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| GmMYB58 and GmMYB205 are seed-specific activators for isoflavonoid biosynthesis in Glycine max | |
| Han, Xiaoyan1; Yin, Qinggang1; Liu, Jinyue1; Jiang, Wenbo; Di, Shaokang; Pang, Yongzhen | |
| 2017 | |
| 发表期刊 | PLANT CELL REPORTS
 |
| ISSN | 0721-7714 |
| 卷号 | 36期号:12页码:1889-1902 |
| 摘要 | GmMYB58 and GmMYB205 are key positive regulators that are involved in isoflavonoid biosynthesis in seeds of Glycine max, and they activate the expression of several structural genes in the isoflavonoid pathway. MYB transcription factors (TFs) are major regulators involved in flavonoid/isoflavonoid biosynthesis in many plant species. However, functions of most MYB TFs remain unknown in flavonoid/isoflavonoid pathway in Glycine max. In this study, we identified 321 MYB TFs by genome-wide searching, and further isolated and functionally characterized two MYB TFs, GmMYB58 and GmMYB205. The deduced GmMYB58 and GmMYB205 proteins contain highly conserved R2R3 repeat domain at the N-terminal region that is the signature motif of R2R3-type MYB TFs. GmMYB58 and GmMYB205 were highly expressed in early seed development stages than in the other tested organs. GmMYB58 and GmMYB205 GFP fusion proteins were found to be localized in the nucleus when they were transiently expressed in Arabidopsis thaliana mesophyll protoplast. Both GmMYB58 and GmMYB205 can activate the promoter activities of GmCHS, GmIFS2, and GmHID in the transient trans-activation assays, and the activation of GmHID by both GmMYB58 and GmMYB205 was further confirmed by yeast one-hybrid assay. In addition, over-expression of GmMYB58 and GmMYB205 resulted in significant increases in expression levels of several pathway genes in soybean hairy roots, in particular, IFS2 by more than fivefolds in GmMYB205-over-expressing lines. Moreover, isoflavonoid contents were remarkably enhanced in the GmMYB58 and GmMYB205 over-expressing hairy roots than in the control. Our results suggest that GmMYB58 and GmMYB205 are seed-specific TFs, and they can enhance isoflavonoid biosynthesis mainly through the regulation of GmIFS2 and GmHID in G. max. |
| 关键词 | Glycine max Hairy roots Isoflavonoid biosynthesis MYB transcription factors |
| 学科领域 | Forestry |
| DOI | 10.1016/j.foreco.2017.10.008 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | R2R3-MYB TRANSCRIPTION FACTOR ; DEVELOPING SOYBEAN SEEDS ; MYB DOMAIN ; PROANTHOCYANIDIN BIOSYNTHESIS ; FLAVONOID BIOSYNTHESIS ; ARABIDOPSIS-THALIANA ; GENE-EXPRESSION ; DNA-BINDING ; MAIZE C1 ; PROTEINS |
| WOS记录号 | WOS:000416395800007 |
| 出版者 | SPRINGER |
| 文献子类 | Article |
| 出版地 | NEW YORK |
| EISSN | 1432-203X |
| 资助机构 | National Key Research and Development Program of China [2016YFD0101005] ; Major State Basic Research and Development Program of ChinaNational Basic Research Program of China [2013CB127002] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31670305] |
| 作者邮箱 | yzhpang@ibcas.ac.cn |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/22066 |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Xiaoyan,Yin, Qinggang,Liu, Jinyue,et al. GmMYB58 and GmMYB205 are seed-specific activators for isoflavonoid biosynthesis in Glycine max[J]. PLANT CELL REPORTS,2017,36(12):1889-1902. |
| APA | Han, Xiaoyan,Yin, Qinggang,Liu, Jinyue,Jiang, Wenbo,Di, Shaokang,&Pang, Yongzhen.(2017).GmMYB58 and GmMYB205 are seed-specific activators for isoflavonoid biosynthesis in Glycine max.PLANT CELL REPORTS,36(12),1889-1902. |
| MLA | Han, Xiaoyan,et al."GmMYB58 and GmMYB205 are seed-specific activators for isoflavonoid biosynthesis in Glycine max".PLANT CELL REPORTS 36.12(2017):1889-1902. |
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| j.foreco.2017.10.008（723KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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