Duplication and Whorl-Specific-Down Regulation of the Obligate AP3-PI Heterodimer Genes Explain the Origin of Paeonia lactiflora Plants with Spontaneous Corolla Mutation

doi:10.1038/ismej.2016.180

	Duplication and Whorl-Specific-Down Regulation of the Obligate AP3-PI Heterodimer Genes Explain the Origin of Paeonia lactiflora Plants with Spontaneous Corolla Mutation
	Gong, Pichang ; Ao, Xiang 1; Liu, Gaixiu 2; Cheng, Fangyun 1; He, Chaoying3
	2017
发表期刊	PLANT AND CELL PHYSIOLOGY
ISSN	0032-0781
卷号	58 期号:3 页码:411-425
摘要	Herbaceous peony (Paeonia lactiflora) is a globally important ornamental plant. Spontaneous floral mutations occur frequently during cultivation, and are selected as a way to release new cultivars, but the underlying evolutionary developmental genetics remain largely elusive. Here, we investigated a collection of spontaneous corolla mutational plants (SCMPs) whose other floral organs were virtually unaffected. Unlike the corolla in normal plants (NPs) that withered soon after fertilization, the transformed corolla (petals) in SCMPs was greenish and persistent similar to the calyx (sepals). Epidermal cellular morphology of the SCMP corolla was also similar to that of calyx cells, further suggesting a sepaloid corolla in SCMPs. Ten floral MADS-box genes from these Paeonia plants were comparatively characterized with respect to sequence and expression. Codogenic sequence variation of these MADS-box genes was not linked to corolla changes in SCMPs. However, we found that both APETALA3 (AP3) and PISTILLATA (PI) lineages of B-class MADS-box genes were duplicated, and subsequent selective expression alterations of these genes were closely associated with the origin of SCMPs. AP3-PI obligate heterodimerization, essential for organ identity of corolla and stamens, was robustly detected. However, selective down-regulation of these duplicated genes might result in a reduction of this obligate heterodimer concentration in a corolla-specific manner, leading to the sepaloid corolla in SCMPs, thus representing a new sepaloid corolla model taking advantage of gene duplication. Our work suggests that modifying floral MADS-box genes could facilitate the breeding of novel cultivars with distinct floral morphology in ornamental plants, and also provides new insights into the functional evolution of the MADS-box genes in plants.
关键词	Corolla development Expressional variation Functional evolution MADS-box gene Protein-protein interaction Paeonia lactiflora
学科领域	Environmental Sciences & Ecology ; Microbiology
DOI	10.1038/ismej.2016.180
收录类别	SCI
语种	英语
WOS关键词	MADS-BOX GENES ; FLORAL ORGAN IDENTITY ; HOMEOTIC GENE ; FLOWER DEVELOPMENT ; FUNCTIONAL DIVERGENCE ; PROTEIN-INTERACTION ; PETAL IDENTITY ; DNA-BINDING ; ABC MODEL ; B GENES
WOS记录号	WOS:000397281200007
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1471-9053
资助机构	National High Technology Research and Development ProgramNational High Technology Research and Development Program of China [2011AA100207] ; CAS/SAFEA International Partner Program for Creative Research Teams of 'Systematic and Evolutionary Botany'
作者邮箱	chengfy8@263.net ; chaoying@ibcas.ac.cn
作品OA属性	Green Published, Bronze
引用统计	被引频次：46[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22153
专题	系统与进化植物学国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 2.Landscape Architecture Coll Beijing Forestry Univ, Natl Flower Engn Res Ctr, Beijing 100083, Peoples R China 3.Luoyang Natl Peony Garden, Mangshan Town, Luoyang 471011, Peoples R China 4.Univ Chinese Acad Sci, Yuquan Rd 19, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Gong, Pichang,Ao, Xiang,Liu, Gaixiu,et al. Duplication and Whorl-Specific-Down Regulation of the Obligate AP3-PI Heterodimer Genes Explain the Origin of Paeonia lactiflora Plants with Spontaneous Corolla Mutation[J]. PLANT AND CELL PHYSIOLOGY,2017,58(3):411-425.
APA	Gong, Pichang,Ao, Xiang,Liu, Gaixiu,Cheng, Fangyun,&He, Chaoying.(2017).Duplication and Whorl-Specific-Down Regulation of the Obligate AP3-PI Heterodimer Genes Explain the Origin of Paeonia lactiflora Plants with Spontaneous Corolla Mutation.PLANT AND CELL PHYSIOLOGY,58(3),411-425.
MLA	Gong, Pichang,et al."Duplication and Whorl-Specific-Down Regulation of the Obligate AP3-PI Heterodimer Genes Explain the Origin of Paeonia lactiflora Plants with Spontaneous Corolla Mutation".PLANT AND CELL PHYSIOLOGY 58.3(2017):411-425.

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