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The Phytol Phosphorylation Pathway Is Essential for the Biosynthesis of Phylloquinone, which Is Required for Photosystem I Stability in Arabidopsis
Wang, Lei1; Li, Qingwei1; Zhang, Aihong; Zhou, Wen1; Jiang, Rui1; Yang, Zhipan; Yang, Huixia; Qin, Xiaochun; Ding, Shunhua; Lu, Qingtao; Wen, Xiaogang; Lu, Congming2
2017
发表期刊MOLECULAR PLANT
ISSN1674-2052
卷号10期号:1页码:183-196
摘要Phytyl-diphosphate, which provides phytyl moieties as a common substrate in both tocopherol and phylloquinone biosynthesis, derives from de novo isoprenoid biosynthesis or a salvage pathway via phytol phosphorylation. However, very little is known about the role and origin of the phytyl moiety for phylloquinone biosynthesis. Since VTE6, a phytyl-phosphate kinase, is a key enzyme for phytol phosphorylation, we characterized Arabidopsis vte6 mutants to gain insight into the roles of phytyl moieties in phylloquinone biosynthesis and of phylloquinone in photosystem I (PSI) biogenesis. The VTE6 knockout mutants vte6-1 and vte6-2 lacked detectable phylloquinone, whereas the phylloquinone content in the VTE6 knockdown mutant vte6-3 was 90% lower than that in wild-type. In vte6 mutants, PSI function was impaired and accumulation of the PSI complex was defective. The PSI core subunits PsaA/B were efficiently synthesized and assembled into the PSI complex in vte6-3. However, the degradation rate of PSI subunits in the assembled PSI complex was more rapid in vte6-3 than in wild-type. In vte6-3, PSI was more susceptible to high-light damage than in wild-type. Our results provide the first genetic evidence that the phytol phosphorylation pathway is essential for phylloquinone biosynthesis, and that phylloquinone is required for PSI complex stability.
关键词phytol phosphorylation VTE6 phylloquinone PSI stability
学科领域Chemistry ; Food Science & Technology ; Nutrition & Dietetics
DOI10.1016/j.foodchem.2017.05.127
收录类别SCI
语种英语
WOS关键词ELECTRON-TRANSFER ; FOREIGN QUINONE ; TOCOPHEROL BIOSYNTHESIS ; CHLOROPLAST DEVELOPMENT ; ANGSTROM RESOLUTION ; PLASTOQUINONE-POOL ; THYLAKOID PROTEIN ; MUTANT REVEALS ; A(1) SITE ; VITAMIN-E
WOS记录号WOS:000406199900047
出版者CELL PRESS
文献子类Article
出版地CAMBRIDGE
EISSN1752-9867
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31370274] ; Key Research Plan of Frontier Sciences of the Chinese Academy of Sciences [QYZDJ-SSW-SMC003] ; State Key Basic Research and Development Plan of ChinaNational Basic Research Program of China [2015CB150105] ; Strategic Priority Research Program of the Chinese Academy Sciences [XDB17030100]
作者邮箱lucm@ibcas.ac.cn
引用统计
被引频次:48[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/22061
专题中科院光生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynth Res Ctr, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Natl Ctr Plant Gene Res, Beijing 100093, Peoples R China
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GB/T 7714
Wang, Lei,Li, Qingwei,Zhang, Aihong,et al. The Phytol Phosphorylation Pathway Is Essential for the Biosynthesis of Phylloquinone, which Is Required for Photosystem I Stability in Arabidopsis[J]. MOLECULAR PLANT,2017,10(1):183-196.
APA Wang, Lei.,Li, Qingwei.,Zhang, Aihong.,Zhou, Wen.,Jiang, Rui.,...&Lu, Congming.(2017).The Phytol Phosphorylation Pathway Is Essential for the Biosynthesis of Phylloquinone, which Is Required for Photosystem I Stability in Arabidopsis.MOLECULAR PLANT,10(1),183-196.
MLA Wang, Lei,et al."The Phytol Phosphorylation Pathway Is Essential for the Biosynthesis of Phylloquinone, which Is Required for Photosystem I Stability in Arabidopsis".MOLECULAR PLANT 10.1(2017):183-196.
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