Phosphoproteomic Analysis of Paper Mulberry Reveals Phosphorylation Functions in Chilling Tolerance

doi:10.1016/j.scitotenv.2017.07.082

	Phosphoproteomic Analysis of Paper Mulberry Reveals Phosphorylation Functions in Chilling Tolerance
	Pi, Zhi ; Zhao, Mei-Ling ; Peng, Xian-Jun ; Shen, Shi-Hua
	2017
发表期刊	JOURNAL OF PROTEOME RESEARCH
ISSN	1535-3893
卷号	16 期号:5 页码:1944-1961
摘要	Paper mulberry is a valuable woody species with a good chilling tolerance. In this study, phosphoproteomic analysis, physiological measurement, and mRNA quantification were employed to explore the molecular mechanism of chilling (4 degrees C) tolerance in paper mulberry. After chilling for 6 h, 427 significantly changed phosphoproteins were detected in paper mulberry seedlings without obvious physiological injury. When obvious physiological injury occurred after chilling for 48 h, a total of 611 phosphoproteins were found to be significantly changed at the phosphorylation level. Several protein kinases, especially CKII, were possibly responsible for these changes according to conserved sequence analysis. The results of Gene Ontology analysis showed that phosphoproteins were mainly responsible for signal transduction, protein modification, and translation during chilling. Additionally, transport and cellular component organization were enriched after chilling for 6 and 48 h, respectively. On the basis of the protein protein interaction network analysis, a protein kinase and phosphatases hub protein (P1959) were found to be involved in cross-talk between Ca2+, BR, ABA, and ethylene-mediated signaling pathways. We also highlighted the phosphorylation of BpSIZ1 and BpICE1 possibly impacted on the CBF/DREB-responsive pathway. From these results, we developed a schematic for the chilling tolerance mechanism at phosphorylation level.
关键词	chilling tolerance phosphorylation paper mulberry hub protein CBF/DREB-responsive pathway
学科领域	Environmental Sciences & Ecology
DOI	10.1016/j.scitotenv.2017.07.082
收录类别	SCI
语种	英语
WOS关键词	BLUE-LIGHT RECEPTOR ; ABSCISIC-ACID ABA ; LOW-TEMPERATURE ; CHLOROPLAST MOVEMENT ; COLD-ACCLIMATION ; QUANTITATIVE PHOSPHOPROTEOMICS ; PROTEIN-KINASE ; COMPREHENSIVE ANALYSIS ; PROTEOMIC ANALYSIS ; PROFILING REVEALS
WOS记录号	WOS:000408755300117
出版者	AMER CHEMICAL SOC
文献子类	Article
出版地	WASHINGTON
EISSN	1535-3907
资助机构	Poverty Relief Project of the Chinese Academy of Sciences [KFJ-FP-24] ; Huimin Technology Demonstration Project of the National Modern Agricultural Science and Technology Achievements City [Z151100001015008] ; Knowledge Innovation Program through the Chinese Academy of SciencesKnowledge Innovation Program of the Chinese Academy of Sciences [KZCX2-YW-359-2] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270653]
作者邮箱	shshen@ibcas.ac.cn
引用统计	被引频次：62[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22072
专题	中科院北方资源植物重点实验室
作者单位	1.[Pi, Zhi 2.Zhao, Mei-Ling 3.Peng, Xian-Jun 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Pi, Zhi,Zhao, Mei-Ling,Peng, Xian-Jun,et al. Phosphoproteomic Analysis of Paper Mulberry Reveals Phosphorylation Functions in Chilling Tolerance[J]. JOURNAL OF PROTEOME RESEARCH,2017,16(5):1944-1961.
APA	Pi, Zhi,Zhao, Mei-Ling,Peng, Xian-Jun,&Shen, Shi-Hua.(2017).Phosphoproteomic Analysis of Paper Mulberry Reveals Phosphorylation Functions in Chilling Tolerance.JOURNAL OF PROTEOME RESEARCH,16(5),1944-1961.
MLA	Pi, Zhi,et al."Phosphoproteomic Analysis of Paper Mulberry Reveals Phosphorylation Functions in Chilling Tolerance".JOURNAL OF PROTEOME RESEARCH 16.5(2017):1944-1961.

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