Wheat ROP proteins modulate defense response through lignin metabolism

doi:10.1016/j.molp.2016.12.006

	Wheat ROP proteins modulate defense response through lignin metabolism
	Ma, Qing-Hu ; Zhu, Hai-Hao ; Han, Jia-Qi
	2017
发表期刊	PLANT SCIENCE
ISSN	0168-9452
卷号	262 页码:32-38
摘要	ROP is a subfamily of small GTP-binding proteins that uniquely exist in plants. It acts as versatile molecular switches that regulate various developmental processes. Some ROP proteins are also reported to affect defense responses, although their exact mechanism is not fully understood. Herein, ROP members in wheat were mined; the functions of three wheat ROP proteins were studied. RT-PCR results showed that the expression of TaRacl was rapidly and strongly induced after leaf rust infection. TaRacl interacted with TaCCR in yeast-hybridization assay. The overexpression of TaRacl in tobacco promoted CCR and CAD gene expression, increased the total lignin content and sinapyl lignin proportion, and then enhanced resistance to tobacco black shank and bacterial wilt diseases. In contrast, TaRac3 and TaRac4 did not show to interact with TaCCR. Furthermore, the over expression of TaRac3 and TaRac4 did not increase lignin gene expression and lignin accumulation either. Unlike TaRacl, the overexpression of TaRac3 increased susceptibility to both black shank and bacterial wilt pathogens, while overexpression of TaRac4 showed no effect on disease resistance but promoted the root growth in tobacco seedling. These data collectively suggest that TaRacl in Group II is mainly involved in regulating lignin metabolism which, in turn, responsible for the observed roles in pathogen resistance. TaRac3 and TaRac4 have the minor roles in defense response but may act on regulation in plant developmental processes. These results shed light on the complexity and diverse function of ROP in plant defense pathway.
关键词	ROP proteins Lignin metabolism Phytophthora parasitica Pseudomonas solanacearum Disease resistance Wheat
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1016/j.molp.2016.12.006
收录类别	SCI
语种	英语
WOS关键词	SMALL GTPASE RAC ; RICE INNATE IMMUNITY ; NADPH OXIDASE ; MEDICAGO-TRUNCATULA ; DISEASE RESISTANCE ; SCLEROTINIA-SCLEROTIORUM ; ARABIDOPSIS-THALIANA ; ROP/RAC GTPASE ; POWDERY MILDEW ; RAC/ROP GTPASE
WOS记录号	WOS:000396358200015
出版者	ELSEVIER IRELAND LTD
文献子类	Article
出版地	CLARE
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31370336] ; Natural Science Foundation of BeijingBeijing Natural Science Foundation [5122024]
作者邮箱	mqh@ibcas.ac.cn
作品OA属性	Bronze
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22126
专题	中科院北方资源植物重点实验室
作者单位	1.[Ma, Qing-Hu 2.Zhu, Hai-Hao 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Ma, Qing-Hu,Zhu, Hai-Hao,Han, Jia-Qi. Wheat ROP proteins modulate defense response through lignin metabolism[J]. PLANT SCIENCE,2017,262:32-38.
APA	Ma, Qing-Hu,Zhu, Hai-Hao,&Han, Jia-Qi.(2017).Wheat ROP proteins modulate defense response through lignin metabolism.PLANT SCIENCE,262,32-38.
MLA	Ma, Qing-Hu,et al."Wheat ROP proteins modulate defense response through lignin metabolism".PLANT SCIENCE 262(2017):32-38.

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