Possible involvement of phosphoenolpyruvate carboxylase and NAD-malic enzyme in response to drought stress. A case study: a succulent nature of the C-4-NAD-ME type desert plant, Salsola lanata (Chenopodiaceae)

doi:10.1093/jpe/rtw090

	Possible involvement of phosphoenolpyruvate carboxylase and NAD-malic enzyme in response to drought stress. A case study: a succulent nature of the C-4-NAD-ME type desert plant, Salsola lanata (Chenopodiaceae)
	Wen, Zhibin 1; Zhang, Mingli 1
	2017
发表期刊	FUNCTIONAL PLANT BIOLOGY
ISSN	1445-4408
卷号	44 期号:12 页码:1219-1228
摘要	The co-ordination between the primary carboxylating enzyme phosphoenolpyruvate carboxylase (PEPC) and the further decarboxylating enzymes is crucial to the efficiency of the CO2-concentrating mechanism in C-4 plants, and investigations on more types of C-4 plants are needed to fully understand their adaptation mechanisms. In this study we investigated the effect of drought on carboxylating enzyme PEPC, and the further decarboxylating NAD-malic enzyme (NAD-ME) of Salsola lanata Pall. (Chenopodiaceae) - an annual succulent C-4-NAD-ME subtype desert plant. We investigated enzyme activity at the transcriptional level with real-time quantitative PCR and at the translational level by immunochemical methods, and compared S. lanata with other forms of studied C-4 plants under drought stress. Results showed that only severe stress limited PEPC enzyme activity (at pH 8.0) of S. lanata significantly. Considering that PEPC enzyme activity (at pH 8.0) was not significantly affected by phosphorylation, the decrease of PEPC enzyme activity (at pH 8.0) of S. lanata under severe stress may be related with decreased PEPC mRNA. The suggestion of increased phosphorylation of the PEPC enzyme in plants under moderate stress was supported by the ratio of PEPC enzyme activity at pH 7.3/8.0, as PEPC enzyme is inhibited by L-malate and the evidence of the 50% inhibiting concentration of L-malate. NAD-ME activity decreased significantly under moderate and severe stress, and coincided with a change of leaf water content rather than the amount of alpha-NAD-ME mRNA and protein. Leaf dehydration may cause the decrease of NAD-ME activity under water stress. Compared with other C-4 plants, the activities of PEPC and NAD-ME of S. lanata under drought stress showed distinct features.
关键词	C-4 photosynthesis drought stress phosphoenolpyruvate carboxylase
学科领域	Plant Sciences ; Environmental Sciences & Ecology ; Forestry
DOI	10.1093/jpe/rtw090
收录类别	SCI
语种	英语
WOS关键词	DIFFERENT METABOLIC SUBTYPES ; 3 C-4 GRASSES ; WATER-STRESS ; REGULATORY PHOSPHORYLATION ; PHOTOSYNTHETIC RESPONSES ; SIGNAL-TRANSDUCTION ; EXPRESSION ; CARBON ; IDENTIFICATION ; DIVERSITY
WOS记录号	WOS:000417977800011
出版者	CSIRO PUBLISHING
文献子类	Article
出版地	CLAYTON
EISSN	1445-4416
资助机构	National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31670339, 31300217] ; CAS West Light Foundation [XBBS201209]
作者邮箱	zhangml@ibcas.ac.cn
作品OA属性	Bronze
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21998
专题	系统与进化植物学国家重点实验室
作者单位	1.Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Key Lab Biogeog & Bioresource Arid Land, CN-830011 Urumqi, Peoples R China 2.Chinese Acad Sci, Inst Bot, CN-100093 Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wen, Zhibin,Zhang, Mingli. Possible involvement of phosphoenolpyruvate carboxylase and NAD-malic enzyme in response to drought stress. A case study: a succulent nature of the C-4-NAD-ME type desert plant, Salsola lanata (Chenopodiaceae)[J]. FUNCTIONAL PLANT BIOLOGY,2017,44(12):1219-1228.
APA	Wen, Zhibin,&Zhang, Mingli.(2017).Possible involvement of phosphoenolpyruvate carboxylase and NAD-malic enzyme in response to drought stress. A case study: a succulent nature of the C-4-NAD-ME type desert plant, Salsola lanata (Chenopodiaceae).FUNCTIONAL PLANT BIOLOGY,44(12),1219-1228.
MLA	Wen, Zhibin,et al."Possible involvement of phosphoenolpyruvate carboxylase and NAD-malic enzyme in response to drought stress. A case study: a succulent nature of the C-4-NAD-ME type desert plant, Salsola lanata (Chenopodiaceae)".FUNCTIONAL PLANT BIOLOGY 44.12(2017):1219-1228.

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