PSA3, a Protein on the Stromal Face of the Thylakoid Membrane, Promotes Photosystem I Accumulation in Cooperation with the Assembly Factor PYG7(1[OPEN])

doi:10.1111/mec.14396

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	PSA3, a Protein on the Stromal Face of the Thylakoid Membrane, Promotes Photosystem I Accumulation in Cooperation with the Assembly Factor PYG7(1[OPEN])
	Shen, Jie ; Williams-Carrier, Rosalind ; Barkan, Alice 1
	2017
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
卷号	174 期号:3 页码:1850-1862
摘要	PSI is a large protein-pigment complex located in the thylakoid membrane in cyanobacteria, plants, and algae. Although the structure and components of PSI are well characterized, mechanisms that orchestrate its assembly are poorly understood. In this study, we discovered a novel nucleus-encoded protein, Photosystem I Assembly3 (PSA3), that is required for PSI accumulation. PSA3 is conserved among green photosynthetic eukaryotes but is lacking in cyanobacteria. Mutations in the psa3 gene cause the specific loss of PSI in Arabidopsis (Arabidopsis thaliana) and maize (Zea mays). Ribosome profiling and pulse-labeling analyses showed that chloroplast-encoded PSI subunits are synthesized at normal rates in psa3 mutants, indicating that PSA3 is involved in the biogenesis of PSI at a posttranslational step. PSA3 resides on the stromal face of the thylakoid membrane, where it is found in a complex that is slightly smaller than PSI. Structural predictions suggest that PSA3 binds a basic peptide in a manner that is sensitive to the oxidation state of Cys pairs flanking the predicted peptide binding groove. PSA3 and the previously described PSI biogenesis factor PYG7 interact in yeast two-hybrid and bimolecular fluorescence complementation assays, and they are found in thylakoid membrane complexes of similar size. These and other results indicate that PSA3 cooperates with PYG7 to promote the stable assembly of PSI, and that the PsaC subunit is likely to be the primary target of their action.
学科领域	Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
DOI	10.1111/mec.14396
收录类别	SCI
语种	英语
WOS关键词	CHLOROPLAST BIOGENESIS ; SPLIT-UBIQUITIN ; ARABIDOPSIS ; DOMAIN ; YCF3 ; IDENTIFICATION ; INTERMEDIATE ; PHENOTYPE ; DATABASE ; TOBACCO
WOS记录号	WOS:000418252300009
出版者	AMER SOC PLANT BIOLOGISTS
文献子类	Article
出版地	ROCKVILLE
EISSN	1532-2548
资助机构	Chinese Academy of SciencesChinese Academy of Sciences ; Division Of Integrative Organismal SystemsNational Science Foundation (NSF)NSF - Directorate for Biological Sciences (BIO) [1339130] Funding Source: National Science Foundation
作者邮箱	abarkan@uoregon.edu
作品OA属性	Green Published
引用统计	被引频次：70[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22062
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Oregon, Inst Mol Biol, Eugene, OR 97403 USA
推荐引用方式 GB/T 7714	Shen, Jie,Williams-Carrier, Rosalind,Barkan, Alice. PSA3, a Protein on the Stromal Face of the Thylakoid Membrane, Promotes Photosystem I Accumulation in Cooperation with the Assembly Factor PYG7(1[OPEN])[J]. PLANT PHYSIOLOGY,2017,174(3):1850-1862.
APA	Shen, Jie,Williams-Carrier, Rosalind,&Barkan, Alice.(2017).PSA3, a Protein on the Stromal Face of the Thylakoid Membrane, Promotes Photosystem I Accumulation in Cooperation with the Assembly Factor PYG7(1[OPEN]).PLANT PHYSIOLOGY,174(3),1850-1862.
MLA	Shen, Jie,et al."PSA3, a Protein on the Stromal Face of the Thylakoid Membrane, Promotes Photosystem I Accumulation in Cooperation with the Assembly Factor PYG7(1[OPEN])".PLANT PHYSIOLOGY 174.3(2017):1850-1862.

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