The F-box Protein KIB1 Mediates Brassinosteroid-Induced Inactivation and Degradation of GSK3-like Kinases in Arabidopsis

doi:10.3389/fpls.2017.01028

	The F-box Protein KIB1 Mediates Brassinosteroid-Induced Inactivation and Degradation of GSK3-like Kinases in Arabidopsis
	Zhu, Jia-Ying ; Li, Yuyao ; Cao, Dong-Mei ; Yang, Hongjuan 1; Oh, Eunkyoo 3; Bi, Yang 4; Zhu, Shengwei1 ; Wang, Zhi-Yong
	2017
发表期刊	MOLECULAR CELL
ISSN	1097-2765
卷号	66 期号:5 页码:648+
摘要	The glycogen synthase kinase-3 (GSK3) family kinases are central cellular regulators highly conserved in all eukaryotes. In Arabidopsis, the GSK3-like kinase BIN2 phosphorylates a range of proteins to control broad developmental processes, and BIN2 is degraded through unknown mechanism upon receptor kinase-mediated brassinosteroid (BR) signaling. Here we identify KIB1 as an F-box E3 ubiquitin ligase that promotes the degradation of BIN2 while blocking its substrate access. Loss-of-function mutations of KIB1 and its homologs abolished BR-induced BIN2 degradation and caused severe BR-insensitive phenotypes. KIB1 directly interacted with BIN2 in a BR-dependent manner and promoted BIN2 ubiquitination in vitro. Expression of an F-box-truncated KIB1 caused BIN2 accumulation but dephosphorylation of its substrate BZR1 and activation of BR responses because KIB1 blocked BIN2 binding to BZR1. Our study demonstrates that KIB1 plays an essential role in BR signaling by inhibiting BIN2 through dual mechanisms of blocking substrate access and promoting degradation.
学科领域	Plant Sciences
DOI	10.3389/fpls.2017.01028
收录类别	SCI
语种	英语
WOS关键词	PLANT-GROWTH REGULATION ; SIGNAL-TRANSDUCTION ; RECEPTOR KINASE ; UBIQUITIN LIGASE ; GENE-EXPRESSION ; IMAGE-ANALYSIS ; BETA-CATENIN ; BRI1 ; THALIANA ; BZR1
WOS记录号	WOS:000403487300001
出版者	CELL PRESS
文献子类	Article
出版地	CAMBRIDGE
EISSN	1097-4164
资助机构	NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01GM066258] ; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [R01GM066258] Funding Source: NIH RePORTER
作者邮箱	zywang24@stanford.edu
作品OA属性	Green Published, gold
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22208
专题	中科院植物分子生理学重点实验室
作者单位	1.Carnegie Inst Sci, Dept Plant Biol, 290 Panama St, Stanford, CA 94305 USA 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Shanxi Acad Agr Sci, Inst Hort, Taiyuan 030031, Peoples R China 4.Chonnam Natl Univ, Dept Bioenergy Sci & Technol, Gwangju 61186, South Korea 5.Stanford Univ, Dept Biol, Stanford, CA 94305 USA
推荐引用方式 GB/T 7714	Zhu, Jia-Ying,Li, Yuyao,Cao, Dong-Mei,et al. The F-box Protein KIB1 Mediates Brassinosteroid-Induced Inactivation and Degradation of GSK3-like Kinases in Arabidopsis[J]. MOLECULAR CELL,2017,66(5):648+.
APA	Zhu, Jia-Ying.,Li, Yuyao.,Cao, Dong-Mei.,Yang, Hongjuan.,Oh, Eunkyoo.,...&Wang, Zhi-Yong.(2017).The F-box Protein KIB1 Mediates Brassinosteroid-Induced Inactivation and Degradation of GSK3-like Kinases in Arabidopsis.MOLECULAR CELL,66(5),648+.
MLA	Zhu, Jia-Ying,et al."The F-box Protein KIB1 Mediates Brassinosteroid-Induced Inactivation and Degradation of GSK3-like Kinases in Arabidopsis".MOLECULAR CELL 66.5(2017):648+.

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