The SWI2/SNF2 Chromatin-Remodeling ATPase BRAHMA Regulates Chlorophyll Biosynthesis in Arabidopsis

doi:10.1007/s11771-017-3562-1

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	The SWI2/SNF2 Chromatin-Remodeling ATPase BRAHMA Regulates Chlorophyll Biosynthesis in Arabidopsis
	Zhang, Dong 1; Li, Yuhong 1; Zhang, Xinyu 1; Zha, Ping 1; Lin, Rongcheng1
	2017
发表期刊	MOLECULAR PLANT
ISSN	1674-2052
卷号	10 期号:1 页码:155-167
摘要	Chlorophyll biosynthesis is critical for chloroplast development and photosynthesis in plants. Although reactions in the chlorophyll biosynthetic pathway have been largely known, little is known about the regulatory mechanisms of this pathway. In this study, we found that the dark-grown knockout and knockdown mutants as well as RNA-interference transgenic seedlings of BRAHMA (BRM), which encodes an SWI2/SNF2 chromatin-remodeling ATPase, had higher greening rates, accumulated less protochlorophyllide, and produced less reactive oxygen species than Arabidopsis wild-type plants did upon light exposure. The expression of NADPH: protochlorophyllide oxidoreductase A (PORA), PORB, and PORC, which catalyze a key step in chlorophyll biosynthesis, was increased in the brm mutants. We found that BRM physically interacted with the bHLH transcription factor PHYTOCHROME-INTERACTING FACTOR 1 (PIF1) through its N-terminal domains. Furthermore, we demonstrated that BRM was directly recruited to the cis-regulatory regions of PORC, but not of PORA and PORB, at least partially in a PIF1-dependent manner and the level of histone H3 lysine 4 tri-methylation (H3K4me3) at PORC loci was increased in the brm mutant. Taken together, our data indicate that the chromatin-remodeling enzyme BRM modulates PORC expression through interacting with PIF1, providing a novel regulatory mechanism by which plants fine-tune chlorophyll biosynthesis during the transition from heterotrophic to autotrophic growth.
关键词	chromatin remodeling chlorophyll biosynthesis light signaling PIF1 PORC
学科领域	Metallurgy & Metallurgical Engineering
DOI	10.1007/s11771-017-3562-1
收录类别	SCI
语种	英语
WOS关键词	PROTOCHLOROPHYLLIDE-OXIDOREDUCTASE-A ; PHYTOCHROME-INTERACTING FACTOR-1 ; TRANSCRIPTION FACTOR ; HIGHER-PLANTS ; CHLOROPLAST DEVELOPMENT ; PHOTOOXIDATIVE DAMAGE ; SEED-GERMINATION ; GENE-EXPRESSION ; LIGHT ; SEEDLINGS
WOS记录号	WOS:000407259800006
出版者	CELL PRESS
文献子类	Article
出版地	CAMBRIDGE
EISSN	1752-9867
资助机构	National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31325002] ; Ministry of Agriculture of ChinaMinistry of Agriculture, China [2016ZX08009-003]
作者邮箱	rclin@ibcas.ac.cn
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22036
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Dong,Li, Yuhong,Zhang, Xinyu,et al. The SWI2/SNF2 Chromatin-Remodeling ATPase BRAHMA Regulates Chlorophyll Biosynthesis in Arabidopsis[J]. MOLECULAR PLANT,2017,10(1):155-167.
APA	Zhang, Dong,Li, Yuhong,Zhang, Xinyu,Zha, Ping,&Lin, Rongcheng.(2017).The SWI2/SNF2 Chromatin-Remodeling ATPase BRAHMA Regulates Chlorophyll Biosynthesis in Arabidopsis.MOLECULAR PLANT,10(1),155-167.
MLA	Zhang, Dong,et al."The SWI2/SNF2 Chromatin-Remodeling ATPase BRAHMA Regulates Chlorophyll Biosynthesis in Arabidopsis".MOLECULAR PLANT 10.1(2017):155-167.

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