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The Phytol Phosphorylation Pathway Is Essential for the Biosynthesis of Phylloquinone, which Is Required for Photosystem I Stability in Arabidopsis | |
Wang, Lei1; Li, Qingwei1; Zhang, Aihong; Zhou, Wen1; Jiang, Rui1; Yang, Zhipan; Yang, Huixia; Qin, Xiaochun; Ding, Shunhua; Lu, Qingtao; Wen, Xiaogang; Lu, Congming2 | |
2017 | |
发表期刊 | MOLECULAR PLANT |
ISSN | 1674-2052 |
卷号 | 10期号:1页码:183-196 |
摘要 | Phytyl-diphosphate, which provides phytyl moieties as a common substrate in both tocopherol and phylloquinone biosynthesis, derives from de novo isoprenoid biosynthesis or a salvage pathway via phytol phosphorylation. However, very little is known about the role and origin of the phytyl moiety for phylloquinone biosynthesis. Since VTE6, a phytyl-phosphate kinase, is a key enzyme for phytol phosphorylation, we characterized Arabidopsis vte6 mutants to gain insight into the roles of phytyl moieties in phylloquinone biosynthesis and of phylloquinone in photosystem I (PSI) biogenesis. The VTE6 knockout mutants vte6-1 and vte6-2 lacked detectable phylloquinone, whereas the phylloquinone content in the VTE6 knockdown mutant vte6-3 was 90% lower than that in wild-type. In vte6 mutants, PSI function was impaired and accumulation of the PSI complex was defective. The PSI core subunits PsaA/B were efficiently synthesized and assembled into the PSI complex in vte6-3. However, the degradation rate of PSI subunits in the assembled PSI complex was more rapid in vte6-3 than in wild-type. In vte6-3, PSI was more susceptible to high-light damage than in wild-type. Our results provide the first genetic evidence that the phytol phosphorylation pathway is essential for phylloquinone biosynthesis, and that phylloquinone is required for PSI complex stability. |
关键词 | phytol phosphorylation VTE6 phylloquinone PSI stability |
学科领域 | Chemistry ; Food Science & Technology ; Nutrition & Dietetics |
DOI | 10.1016/j.foodchem.2017.05.127 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | ELECTRON-TRANSFER ; FOREIGN QUINONE ; TOCOPHEROL BIOSYNTHESIS ; CHLOROPLAST DEVELOPMENT ; ANGSTROM RESOLUTION ; PLASTOQUINONE-POOL ; THYLAKOID PROTEIN ; MUTANT REVEALS ; A(1) SITE ; VITAMIN-E |
WOS记录号 | WOS:000406199900047 |
出版者 | CELL PRESS |
文献子类 | Article |
出版地 | CAMBRIDGE |
EISSN | 1752-9867 |
资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31370274] ; Key Research Plan of Frontier Sciences of the Chinese Academy of Sciences [QYZDJ-SSW-SMC003] ; State Key Basic Research and Development Plan of ChinaNational Basic Research Program of China [2015CB150105] ; Strategic Priority Research Program of the Chinese Academy Sciences [XDB17030100] |
作者邮箱 | lucm@ibcas.ac.cn |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/22061 |
专题 | 中科院光生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynth Res Ctr, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Natl Ctr Plant Gene Res, Beijing 100093, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Lei,Li, Qingwei,Zhang, Aihong,et al. The Phytol Phosphorylation Pathway Is Essential for the Biosynthesis of Phylloquinone, which Is Required for Photosystem I Stability in Arabidopsis[J]. MOLECULAR PLANT,2017,10(1):183-196. |
APA | Wang, Lei.,Li, Qingwei.,Zhang, Aihong.,Zhou, Wen.,Jiang, Rui.,...&Lu, Congming.(2017).The Phytol Phosphorylation Pathway Is Essential for the Biosynthesis of Phylloquinone, which Is Required for Photosystem I Stability in Arabidopsis.MOLECULAR PLANT,10(1),183-196. |
MLA | Wang, Lei,et al."The Phytol Phosphorylation Pathway Is Essential for the Biosynthesis of Phylloquinone, which Is Required for Photosystem I Stability in Arabidopsis".MOLECULAR PLANT 10.1(2017):183-196. |
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