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| Overexpression of a type-I isopentenyl pyrophosphate isomerase of Artemisia annua in the cytosol leads to high arteannuinB production and artemisinin increase | |
| Ma, Dongming1; Li, Gui; Alejos-Gonzalez, Fatima; Zhu, Yue; Xue, Zhen2; Wang, Aimin2; Zhang, Hui2; Li, Xing3; Ye, Hechun2; Wang, Hong4; Liu, Benye5; Xie, De-Yu | |
| 2017 | |
| 发表期刊 | PLANT JOURNAL
 |
| ISSN | 0960-7412 |
| 卷号 | 91期号:3页码:466-479 |
| 摘要 | We recently characterized a gene-terpene network that is associated with artemisinin biosynthesis in self-pollinated (SP) Artemisia annua, an effective antimalarial plant. We hypothesize that an alteration of gene expression in the network may improve the production of artemisinin and its precursors. In this study, we cloned an isopentenyl pyrophosphate isomerase (IPPI) cDNA, AaIPPI1, from Artemisia annua (Aa). The full-length cDNA encodes a type-I IPPI containing a plastid transit peptide (PTP) at its amino terminus. After the removal of the PTP, the recombinant truncated AaIPPI1 isomerized isopentenyl pyrophosphate (IPP) to dimethyl allyl pyrophosphate (DMAPP) and vice versa. The steady-state equilibrium ratio of IPP/DMAPP in the enzymatic reactions was approximately 1:7. The truncated AaIPPI1 was overexpressed in the cytosol of the SP A.annua variety. The leaves of transgenic plants produced approximately 4% arteannuinB (gg(-1), dry weight, dw) and 0.17-0.25% artemisinin (gg(-1), dw), the levels of which were significantly higher than those in the leaves of wild-type plants. In addition, transgenic plants showed an increase in artemisinic acid production of more than 1% (gg(-1), dw). In contrast, isoprene formation was significantly reduced in transgenic plants. These results provide evidence that overexpression of AaIPPI1 in the cytosol can lead to metabolic alterations of terpenoid biosynthesis, and show that these transgenic plants have the potential to yield high production levels of arteannuinB as a new precursor source for artemisinin. |
| 关键词 | Artemisia annua artemisinin arteannuinB artemisinicacid isopentenyl pyrophosphate isomerase isoprene |
| 学科领域 | Environmental Sciences & Ecology ; Geology ; Meteorology & Atmospheric Sciences |
| DOI | 10.1002/2016GB005557 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | FARNESYL DIPHOSPHATE SYNTHASE ; ISOPRENOID BIOSYNTHESIS ; MOLECULAR-CLONING ; ESCHERICHIA-COLI ; AMORPHA-4,11-DIENE SYNTHASE ; ANTIMALARIAL-DRUG ; QINGHAOSU ARTEMISININ ; MEVALONATE PATHWAY ; PHOSPHATE-PATHWAY ; DRIED LEAVES |
| WOS记录号 | WOS:000405103200005 |
| 出版者 | WILEY |
| 文献子类 | Article |
| 出版地 | HOBOKEN |
| EISSN | 1365-313X |
| 资助机构 | North Carolina Biotechnology Center [550031, 2009-MRG-1117] ; CALS Dean's Enrichment Grants Program, College of Agriculture and Life Science, North Carolina State University, Raleigh, USA ; Knowledge Innovation Program of the Chinese Academy of Sciences, Beijing, P.R. China [KSCX2-SW-329] |
| 作者邮箱 | dxie@ncsu.edu |
| 作品OA属性 | Green Published |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/22172 |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.North Carolina State Univ, Dept Plant & Microbial Biol, Raleigh, NC 27695 USA 2.Guangzhou Univ Chinese Med, Res Ctr Chinese Herbal Resource Sci & Engn, Guangzhou, Guangdong, Peoples R China 3.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China 6.Tech Univ Carolo Wilhelmina Braunschweig, Inst Pharmaceut Biol, Braunschweig, Germany |
| 推荐引用方式 GB/T 7714 | Ma, Dongming,Li, Gui,Alejos-Gonzalez, Fatima,et al. Overexpression of a type-I isopentenyl pyrophosphate isomerase of Artemisia annua in the cytosol leads to high arteannuinB production and artemisinin increase[J]. PLANT JOURNAL,2017,91(3):466-479. |
| APA | Ma, Dongming.,Li, Gui.,Alejos-Gonzalez, Fatima.,Zhu, Yue.,Xue, Zhen.,...&Xie, De-Yu.(2017).Overexpression of a type-I isopentenyl pyrophosphate isomerase of Artemisia annua in the cytosol leads to high arteannuinB production and artemisinin increase.PLANT JOURNAL,91(3),466-479. |
| MLA | Ma, Dongming,et al."Overexpression of a type-I isopentenyl pyrophosphate isomerase of Artemisia annua in the cytosol leads to high arteannuinB production and artemisinin increase".PLANT JOURNAL 91.3(2017):466-479. |
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