Differential expression of a WRKY gene between wild and cultivated soybeans correlates to seed size

doi:10.3897/phytokeys.82.13172

	Differential expression of a WRKY gene between wild and cultivated soybeans correlates to seed size
	Gu, Yongzhe 1; Li, Wei 2; Jiang, Hongwei 3; Wang, Yan ; Gao, Huihui 1; Liu, Miao 2; Chen, Qingshan 3; Lai, Yongcai 2; He, Chaoying1
	2017
发表期刊	JOURNAL OF EXPERIMENTAL BOTANY
ISSN	0022-0957
卷号	68 期号:11 页码:2717-2729
摘要	Soybean (Glycine max) probably originated from the wild soybean (Glycine soja). Glycine max has a significantly larger seed size, but the underlying genomic changes are largely unknown. Candidate regulatory genes were preliminarily proposed by data co-localizing RNA sequencing with the quantitative loci (QTLs) for seed size. The soybean gene locus SoyWRKY15a and its orthologous genes from G. max (GmWRKY15a) and G. soja (GsWRKY15a) were analyzed in detail. The coding sequences were nearly identical between the two orthologs, but GmWRKY15a was significantly more highly expressed than GsWRKY15a. Four haplotypes (H1-H4) were found and they varied in the size of a CT-core microsatellite locus in the 5'-untranslated region of this gene. H1 (with six CT-repeats) was the only allelic version found in G. max, while H3 (with five CT-repeats) was the dominant G. soja allele. Differential expression of this gene in soybean pods was correlated with CT-repeat variation, and manipulation of the CT copy number altered the reporter gene expression, suggesting a regulatory role for the simple sequence repeats. Seed weight of wild soybeans harboring H1 was significantly greater than that of soybeans having haplotypes H2, H3, or H4, and seed weight was correlated with gene expression, suggesting the influence of GsWRKY15a in controlling seed size. However, the seed size might be refractory to increased SoyWRKY15a expression in cultivated soybeans. The evolutionary significance of SoyWRKY15a variation in soybean seed domestication is discussed.
关键词	CT-rich regulatory motif domestication expression variation seed size soybean WRKY gene
学科领域	Plant Sciences
DOI	10.3897/phytokeys.82.13172
收录类别	SCI
语种	英语
WOS关键词	TRANSCRIPTION FACTOR ; GLYCINE-MAX ; ARTIFICIAL SELECTION ; CROP DOMESTICATION ; MOLECULAR-BASIS ; ARABIDOPSIS ; EVOLUTION ; PROMOTER ; RESISTANCE ; REVEALS
WOS记录号	WOS:000404967300003
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1460-2431
资助机构	Chinese Academy of SciencesChinese Academy of Sciences [XDA08010105] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31525003]
作者邮箱	chaoying@ibcas.ac.cn
作品OA属性	Green Submitted, gold, Green Published
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22103
专题	系统与进化植物学国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Heilongjiang Acad Agr Sci, Crop Tillage & Cultivat Inst, Harbin 150086, Heilongjiang, Peoples R China 4.Northeast Agr Univ, Coll Agr, Harbin 150030, Heilongjiang, Peoples R China
推荐引用方式 GB/T 7714	Gu, Yongzhe,Li, Wei,Jiang, Hongwei,et al. Differential expression of a WRKY gene between wild and cultivated soybeans correlates to seed size[J]. JOURNAL OF EXPERIMENTAL BOTANY,2017,68(11):2717-2729.
APA	Gu, Yongzhe.,Li, Wei.,Jiang, Hongwei.,Wang, Yan.,Gao, Huihui.,...&He, Chaoying.(2017).Differential expression of a WRKY gene between wild and cultivated soybeans correlates to seed size.JOURNAL OF EXPERIMENTAL BOTANY,68(11),2717-2729.
MLA	Gu, Yongzhe,et al."Differential expression of a WRKY gene between wild and cultivated soybeans correlates to seed size".JOURNAL OF EXPERIMENTAL BOTANY 68.11(2017):2717-2729.

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