Transcriptional Gene Silencing Maintained by OTS1 SUMO Protease Requires a DNA-Dependent Polymerase V-Dependent Pathway

doi:10.5194/bg-14-3083-2017

	Transcriptional Gene Silencing Maintained by OTS1 SUMO Protease Requires a DNA-Dependent Polymerase V-Dependent Pathway
	Liu, Lei ; Yan, Xiaojing ; Kong, Xiangxiong 1; Zhao, Yiqiang ; Gong, Zhizhong ; Jin, Jing Bo 1; Guo, Yan
	2017
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
卷号	173 期号:1 页码:655-667
摘要	The expression of genes with aberrant structure is prevented at both the transcriptional and posttranscriptional regulation levels. Aberrant gene silencing at the posttranscriptional level is well studied; however, it is not well understood how aberrant genes are silenced at the transcriptional level. In this study, through genetic screening a transgenic report line that harbors an aberrant gene (35S-LUC, lacking 39-untranslated region [39-UTR]) and lacks luciferase (LUC) activity, we identify that the small ubiquitin-like modifier (SUMO) protease OTS1 gene is required for maintaining the silence of the reporter 35S-LUC and an endogenous mutator-like element MULE-F19G14 at the transcriptional level, which requires DNA-dependent RNA polymerase (Pol) V and DDR complex, but not Pol IV. The increased transcripts in ots1 mutants are terminated by the 39-UTRs of downstream genes. In addition to ots1 mutations, mutations in several known or putative SUMO proteases and two SUMO E3 ligases, SIZ1 and MMS21, have similar effects on this silencing regulation. Taken together, our results reveal that the enzymes involved in the SUMOylation process restrain aberrant gene transcription by using a downstream gene 39-UTR, and this regulation requires a functional Pol V-dependent pathway in Arabidopsis (Arabidopsis thaliana).
学科领域	Environmental Sciences & Ecology ; Geology
DOI	10.5194/bg-14-3083-2017
收录类别	SCI
语种	英语
WOS关键词	E3 LIGASE SIZ1 ; ARABIDOPSIS-THALIANA ; SALICYLIC-ACID ; OVERLY TOLERANT ; MESSENGER-RNA ; POL IV ; METHYLATION ; SUMOYLATION ; UBIQUITIN ; PLANTS
WOS记录号	WOS:000404404800001
出版者	AMER SOC PLANT BIOLOGISTS
文献子类	Article
出版地	ROCKVILLE
EISSN	1532-2548
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31430012] ; Foundation for Innovative Research Group of the National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31421062]
作者邮箱	guoyan@cau.edu.cn
作品OA属性	Green Submitted, gold
引用统计	被引频次：35[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22209
专题	中科院植物分子生理学重点实验室
作者单位	1.China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Liu, Lei,Yan, Xiaojing,Kong, Xiangxiong,et al. Transcriptional Gene Silencing Maintained by OTS1 SUMO Protease Requires a DNA-Dependent Polymerase V-Dependent Pathway[J]. PLANT PHYSIOLOGY,2017,173(1):655-667.
APA	Liu, Lei.,Yan, Xiaojing.,Kong, Xiangxiong.,Zhao, Yiqiang.,Gong, Zhizhong.,...&Guo, Yan.(2017).Transcriptional Gene Silencing Maintained by OTS1 SUMO Protease Requires a DNA-Dependent Polymerase V-Dependent Pathway.PLANT PHYSIOLOGY,173(1),655-667.
MLA	Liu, Lei,et al."Transcriptional Gene Silencing Maintained by OTS1 SUMO Protease Requires a DNA-Dependent Polymerase V-Dependent Pathway".PLANT PHYSIOLOGY 173.1(2017):655-667.

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