Overexpression of PSK1, a SKP1-like gene homologue, from Paeonia suffruticosa, confers salinity tolerance in Arabidopsis

doi:10.3389/fpls.2017.01047

	Overexpression of PSK1, a SKP1-like gene homologue, from Paeonia suffruticosa, confers salinity tolerance in Arabidopsis
	Hao, Qing 2; Ren, Hongxu ; Zhu, Jin 1; Wang, Liangsheng; Huang, Shouchen 2; Liu, Zheng'an ; Gao, Zhimin 3; Shu, Qingyan
	2017
发表期刊	PLANT CELL REPORTS
ISSN	0721-7714
卷号	36 期号:1 页码:151-162
摘要	Our study is the first to demonstrate that PSK1 , a SKP1 -like gene homologue, is involved in salinity tolerance. Our functional characterization of PSK1 provides new insights into tree peony development. A homologous gene of S-phase kinase-associated protein1 (SKP1) was cloned from tree peony (Paeonia suffruticosa) and denoted as PSK1. The 462-bp open reading frame of PSK1 was predicted to encode a protein comprising 153 amino acids, with a molecular mass of 17 kDa. The full-length gene was 1,634 bp long and included a large 904-bp intron. PSK1 transcription was detected in all tissues, with the highest level observed in sepals, followed by leaves. Under salinity stress, overexpression of PSK1 in Arabidopsis resulted in increased germination percentages, cotyledon greening, and fresh weights relative to wild-type plants. Furthermore, transgenic Arabidopsis lines containing 35S::PSK1 displayed increased expression of genes that would be essential for reproduction and growth under salinity stress: ASK1, LEAFY, FT, and CO involved in flower development and flowering time as well as P5CS, RAB18, DREB, and SOD1-3 contributing to salinity tolerance. Our functional characterization of PSK1 adds to global knowledge of the multiple functions of previously explored SKP1-like genes in plants and sheds light on the molecular mechanism underlying its role in salinity tolerance. Our findings also provide information on the function and molecular mechanism of PSK1 in tree peony flower development, thereby revealing a theoretical basis for regulation of flowering and conferral of salinity tolerance in tree peony.
关键词	SKP1 Paeonia suffruticosa PSK1 Salinity tolerance Flowering
学科领域	Plant Sciences
DOI	10.3389/fpls.2017.01047
收录类别	SCI
语种	英语
WOS关键词	SALT TOLERANCE ; SELF-INCOMPATIBILITY ; STRESS TOLERANCE ; MALE MEIOSIS ; PROTEIN ; EXPRESSION ; SKP1 ; FAMILY ; IDENTIFICATION ; ASK1
WOS记录号	WOS:000403488000001
出版者	SPRINGER
文献子类	Article
出版地	NEW YORK
EISSN	1432-203X
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31272201, 31471909]
作者邮箱	haoqing5608@163.com ; hxren@ibcas.ac.cn ; zhujinb916@163.com ; wanglsh@ibcas.ac.cn ; oppo183@163.com ; liuzhengan@ibcas.ac.cn ; gaozhimin@icbr.ac.cn ; shuqy@ibcas.ac.cn
作品OA属性	gold, Green Published
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22031
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Plant Resources Beijing Bot Garden, Inst Bot, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Agr Univ, Landscape & Forestry Coll, Qingdao 266109, Peoples R China 4.Int Ctr Bamboo & Rattan, Key Lab Sci & Technol Bamboo & Rattan, Beijing 100102, Peoples R China
推荐引用方式 GB/T 7714	Hao, Qing,Ren, Hongxu,Zhu, Jin,et al. Overexpression of PSK1, a SKP1-like gene homologue, from Paeonia suffruticosa, confers salinity tolerance in Arabidopsis[J]. PLANT CELL REPORTS,2017,36(1):151-162.
APA	Hao, Qing.,Ren, Hongxu.,Zhu, Jin.,Wang, Liangsheng.,Huang, Shouchen.,...&Shu, Qingyan.(2017).Overexpression of PSK1, a SKP1-like gene homologue, from Paeonia suffruticosa, confers salinity tolerance in Arabidopsis.PLANT CELL REPORTS,36(1),151-162.
MLA	Hao, Qing,et al."Overexpression of PSK1, a SKP1-like gene homologue, from Paeonia suffruticosa, confers salinity tolerance in Arabidopsis".PLANT CELL REPORTS 36.1(2017):151-162.

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