Involvement of three putative glucosyltransferases from the UGT72 family in flavonol glucoside/rhamnoside biosynthesis in Lotus japonicus seeds

doi:10.1016/j.biocon.2017.05.029

	Involvement of three putative glucosyltransferases from the UGT72 family in flavonol glucoside/rhamnoside biosynthesis in Lotus japonicus seeds
	Yin, Qinggang 1; Shen, Guoan ; Chang, Zhenzhan 2; Tang, Yuhong 3; Gao, Hongwen 4; Pang, Yongzhen
	2017
发表期刊	JOURNAL OF EXPERIMENTAL BOTANY
ISSN	0022-0957
卷号	68 期号:3 页码:594-609
摘要	Flavonols are one of the largest groups of flavonoids that confer benefits for the health of plants and animals. Flavonol glycosides are the predominant flavonoids present in the model legume Lotus japonicus. The molecular mechanisms underlying the biosynthesis of flavonol glycosides as yet remain unknown in L. japonicus. In the present study, we identified a total of 188 UDP-glycosyltransferases (UGTs) in L. japonicus by genome-wide searching. Notably, 12 UGTs from the UGT72 family were distributed widely among L. japonicus chromosomes, expressed in all tissues, and showed different docking scores in an in silico bioinformatics docking analysis. Further enzymatic assays showed that five recombinant UGTs (UGT72AD1, UGT72AF1, UGT72AH1, UGT72V3, and UGT72Z2) exhibit activity toward flavonol, flavone, and isoflavone aglycones. In particular, UGT72AD1, UGT72AH1, and UGT72Z2 are flavonol-specific UGTs with different kinetic properties. In addition, the overexpression of UGT72AD1 and UGT72Z2 led to increased accumulation of flavonol rhamnosides in L. japonicus and Arabidopsis thaliana. Moreover, the increase of kaempferol 3-O-rhamnoside-7-O-rhamnoside in transgenic A. thaliana inhibited root growth as compared with the wild-type control. These results highlight the significance of the UGT72 family in flavonol glycosylation and the role of flavonol rhamnosides in plant growth.
关键词	Enzymatic activity flavonols Lotus japonicus seed UDP-glycosyltransferase UGT72 family
学科领域	Biodiversity & Conservation ; Environmental Sciences & Ecology
DOI	10.1016/j.biocon.2017.05.029
收录类别	SCI
语种	英语
WOS关键词	UDP-GLYCOSYLTRANSFERASES ; FUNCTIONAL-CHARACTERIZATION ; AUXIN TRANSPORT ; IDENTIFICATION ; GENES ; LEGUME ; EXPRESSION ; DIFFERENTIATION ; TRANSCRIPTOME ; GLYCOSYLATION
WOS记录号	WOS:000404709100001
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1460-2431
资助机构	Major State Basic Research Development ProgramNational Basic Research Program of China [2014CB138703] ; International S&T Cooperation Program of China [2015DFR30570] ; Hundred Talents Program of the Chinese Academy of SciencesChinese Academy of Sciences [39391503-7]
作者邮箱	yzhpang@ibcas.ac.cn
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22200
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Plant Resources, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Peking Univ, Sch Basic Med Sci, Dept Biophys, Beijing 100191, Peoples R China 4.Samuel Roberts Noble Fdn Inc, Ardmore, OK 73042 USA 5.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Yin, Qinggang,Shen, Guoan,Chang, Zhenzhan,et al. Involvement of three putative glucosyltransferases from the UGT72 family in flavonol glucoside/rhamnoside biosynthesis in Lotus japonicus seeds[J]. JOURNAL OF EXPERIMENTAL BOTANY,2017,68(3):594-609.
APA	Yin, Qinggang,Shen, Guoan,Chang, Zhenzhan,Tang, Yuhong,Gao, Hongwen,&Pang, Yongzhen.(2017).Involvement of three putative glucosyltransferases from the UGT72 family in flavonol glucoside/rhamnoside biosynthesis in Lotus japonicus seeds.JOURNAL OF EXPERIMENTAL BOTANY,68(3),594-609.
MLA	Yin, Qinggang,et al."Involvement of three putative glucosyltransferases from the UGT72 family in flavonol glucoside/rhamnoside biosynthesis in Lotus japonicus seeds".JOURNAL OF EXPERIMENTAL BOTANY 68.3(2017):594-609.

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