT ; GENE ENCODES ; REPRODUCTIVE DEVELOPMENT ; ENDOSPERM DEVELOPMENT ; PROTEIN-KINASE
WOS记录号	WOS:000411771800035
出版者	AMER SOC PLANT BIOLOGISTS
文献子类	Article
出版地	ROCKVILLE
EISSN	1532-2548
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31130006, 31470282, 31670316, 31370029] ; State Key Laboratory of Genetic Engineering at Fudan University
作者邮箱	fangchang@fudan.edu.cn
作品OA属性	Green Accepted
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21993
专题	中科院植物分子生理学重点实验室
作者单位	1.Fudan Univ, State Key Lab Genet Engn, Shanghai 200438, Peoples R China 2.Fudan Univ, Collaborat Innovat Ctr Genet & Dev, Minist Educ, Key Lab Biodivers Sci & Ecol Engn, Shanghai 200438, Peoples R China 3.Fudan Univ, Inst Biodivers Sci, Inst Plant Biol, Sch Life Sci, Shanghai 200438, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 5.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Collaborat Innovat Ctr Genet & Dev, Shanghai 200240, Peoples R China 6.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Wang, Shuangshuang,Lu, Jianan,Song, Xiu-Fen,et al. Cytological and Transcriptomic Analyses Reveal Important Roles of CLE19 in Pollen Exine Formation[J]. PLANT PHYSIOLOGY,2017,175(3):1186-1202.
APA	Wang, Shuangshuang.,Lu, Jianan.,Song, Xiu-Fen.,Ren, Shi-Chao.,You, Chenjiang.,...&Chang, Fang.(2017).Cytological and Transcriptomic Analyses Reveal Important Roles of CLE19 in Pollen Exine Formation.PLANT PHYSIOLOGY,175(3),1186-1202.
MLA	Wang, Shuangshuang,et al."Cytological and Transcriptomic Analyses Reveal Important Roles of CLE19 in Pollen Exine Formation".PLANT PHYSIOLOGY 175.3(2017):1186-1202.

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