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Quantification of near-attomole gibberellins in floral organs dissected from a single Arabidopsis thaliana flower
Li, Dongmei1; Guo, Zhenpeng1; Liu, Cuimei1; Li, Jincheng2; Xu, Wenzhong3; Chen, Yi1,4
2017
发表期刊PLANT JOURNAL
ISSN0960-7412
卷号91期号:3页码:547-557
摘要There remains a methodological bottleneck in the quantification of ultra-trace plant hormones in very tiny plant organs at fresh weights below a milligram. The challenge becomes even more serious in the determination of endogenous gibberellins (GAs), which are a class of compounds that are difficult to separate and detect. Herein, a quantification method using ultra-high-performance liquid chromatography-tandem mass spectrometry was developed, combined with a derivatization technique in which GAs react with N-(3-dimethylaminopropyl)-N-ethylcarbodiimide in ethanol. The method was validated as capable of determining GAs in floral organs (about 80-250g) - pistil, stamens, petals, sepals and receptacle - which were dissected from only one flower of Arabidopsis thaliana. Substantially different abundance patterns of GAs were measured from the floral organs at floral stages 13, 14 and 15 along the non-13-hydroxylation pathway and the early 13-hydroxylation pathway in plants. This allows sub-flower-level insights into how GAs affect floral development. The method exhibited excellent limit of detection and limit of quantification down to 5.41 and 18.0 attomole, respectively, and offered a fairly wide linear range from 0.01 to 25 femtomole with linear coefficients above 0.9961. The precision of the method was evaluated with relative standard deviations below 10.6% for intra-day and 11.4% for inter-day assays, and recoveries ranged from 64.0% to 107%.
关键词gibberellins UHPLC-ESI-MS MS tiny fresh plant organ quantification Arabidopsis thaliana flower
学科领域Plant Sciences
DOI10.1016/j.plaphy.2017.04.010
收录类别SCI
语种英语
WOS关键词CHROMATOGRAPHY-MASS SPECTROMETRY ; PLANT HORMONE ANALYSIS ; ACIDIC PHYTOHORMONES ; SIGNALING GENES ; SHOOT APEX ; FRUIT-SET ; BIOSYNTHESIS ; GROWTH ; DERIVATIZATION ; L.
WOS记录号WOS:000404424200026
出版者WILEY
文献子类Article
出版地HOBOKEN
EISSN1365-313X
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21235007, 21475136, 21621062] ; Chinese Academy of SciencesChinese Academy of Sciences [QYZDJ-SSW-SLH034]
作者邮箱guozhenp@iccas.ac.cn ; chenyi@iccas.ac.cn
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/22190
专题中科院北方资源植物重点实验室
作者单位1.Chinese Acad Sci, Inst Chem, CAS Res Educ Ctr Excellence Mol Sci, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Fishery Sci, Beijing 100141, Peoples R China
4.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
5.Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
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GB/T 7714
Li, Dongmei,Guo, Zhenpeng,Liu, Cuimei,et al. Quantification of near-attomole gibberellins in floral organs dissected from a single Arabidopsis thaliana flower[J]. PLANT JOURNAL,2017,91(3):547-557.
APA Li, Dongmei,Guo, Zhenpeng,Liu, Cuimei,Li, Jincheng,Xu, Wenzhong,&Chen, Yi.(2017).Quantification of near-attomole gibberellins in floral organs dissected from a single Arabidopsis thaliana flower.PLANT JOURNAL,91(3),547-557.
MLA Li, Dongmei,et al."Quantification of near-attomole gibberellins in floral organs dissected from a single Arabidopsis thaliana flower".PLANT JOURNAL 91.3(2017):547-557.
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