9-cis-Neoxanthin in Light Harvesting Complexes of Photosystem II Regulates the Binding of Violaxanthin and Xanthophyll Cycle

doi:10.3389/fpls.2017.02154

IB-CAS > 中科院光生物学重点实验室

	9-cis-Neoxanthin in Light Harvesting Complexes of Photosystem II Regulates the Binding of Violaxanthin and Xanthophyll Cycle
	Wang, Ke 1; Tu, Wenfeng ; Liu, Cheng ; Rao, Yan ; Gao, Zhimin 2; Yang, Chunhong
	2017
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
卷号	174 期号:1 页码:86-96
摘要	The light-harvesting chlorophyll a/b complex of photosystem II (LHCII) is able to switch to multiple functions under different light conditions (i.e. harvesting solar energy for photosynthesis and dissipating excess excitation energy for photoprotection). The role of the different carotenoids bound to LHCII in regulating the structure and function of the complex is a long-lasting question in photosynthesis research. 9-cis-Neoxanthin (Nx) is one of the important carotenoids, which can only be found in the LHCIIs. High-resolution structural analysis of LHCII shows that Nx is located between different monomeric LHCIIs, with one side protruding into the lipid membrane. In this study, the various functional significances of this unique feature of Nx binding in LHCII are studied with the in vitro reconstituted LHCIIs both with and without Nx and the native complexes isolated either from wild-type Arabidopsis (Arabidopsis thaliana) or from its mutant aba4-3 lacking Nx. Our results reveal that the binding of Nx affects the binding affinity of violaxanthin (Vx) to LHCII significantly. In the absence of Nx, Vx has a much higher binding affinity to trimeric LHCII. The strong coordination between Nx and Vx at the interfaces of adjacent monomers of LHCII plays an important role both in operating the xanthophyll cycle and in the transient modulation of nonphotochemical quenching.
学科领域	Plant Sciences
DOI	10.3389/fpls.2017.02154
收录类别	SCI
语种	英语
WOS关键词	CHLOROPHYLL-A/B-COMPLEX ; MAJOR ANTENNA COMPLEX ; HIGHER-PLANTS ; DE-EPOXIDASE ; ANGSTROM RESOLUTION ; FUNCTIONAL-ANALYSIS ; ENERGY-DISSIPATION ; NEOXANTHIN-BINDING ; CRYSTAL-STRUCTURE ; STRUCTURAL BASIS
WOS记录号	WOS:000418035000001
出版者	AMER SOC PLANT BIOLOGISTS
文献子类	Article
出版地	ROCKVILLE
EISSN	1532-2548
资助机构	Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDB17030100] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31370275, 31570236, 31600192, 31370588]
作者邮箱	yangch@ibcas.ac.cn
作品OA属性	gold, Green Published
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22244
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Int Ctr Bamboo & Rattan, Key Lab Sci & Technol Bamboo & Rattan, Beijing 100102, Peoples R China
推荐引用方式 GB/T 7714	Wang, Ke,Tu, Wenfeng,Liu, Cheng,et al. 9-cis-Neoxanthin in Light Harvesting Complexes of Photosystem II Regulates the Binding of Violaxanthin and Xanthophyll Cycle[J]. PLANT PHYSIOLOGY,2017,174(1):86-96.
APA	Wang, Ke,Tu, Wenfeng,Liu, Cheng,Rao, Yan,Gao, Zhimin,&Yang, Chunhong.(2017).9-cis-Neoxanthin in Light Harvesting Complexes of Photosystem II Regulates the Binding of Violaxanthin and Xanthophyll Cycle.PLANT PHYSIOLOGY,174(1),86-96.
MLA	Wang, Ke,et al."9-cis-Neoxanthin in Light Harvesting Complexes of Photosystem II Regulates the Binding of Violaxanthin and Xanthophyll Cycle".PLANT PHYSIOLOGY 174.1(2017):86-96.

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