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Establishment of a gene function analysis system for the euhalophyte Salicornia europaea L. | |
Tai, Fang1; Lv, Sulian; Jiang, Ping; Wang, Jinhui1; Feng, Juanjuan; Li, Yinxin | |
2017 | |
发表期刊 | PLANT CELL REPORTS |
ISSN | 0721-7714 |
卷号 | 36期号:8页码:1251-1261 |
摘要 | The exploration of salt-tolerant genes from halophyte has seriously been limited by the lack of self-dependent transformation system. Here, an Agrobacterium tumefaciens-mediated in vitro cell transformation system of euhalophyte Salicornia europaea L. was developed. Calli derived from hypocotyl of S. europaea were co-cultured for 3 days with Agrobacterium at OD600 ranging from 1.0 to 1.5 and then selected with 25 mg/L hygromycin (Hyg). The transformed cells were identified from Hyg positive calli by GUS assay and qRT-PCR, and the transformation efficiency was up to 74.4%. The practicality of this system was further tested via genetic manipulation of S. europaea Na+/H+ antiporter 1 (SeNHX1) gene by creating the overexpressing, silencing, and empty vector cells. Survival ratio and Na+ distribution under salt treatment showed obvious differences in SeNHX1-overexpressing, -silencing, and empty vector cells, indicating the feasibility of this system to analyze gene function. This investigation is enlightening for studies in other non-model plants lacking of self-dependent transformation system. |
关键词 | Salicornia europaea L. Suspension cells In vitro Agrobacterium-mediated transformation SeNHX1 |
学科领域 | Environmental Sciences & Ecology ; Geology |
DOI | 10.1002/2017JG003991 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | COMPARATIVE PROTEOMIC ANALYSIS ; IN-VITRO PLANTLET ; SALT TOLERANCE ; MESEMBRYANTHEMUM-CRYSTALLINUM ; KOSTELETZKYA-VIRGINICA ; CALLUS ; TOBACCO ; CULTURE ; NACL ; TRANSFORMATION |
WOS记录号 | WOS:000414961500002 |
出版者 | SPRINGER |
文献子类 | Article |
出版地 | NEW YORK |
EISSN | 1432-203X |
资助机构 | National Research and Development Project of Transgenic Crops of China [2016ZX08009-003-002] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270421] |
作者邮箱 | yxli@ibcas.ac.cn |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/22002 |
专题 | 中科院植物分子生理学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Key Lab Plant Mol Physiol, Inst Bot, 20 Nanxincun, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Tai, Fang,Lv, Sulian,Jiang, Ping,et al. Establishment of a gene function analysis system for the euhalophyte Salicornia europaea L.[J]. PLANT CELL REPORTS,2017,36(8):1251-1261. |
APA | Tai, Fang,Lv, Sulian,Jiang, Ping,Wang, Jinhui,Feng, Juanjuan,&Li, Yinxin.(2017).Establishment of a gene function analysis system for the euhalophyte Salicornia europaea L..PLANT CELL REPORTS,36(8),1251-1261. |
MLA | Tai, Fang,et al."Establishment of a gene function analysis system for the euhalophyte Salicornia europaea L.".PLANT CELL REPORTS 36.8(2017):1251-1261. |
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