Organizational Innovation of Apical Actin Filaments Drives Rapid Pollen Tube Growth and Turning

doi:10.1038/s41598-017-07517-w

	Organizational Innovation of Apical Actin Filaments Drives Rapid Pollen Tube Growth and Turning
	Qu, Xiaolu ; Zhang, Ruihui ; Zhang, Meng 1; Diao, Min 1; Xue, Yongbiao 2,3; Huang, Shanjin 1
	2017
发表期刊	MOLECULAR PLANT
ISSN	1674-2052
卷号	10 期号:7 页码:930-947
摘要	Polarized tip growth is a fundamental cellular process in many eukaryotes. In this study, we examined the dynamic restructuring of the actin cytoskeleton and its relationship to vesicle transport during pollen tip growth in Arabidopsis. We found that actin filaments originating from the apical membrane form a specialized structure consisting of longitudinally aligned actin bundles at the cortex and inner cytoplasmic filaments with a distinct distribution. Using actin-based pharmacological treatments and genetic mutants in combination with FRAP (fluorescence recovery after photobleaching) technology to visualize the transport of vesicles within the growth domain of pollen tubes, we demonstrated that cortical actin filaments facilitate tip-ward vesicle transport. We also discovered that the inner apical actin filaments prevent backward movement of vesicles, thus ensuring that sufficient vesicles accumulate at the pollen tube tip to support the rapid growth of the pollen tube. The combinatorial effect of cortical and internal apical actin filaments perfectly explains the generation of the inverted V'' cone-shaped vesicle distribution pattern at the pollen tube tip. When pollen tubes turn, apical actin filaments at the facing side undergo depolymerization and repolymerization to reorient the apical actin structure toward the new growth direction. This actin restructuring precedes vesicle accumulation and changes in tube morphology. Thus, our study provides new insights into the functional relationship between actin dynamics and vesicle transport during rapid and directional pollen tube growth.
关键词	pollen tube clear zone apical actin structure actin dynamics vesicle trafficking myosin
学科领域	Science & Technology - Other Topics
DOI	10.1038/s41598-017-07517-w
收录类别	SCI
语种	英语
WOS关键词	GROWING PLANT-CELLS ; TIP GROWTH ; POLARIZED GROWTH ; ENDOMEMBRANE TRAFFICKING ; FLUORESCENCE RECOVERY ; LILIUM-LONGIFLORUM ; BINDING PROTEINS ; ARABIDOPSIS ; CYTOSKELETON ; DYNAMICS
WOS记录号	WOS:000406980800015
出版者	CELL PRESS
文献子类	Article
出版地	CAMBRIDGE
EISSN	1752-9867
资助机构	Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2013CB945100] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31671390, 31471266] ; Tsinghua-Peking Joint Center for Life Sciences ; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2015M571028]
作者邮箱	sjhuang@tsinghua.edu.cn
作品OA属性	gold, Green Published
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22254
专题	中科院植物分子生理学重点实验室
作者单位	1.Tsinghua Univ, Sch Life Sci, Ctr Plant Biol, Beijing 100084, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Beijing Inst Genom, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Qu, Xiaolu,Zhang, Ruihui,Zhang, Meng,et al. Organizational Innovation of Apical Actin Filaments Drives Rapid Pollen Tube Growth and Turning[J]. MOLECULAR PLANT,2017,10(7):930-947.
APA	Qu, Xiaolu,Zhang, Ruihui,Zhang, Meng,Diao, Min,Xue, Yongbiao,&Huang, Shanjin.(2017).Organizational Innovation of Apical Actin Filaments Drives Rapid Pollen Tube Growth and Turning.MOLECULAR PLANT,10(7),930-947.
MLA	Qu, Xiaolu,et al."Organizational Innovation of Apical Actin Filaments Drives Rapid Pollen Tube Growth and Turning".MOLECULAR PLANT 10.7(2017):930-947.

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