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| Two SUMO Proteases SUMO PROTEASE RELATED TO FERTILITY1 and 2 Are Required for Fertility in Arabidopsis | |
| Liu, Linpo1; Jiang, Ying1,2; Zhang, Xiaomei; Wang, Xu; Wang, Yanbing3; Han, Yuzhen1; Coupland, George4; Jin, Jing Bo5; Searle, Iain6; Fu, Yong-Fu; Chen, Fulu | |
| 2017 | |
| 发表期刊 | PLANT PHYSIOLOGY
 |
| ISSN | 0032-0889 |
| 卷号 | 175期号:4页码:1703-1719 |
| 摘要 | In plants, the posttranslational modification small ubiquitin-like modifier (SUMO) is involved in regulating several important developmental and cellular processes, including flowering time control and responses to biotic and abiotic stresses. Here, we report two proteases, SUMO PROTEASE RELATED TO FERTILITY1 (SPF1) and SPF2, that regulate male and female gamete and embryo development and remove SUMO from proteins in vitro and in vivo. spf1 mutants exhibit abnormal floral structures and embryo development, while spf2 mutants exhibit largely a wild-type phenotype. However, spf1 spf2 double mutants exhibit severe abnormalities in microgametogenesis, megagametogenesis, and embryo development, suggesting that the two genes are functionally redundant. Mutation of SPF1 and SPF2 genes also results in misexpression of generative-and embryo-specific genes. In vitro, SPF1 and SPF2 process SUMO1 precursors into a mature form, and as expected in vivo, spf1 and spf2 mutants accumulate SUMO conjugates. Using a yeast two-hybrid screen, we identified EMBRYO SAC DEVELOPMENT ARREST9 (EDA9) as an SPF1-interacting protein. In vivo, we demonstrate that EDA9 is sumolyated and that, in spf1 mutants, EDA9-SUMO conjugates increase in abundance, demonstrating that EDA9 is a substrate of SPF1. Together, our results demonstrate that SPF1 and SPF2 are two SUMO proteases important for plant development in Arabidopsis (Arabidopsis thaliana). |
| 学科领域 | Biodiversity & Conservation ; Environmental Sciences & Ecology |
| DOI | 10.1111/gcb.13789 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | E3 LIGASE SIZ1 ; CELL-CYCLE PROGRESSION ; UBIQUITIN-CONJUGATING ENZYME ; ABSCISIC-ACID ; FEMALE GAMETOPHYTE ; SALICYLIC ACID ; CAENORHABDITIS-ELEGANS ; SPIKELET FERTILITY ; FLOWERING PLANTS ; REFERENCE GENES |
| WOS记录号 | WOS:000414969000025 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| 文献子类 | Article |
| 出版地 | ROCKVILLE |
| EISSN | 1532-2548 |
| 资助机构 | Chinese National Science FoundationNational Natural Science Foundation of China (NSFC) [30670189, 31370324] ; Beijing National Science Foundation [5132031] ; CAAS ; CAAS Special Funding of National Non-Profit Institutes ; Agricultural Science and Technology Innovation Program ; Australian Research CouncilAustralian Research Council [FT130100525] |
| 作者邮箱 | fuyongfu@caas.cn ; chenfulu@caas.cn |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/22052 |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Agr Sci, MOA Key Lab Soybean Biol Beijing, Natl Key Facil Crop Gene Resource & Genet Improve, Inst Crop Sci, Beijing 100081, Peoples R China 2.China Agr Univ, State Key Lab Plant Physiol & Biochem, Coll Biol Sci, Beijing 100094, Peoples R China 3.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing 100097, Peoples R China 4.Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China 5.Max Planck Inst Plant Breeding, D-50829 Cologne, Germany 6.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 7.Univ Adelaide, Univ Adelaide Shanghai Jiao Tong Univ Joint Ctr A, Sch Biol Sci, Adelaide, SA 5005, Australia |
| 推荐引用方式 GB/T 7714 | Liu, Linpo,Jiang, Ying,Zhang, Xiaomei,et al. Two SUMO Proteases SUMO PROTEASE RELATED TO FERTILITY1 and 2 Are Required for Fertility in Arabidopsis[J]. PLANT PHYSIOLOGY,2017,175(4):1703-1719. |
| APA | Liu, Linpo.,Jiang, Ying.,Zhang, Xiaomei.,Wang, Xu.,Wang, Yanbing.,...&Chen, Fulu.(2017).Two SUMO Proteases SUMO PROTEASE RELATED TO FERTILITY1 and 2 Are Required for Fertility in Arabidopsis.PLANT PHYSIOLOGY,175(4),1703-1719. |
| MLA | Liu, Linpo,et al."Two SUMO Proteases SUMO PROTEASE RELATED TO FERTILITY1 and 2 Are Required for Fertility in Arabidopsis".PLANT PHYSIOLOGY 175.4(2017):1703-1719. |
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