Isolation of three cyanins from Lonicera caerulea L. fruits and its anticancer activity

doi:10.1007/s10533-017-0396-8

	Isolation of three cyanins from Lonicera caerulea L. fruits and its anticancer activity
	Luo Zhou-fei ; Yang Yuan ; Wang Liang-sheng; Li Hai-pu
	2017
发表期刊	JOURNAL OF CENTRAL SOUTH UNIVERSITY
ISSN	2095-2899
卷号	24 期号:7 页码:1573-1581
摘要	Lonicera caerulea L. fruit is an excellent source of bioactive compounds. An efficient separation method of cyanins is important for the development of many value-added products and functional food ingredients. High-speed counter-current chromatography (HSCCC) was applied to isolate cyanins from Lonicera caerulea fruits with a biphasic solvent system composed of methyl tert-butyl ether/n-butanol/acetonitrile/water/trifluoroacetic acid (2:2:1:5:0.01, volume ratio). 1.41 mg of cyanidin 3, 5-O-diglucoside, 1.08 mg of cyanidin 3-O-rutinoside and 6.38 mg of cyanidin 3-O-glucoside were obtained from 40 mg of crude extract. The purities of these compounds were 95.8%, 92.4% and 97.6%, respectively, as identified by high-performance liquid chromatography-diode array detection (HPLC-DAD) and high-performance liquid chromatography-electrospray ionization mass spectrometry(n) (HPLC-ESI/MSn). In addition, the dominant anthocyanin, cyanidin 3-O-glucoside was demonstrated cytotoxic response of human hepatocarcinoma SMMC-7721 cells, inducing live cancer cell apoptotic by flow cytometric analysis.
关键词	cyanins cyanidin 3-O-glucoside isolation anticancer HSCCC HPLC-ESI-MSn
学科领域	Environmental Sciences & Ecology ; Geology
DOI	10.1007/s10533-017-0396-8
收录类别	SCI
语种	英语
WOS关键词	COUNTER-CURRENT CHROMATOGRAPHY ; LIQUID-CHROMATOGRAPHY ; BERRY FRUITS ; ANTHOCYANINS ; ANTIOXIDANT ; PURIFICATION ; 3-GLUCOSIDE ; EXTRACTION ; SEPARATION ; PHENOLICS
WOS记录号	WOS:000416325100006
出版者	JOURNAL OF CENTRAL SOUTH UNIV TECHNOLOGY
文献子类	Article
出版地	HUNAN
EISSN	1993-0666
资助机构	Knowledge Innovation Program of the Chinese Academy of Sciences, China [KSCX2-YW-N-043]
作者邮箱	wanglsh@ibcas.ac.cn ; lihaipu@csu.edu.cn
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22038
专题	中科院北方资源植物重点实验室
作者单位	1.[Luo Zhou-fei 2.Yang Yuan 3.Cent S Univ, Coll Chem & Chem Engn, Ctr Environm & Water Resources, Changsha 410083, Hunan, Peoples R China 4.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Key Lab Plant Resources, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Luo Zhou-fei,Yang Yuan,Wang Liang-sheng,et al. Isolation of three cyanins from Lonicera caerulea L. fruits and its anticancer activity[J]. JOURNAL OF CENTRAL SOUTH UNIVERSITY,2017,24(7):1573-1581.
APA	Luo Zhou-fei,Yang Yuan,Wang Liang-sheng,&Li Hai-pu.(2017).Isolation of three cyanins from Lonicera caerulea L. fruits and its anticancer activity.JOURNAL OF CENTRAL SOUTH UNIVERSITY,24(7),1573-1581.
MLA	Luo Zhou-fei,et al."Isolation of three cyanins from Lonicera caerulea L. fruits and its anticancer activity".JOURNAL OF CENTRAL SOUTH UNIVERSITY 24.7(2017):1573-1581.

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