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| Genome-Wide Identification and Functional Characterization of UDP-Glucosyltransferase Genes Involved in Flavonoid Biosynthesis in Glycine max | |
| Yin, Qinggang; Shen, Guoan1; Di, Shaokang; Fan, Cunying; Chang, Zhenzhan2; Pang, Yongzhen | |
| 2017 | |
| 发表期刊 | PLANT AND CELL PHYSIOLOGY
 |
| ISSN | 0032-0781 |
| 卷号 | 58期号:9页码:1558-1572 |
| 摘要 | Flavonoids, natural products abundant in the model legume Glycine max, confer benefits to plants and to animal health. Flavonoids are present in soybean mainly as glycoconjugates. However, the mechanisms of biosynthesis of flavonoid glycosides are largely unknown in G. max. In the present study, 212 putative UDP-glycosyltransferase (UGT) genes were identified in G. max by genome-wide searching. The GmUGT genes were distributed differentially among the 20 chromosomes, and they were expressed in various tissues with distinct expression profiles. We further analyzed the enzymatic activities of 11 GmUGTs that are potentially involved in flavonoid glycosylation, and found that six of them (UGT72X4, UGT72Z3, UGT73C20, UGT88A13, UGT88E19 and UGT92G4) exhibited activity toward flavonol, isoflavone, flavone and flavanol aglycones with different kinetic properties. Among them, UGT72X4, UGT72Z3 and UGT92G4 are flavonol-specific UGTs, and UGT73C20 and UGT88E19 exhibited activity toward both flavonol and isoflavone aglycones. In particular, UGT88A13 exhibited activity toward epicatechin, but not for the flavonol aglycones kaempferol and quercetin. Overexpression of these six GmUGT genes significantly increased the contents of isoflavone and flavonol glucosides in soybean hairy roots. In addition, overexpression of these six GmUGT genes also affected flavonol glycoside contents differently in seedlings and seeds of transgenic Arabidopsis thaliana. We provide valuable information on the identification of all UGT genes in soybean, and candidate GmUGT genes for potential metabolic engineering of flavonoid compounds in both Escherichia coli and plants. |
| 关键词 | Flavonoids Glucosyltransferase Glycine max Hairy roots Soybean |
| 学科领域 | Plant Sciences |
| DOI | 10.3389/fpls.2017.02040 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | LEGUME MEDICAGO-TRUNCATULA ; PLANT GLYCOSYLTRANSFERASES ; ISOFLAVONE 7-O-GLUCOSYLTRANSFERASE ; CRYSTAL-STRUCTURE ; DOWN-REGULATION ; SOYBEAN SEEDS ; L. MERR. ; METABOLISM ; EPICATECHIN ; REVEALS |
| WOS记录号 | WOS:000416311500001 |
| 出版者 | OXFORD UNIV PRESS |
| 文献子类 | Article |
| 出版地 | OXFORD |
| EISSN | 1471-9053 |
| 资助机构 | Major State Basic Research and Development Program of ChinaNational Basic Research Program of China [2013CB127002] ; National Key Research and Development Program of China [2016YFD0101005] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31670305] |
| 作者邮箱 | yzhpang@ibcas.ac.cn |
| 作品OA属性 | gold, Green Published |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/22037 |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol & Physiol, Beijing 100093, Peoples R China 3.Peking Univ, Sch Basic Med Sci, Dept Biophys, Beijing 100191, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Qinggang,Shen, Guoan,Di, Shaokang,et al. Genome-Wide Identification and Functional Characterization of UDP-Glucosyltransferase Genes Involved in Flavonoid Biosynthesis in Glycine max[J]. PLANT AND CELL PHYSIOLOGY,2017,58(9):1558-1572. |
| APA | Yin, Qinggang,Shen, Guoan,Di, Shaokang,Fan, Cunying,Chang, Zhenzhan,&Pang, Yongzhen.(2017).Genome-Wide Identification and Functional Characterization of UDP-Glucosyltransferase Genes Involved in Flavonoid Biosynthesis in Glycine max.PLANT AND CELL PHYSIOLOGY,58(9),1558-1572. |
| MLA | Yin, Qinggang,et al."Genome-Wide Identification and Functional Characterization of UDP-Glucosyltransferase Genes Involved in Flavonoid Biosynthesis in Glycine max".PLANT AND CELL PHYSIOLOGY 58.9(2017):1558-1572. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| 29234343.pdf（2106KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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