DEVELOPMENT OF MICROSATELLITE MARKERS FOR CYPRIPEDIUM TIBETICUM (ORCHIDACEAE) AND THEIR APPLICABILITY TO TWO RELATED SPECIES

doi:10.3390/f8090344

	DEVELOPMENT OF MICROSATELLITE MARKERS FOR CYPRIPEDIUM TIBETICUM (ORCHIDACEAE) AND THEIR APPLICABILITY TO TWO RELATED SPECIES
	Li, Jing ; Luo, Yibo1 ; Xu, Lingling
	2017
发表期刊	APPLICATIONS IN PLANT SCIENCES
ISSN	2168-0450
卷号	5 期号:12
摘要	Premise of the study: To investigate genetic diversity and structure, microsatellite markers were developed and characterized for Cypripedium tibeticum, an endangered orchid species, and their utility tested in two relatives: C. flavum and C. bardolphianum. Methods and Results: Candidate microsatellite loci were isolated based on next-generation sequencing. We identified 20 polymorphic loci with di- or trinucleotide repeats in three populations of C. tibeticum. Genotyping results showed the total number of alleles per locus varied from two to 21, and the observed and expected heterozygosity ranged from 0.261 to 0.967 and 0.441 to 0.960, respectively. Cross-species amplification showed most of the markers were applicable to C. flavum and C. bardolphianum. Conclusions: The 20 polymorphic microsatellite markers developed in this study are useful tools for studying the conservation genetics and phylogeography of C. tibeticum and its related species.
关键词	Cypripedium tibeticum genetic diversity microsatellites Orchidaceae
学科领域	Forestry
DOI	10.3390/f8090344
收录类别	SCI
语种	英语
WOS记录号	WOS:000411523700042
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [NSFC 31300366] ; Natural Science Foundation of Shaanxi ProvinceNatural Science Foundation of Shaanxi Province [2015JQ3091] ; Natural Science Foundation of Xi'an Science and Technology Bureau [CXY1531WL28] ; Scientific Research Foundation of the Education Department of Shaanxi [15JK2146]
作者邮箱	missilexll@163.com
作品OA属性	gold, Green Submitted
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22281
专题	系统与进化植物学国家重点实验室
作者单位	1.Xian Univ, Sch Biol & Environm Engn, 1 Keji Six Rd, Xian 710065, Shaanxi, Peoples R China 2.Chinese Acad Sci, State Key Lab Systemat & Evolutionary Bot, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Li, Jing,Luo, Yibo,Xu, Lingling. DEVELOPMENT OF MICROSATELLITE MARKERS FOR CYPRIPEDIUM TIBETICUM (ORCHIDACEAE) AND THEIR APPLICABILITY TO TWO RELATED SPECIES[J]. APPLICATIONS IN PLANT SCIENCES,2017,5(12).
APA	Li, Jing,Luo, Yibo,&Xu, Lingling.(2017).DEVELOPMENT OF MICROSATELLITE MARKERS FOR CYPRIPEDIUM TIBETICUM (ORCHIDACEAE) AND THEIR APPLICABILITY TO TWO RELATED SPECIES.APPLICATIONS IN PLANT SCIENCES,5(12).
MLA	Li, Jing,et al."DEVELOPMENT OF MICROSATELLITE MARKERS FOR CYPRIPEDIUM TIBETICUM (ORCHIDACEAE) AND THEIR APPLICABILITY TO TWO RELATED SPECIES".APPLICATIONS IN PLANT SCIENCES 5.12(2017).

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