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Isolation and Characterization of Microsatellite Markers for Viola websteri (Violaceae) and Cross-Species Amplification within the Genus Viola
Kang, Jong-Soo; Zhang, Xianchun1; Kwak, Myounghai
2017
发表期刊PLANT MOLECULAR BIOLOGY REPORTER
ISSN0735-9640
卷号35期号:1页码:145-153
摘要We isolated and characterized microsatellite loci in Viola websteri (Violaceae), an endangered species from Korea and endemic to Northeast Asia. A total of 27 microsatellite loci were developed and tested in Korean and Chinese populations. The number of alleles per locus varied from two to eight. The observed and expected heterozygosities within two populations were 0.000 to 1.000 and 0.080 to 0.816, respectively. Korean and Chinese populations were clearly distinguished by the private alleles from 16 loci. A total of 21 loci out of the 27 developed loci were successfully cross-amplified in 39 other Viola species. We believe that these microsatellite loci will be useful for future studies on genetic diversity and population structure of V. websteri, as well as other Viola species.
关键词Viola websteri Viola Microsatellites Cross-amplification Transferability
学科领域Plant Sciences
DOI10.1016/j.jplph.2017.03.009
收录类别SCI
语种英语
WOS关键词POPULATION GENETIC CONSEQUENCES ; SSR MARKERS ; TRANSFERABILITY ; CONSERVATION ; DIVERSITY ; MANAGEMENT ; ERRORS ; LOCI
WOS记录号WOS:000404884100013
出版者SPRINGER
文献子类Article
出版地NEW YORK
EISSN1572-9818
资助机构National Institute of Biological Resources (NIBR) - Ministry of Environment (MOE) of the Republic of Korea [NIBR201503102]
作者邮箱mhkwak1@korea.kr
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/22023
专题系统与进化植物学国家重点实验室
作者单位1.Natl Inst Biol Resources, Plant Resources Div, Incheon 404708, South Korea
2.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China
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Kang, Jong-Soo,Zhang, Xianchun,Kwak, Myounghai. Isolation and Characterization of Microsatellite Markers for Viola websteri (Violaceae) and Cross-Species Amplification within the Genus Viola[J]. PLANT MOLECULAR BIOLOGY REPORTER,2017,35(1):145-153.
APA Kang, Jong-Soo,Zhang, Xianchun,&Kwak, Myounghai.(2017).Isolation and Characterization of Microsatellite Markers for Viola websteri (Violaceae) and Cross-Species Amplification within the Genus Viola.PLANT MOLECULAR BIOLOGY REPORTER,35(1),145-153.
MLA Kang, Jong-Soo,et al."Isolation and Characterization of Microsatellite Markers for Viola websteri (Violaceae) and Cross-Species Amplification within the Genus Viola".PLANT MOLECULAR BIOLOGY REPORTER 35.1(2017):145-153.
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