Isolation and characterization of PSI-LHCI super-complex and their sub-complexes from a red alga Cyanidioschyzon merolae

doi:10.1111/jvs.12464

IB-CAS > 中科院光生物学重点实验室

	Isolation and characterization of PSI-LHCI super-complex and their sub-complexes from a red alga Cyanidioschyzon merolae
	Tian, Lirong 1; Liu, Zheyi 2; Wang, Fangjun 2; Shen, Liangliang 1; Chen, Jinghua 1; Chang, Lijing 1; Zhao, Songhao 1; Han, Guangye ; Wang, Wenda ; Kuang, Tingyun ; Qin, Xiaochun 3; Shen, Jian-Ren
	2017
发表期刊	PHOTOSYNTHESIS RESEARCH
ISSN	0166-8595
卷号	133 期号:1-3 页码:201-214
摘要	Photosystem I (PSI)-light-harvesting complex I (LHCI) super-complex and its sub-complexes PSI core and LHCI, were purified from a unicellular red alga Cyanidioschyzon merolae and characterized. PSI-LHCI of C. merolae existed as a monomer with a molecular mass of 580 kDa. Mass spectrometry analysis identified 11 subunits (PsaA, B, C, D, E, F, I, J, K, L, O) in the core complex and three LHCI subunits, CMQ142C, CMN234C, and CMN235C in LHCI, indicating that at least three Lhcr subunits associate with the red algal PSI core. PsaG was not found in the red algae PSI-LHCI, and we suggest that the position corresponding to Lhca1 in higher plant PSI-LHCI is empty in the red algal PSI-LHCI. The PSI-LHCI complex was separated into two bands on native PAGE, suggesting that two different complexes may be present with slightly different protein compositions probably with respective to the numbers of Lhcr subunits. Based on the results obtained, a structural model was proposed for the red algal PSI-LHCI. Furthermore, pigment analysis revealed that the C. merolae PSI-LHCI contained a large amount of zeaxanthin, which is mainly associated with the LHCI complex whereas little zeaxanthin was found in the PSI core. This indicates a unique feature of the carotenoid composition of the Lhcr proteins and may suggest an important role of Zea in the light-harvesting and photoprotection of the red algal PSI-LHCI complex.
关键词	Cyanidioschyzon merolae PSI-LHCI Light-harvesting Zeaxanthin Red algae
学科领域	Plant Sciences ; Environmental Sciences & Ecology ; Forestry
DOI	10.1111/jvs.12464
收录类别	SCI
语种	英语
WOS关键词	PLANT PHOTOSYSTEM-I ; LIGHT-HARVESTING COMPLEXES ; ENERGY-TRANSFER PATHWAYS ; CHLAMYDOMONAS-REINHARDTII ; GALDIERIA-SULPHURARIA ; PORPHYRIDIUM-CRUENTUM ; CYANIDIUM-CALDARIUM ; GREEN-ALGA ; SUBUNIT ; SUPERCOMPLEX
WOS记录号	WOS:000395422900014
出版者	SPRINGER
Special Issue	SI
文献子类	Article; Proceedings Paper
出版地	DORDRECHT
EISSN	1573-5079
资助机构	Strategic Priority Research Program of CAS [XDB17030100] ; China State Key Basic Research Program Grants [2015CB150100, 2013CB911203] ; CAS Key Research Project for Frontier Science [QYZDY-SSW-SMC003] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31622007, 31670237]
作者邮箱	bio_qinxc@ujn.edu.cn ; shen@cc.okayama-u.ac.jp
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22075
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Photosynthesis Res Ctr, Key Lab Photobiol, 20 Nanxincun, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Yuquan Rd, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Zhongshan Rd 457, Dalian 116023, Peoples R China 4.Univ Jinan, Sch Biol Sci & Technol, 336 Nanxinzhuang West Rd, Jinan 250022, Peoples R China 5.Okayama Univ, Res Inst Interdisciplinary Sci, Grad Sch Nat Sci & Technol, Tsushima Naka 3-1-1, Okayama 7008530, Japan
推荐引用方式 GB/T 7714	Tian, Lirong,Liu, Zheyi,Wang, Fangjun,et al. Isolation and characterization of PSI-LHCI super-complex and their sub-complexes from a red alga Cyanidioschyzon merolae[J]. PHOTOSYNTHESIS RESEARCH,2017,133(1-3):201-214.
APA	Tian, Lirong.,Liu, Zheyi.,Wang, Fangjun.,Shen, Liangliang.,Chen, Jinghua.,...&Shen, Jian-Ren.(2017).Isolation and characterization of PSI-LHCI super-complex and their sub-complexes from a red alga Cyanidioschyzon merolae.PHOTOSYNTHESIS RESEARCH,133(1-3),201-214.
MLA	Tian, Lirong,et al."Isolation and characterization of PSI-LHCI super-complex and their sub-complexes from a red alga Cyanidioschyzon merolae".PHOTOSYNTHESIS RESEARCH 133.1-3(2017):201-214.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Ye-2017-Differential（342KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文