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| ZYGOTE-ARREST 3 that encodes the tRNA ligase is essential for zygote division in Arabidopsis | |
Yang, Ke-Jin; Guo, Lei; Hou, Xiu-Li; Gong, Hua-Qin; Liu, Chun-Ming
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| 2017 | |
| Source Publication | JOURNAL OF INTEGRATIVE PLANT BIOLOGY
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| ISSN | 1672-9072 |
| Volume | 59Issue:9Pages:680-692 |
| Abstract | In sexual organisms, division of the zygote initiates a new life cycle. Although several genes involved in zygote division are known in plants, how the zygote is activated to start embryogenesis has remained elusive. Here, we showed that a mutation in ZYGOTE-ARREST 3 (ZYG3) in Arabidopsis led to a tight zygote-lethal phenotype. Map-based cloning revealed that ZYG3 encodes the transfer RNA (tRNA) ligase AtRNL, which is a single-copy gene in the Arabidopsis genome. Expression analyses showed that AtRNL is expressed throughout zygotic embryogenesis, and in meristematic tissues. Using pAtRNL::cAtRNL-sYFP-complemented zyg3/zyg3 plants, we showed that AtRNL is localized exclusively in the cytoplasm, suggesting that tRNA splicing occurs primarily in the cytoplasm. Analyses using partially rescued embryos showed that mutation in AtRNL compromised splicing of intron-containing tRNA. Mutations of two tRNA endonuclease genes, SEN1 and SEN2, also led to a zygote-lethal phenotype. These results together suggest that tRNA splicing is critical for initiating zygote division in Arabidopsis. |
| Subject Area | Forestry |
| DOI | 10.1007/s11676-016-0364-5 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | UNFOLDED PROTEIN RESPONSE ; SPLICING ENDONUCLEASE ; MESSENGER-RNA ; SACCHAROMYCES-CEREVISIAE ; FUNCTIONAL DOMAINS ; EMBRYOGENESIS ; THALIANA ; KINASE ; REPAIR ; IDENTIFICATION |
| WOS ID | WOS:000404234900016 |
| Publisher | WILEY |
| Special Issue | SI |
| Subtype | Article |
| Publication Place | HOBOKEN |
| EISSN | 1744-7909 |
| Funding Organization | National Basic Research Program of China from MOST [2014CB943401] |
| Corresponding Author Email | cmliu@ibcas.ac.cn |
| OA | Green Submitted |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/22215 |
| Collection | 中科院植物分子生理学重点实验室 |
| Affiliation | 1.[Yang, Ke-Jin 2.Guo, Lei 3.Hou, Xiu-Li 4.Gong, Hua-Qin 5.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 7.Nanyang Normal Univ, Sch Life Sci & Technol, Nanyang 473061, Peoples R China 8.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China |
| Recommended Citation GB/T 7714 | Yang, Ke-Jin,Guo, Lei,Hou, Xiu-Li,et al. ZYGOTE-ARREST 3 that encodes the tRNA ligase is essential for zygote division in Arabidopsis[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2017,59(9):680-692. |
| APA | Yang, Ke-Jin,Guo, Lei,Hou, Xiu-Li,Gong, Hua-Qin,&Liu, Chun-Ming.(2017).ZYGOTE-ARREST 3 that encodes the tRNA ligase is essential for zygote division in Arabidopsis.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,59(9),680-692. |
| MLA | Yang, Ke-Jin,et al."ZYGOTE-ARREST 3 that encodes the tRNA ligase is essential for zygote division in Arabidopsis".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 59.9(2017):680-692. |
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| File Name/Size | DocType | Version | Access | License | ||
| Zhuang-2017-Differen（975KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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