Knowledge Management System Of Institute Of Botany,CAS
| Identification and characterization of Rubisco activase genes in Oryza punctata | |
| Xu, Si; Qin, Zong-Yan; Gong, Pi-Chang; Dong, Qian-Li; Bao, Ying2 | |
| 2017 | |
| 发表期刊 | JOURNAL OF SYSTEMATICS AND EVOLUTION
 |
| ISSN | 1674-4918 |
| 卷号 | 55期号:3页码:200-207 |
| 摘要 | Rubisco activase (Rca), a specific chaperone, catalyzes the in vivo activation of Rubisco, and thus plays a major role in plant photosynthesis. Although the genes encoding Rubisco activase have been studied in many model or economic plants, few studies have analyzed their homologs in plants of closely related crop species. In this study, an Rca gene was identified and characterized in a wild relative of rice, Oryza punctata Kotschy ex Steud. The gene was 2747 bp long and possessed six exons and five introns with 47% GC content. Furthermore, cDNA sequencing produced two transcripts, RcaL and RcaS, that differed in the sequence by an inclusion of 99 bp at the carboxy terminus of RcaL. Sequence comparison between the two transcripts and the genomic DNA showed that there was a 20-bp alternative splicing event that occurred at the fifth intron of the gene leading to the synthesis of a short polypeptide. The leaf transcriptome analysis showed that RcaS had a higher expression level than that of RcaL in a normal growth environment. In addition, the yeast two-hybrid assays showed the small isoform of Rubisco activase in close contact with the large subunit of the Rubisco in this species, supporting the side-on binding model of interaction between two proteins. This study broadens our understanding of the molecular characteristics of some essential genes in photosynthesis. |
| 关键词 | Rubisco activase alternative splicing wild rice expression divergence protein interaction |
| 学科领域 | Computer Science ; Geography ; Physical Geography ; Information Science & Library Science |
| DOI | 10.1080/13658816.2016.1195502 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | RIBULOSE 1,5-BISPHOSPHATE ; CARBOXYLASE/OXYGENASE ACTIVASE ; EXPRESSION ; PLANTS ; PHOTOSYNTHESIS ; SUBSTITUTION ; ALIGNMENT ; ISOFORMS ; ENZYMES ; MAIZE |
| WOS记录号 | WOS:000387268600010 |
| 出版者 | WILEY |
| 文献子类 | Article |
| 出版地 | HOBOKEN |
| EISSN | 1759-6831 |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31570218] |
| 作者邮箱 | baoyingus@126.com |
| 作品OA属性 | Green Published, hybrid |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/22074 |
| 专题 | 系统与进化植物学国家重点实验室 |
| 作者单位 | 1.[Xu, Si 2.Qin, Zong-Yan 3.Qufu Normal Univ, Sch Life Sci, Qufu 273165, Shandong, Peoples R China 4.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 5.Northeast Normal Univ, Key Lab Mol Epigenet, Minist Educ, Changchun 130024, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Si,Qin, Zong-Yan,Gong, Pi-Chang,et al. Identification and characterization of Rubisco activase genes in Oryza punctata[J]. JOURNAL OF SYSTEMATICS AND EVOLUTION,2017,55(3):200-207. |
| APA | Xu, Si,Qin, Zong-Yan,Gong, Pi-Chang,Dong, Qian-Li,&Bao, Ying.(2017).Identification and characterization of Rubisco activase genes in Oryza punctata.JOURNAL OF SYSTEMATICS AND EVOLUTION,55(3),200-207. |
| MLA | Xu, Si,et al."Identification and characterization of Rubisco activase genes in Oryza punctata".JOURNAL OF SYSTEMATICS AND EVOLUTION 55.3(2017):200-207. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| 13658816.2016.119550（3507KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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