Transcriptome Analyses Reveal Candidate Pod Shattering-Associated Genes Involved in the Pod Ventral Sutures of Common Vetch (Vicia sativa L.)

doi:10.1016/j.biocon.2016.05.022

	Transcriptome Analyses Reveal Candidate Pod Shattering-Associated Genes Involved in the Pod Ventral Sutures of Common Vetch (Vicia sativa L.)
	Dong, Rui ; Dong, Deke ; Luo, Dong ; Zhou, Qiang ; Chai, Xutian ; Zhang, Jiyu ; Xie, Wengang ; Liu, Wenxian ; Dong, Yang1 ; Wang, Yanrong ; Liu, Zhipeng
	2017
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	8
摘要	The seed dispersion caused by pod shattering is a form of propagation used by many wild species. Loss of seeds from pod shattering is frequent in the common vetch (Vicia sativa L.), an important self-pollinating annual forage legume. However, pod shattering is one of the most important defects that limits the reproduction of the vetch in the field and the usage as a leguminous forage crop. To better understand the vetch pod shattering mechanism, we used high-throughput RNA sequencing to assess the global changes in the transcriptomes of the pod ventral sutures of shattering-susceptible and shattering-resistant vetch accessions screened from 541 vetch germplasms. A total of 1,285 significantly differentially expressed unigenes (DEGs) were detected, including 575 up-regulated unigenes and 710 down-regulated unigenes. Analyses of Gene Ontology and KEGG metabolic enrichment pathways of 1,285 DEGs indicated that 22 DEGs encoding cell wall modifications and hydrolases associated with pod shattering were highly expressed in shattering-susceptible accessions. These genes were mainly enriched in hydrolase activity, cytoplasm, and carbohydrate metabolic process systems. These cell wall modifications and hydrolases genes included beta-glucosidase and endo-polygalacturonase, which work together to break down the glycosidic bonds of pectin and cellulose, and to promote the dissolution and disappearance of the cell wall in the ventral suture of the pod and make the pod more susceptible to shattering. We demonstrated the differences in gene transcription levels between the shattering-susceptible and shattering-resistant vetch accessions for the first time and our results provided valuable information for the identifying and characterizing of pod shattering regulation networks in vetch. This information may facilitate the future identification of pod shattering-related genes and their underlying molecular mechanisms in the common vetch.
关键词	Vicia sativa L. transcriptome pod ventral suture pod shattering cell wall hydrolases
学科领域	Biodiversity & Conservation ; Environmental Sciences & Ecology
DOI	10.1016/j.biocon.2016.05.022
收录类别	SCI
语种	英语
WOS关键词	RNA-SEQ DATA ; REFERENCE GENOME ; DEHISCENCE ZONE ; BRASSICA-NAPUS ; SUBSP SATIVA ; SSR MARKERS ; MAJOR QTL ; POLYGALACTURONASE ; RESISTANCE ; FRUIT
WOS记录号	WOS:000404709100013
出版者	FRONTIERS MEDIA SA
文献子类	Article
出版地	LAUSANNE
资助机构	National Basic Research Program of ChinaNational Basic Research Program of China [2014CB138704] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31672476]
作者邮箱	yrwang@lzu.edu.cn ; lzp@lzu.edu.cn
引用统计	被引频次：32[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22297
专题	系统与进化植物学国家重点实验室
作者单位	1.Lanzhou Univ, Coll Pastoral Agr Sci & Technol, State Key Lab Grassland Agroecosyst, Lanzhou, Peoples R China 2.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Dong, Rui,Dong, Deke,Luo, Dong,et al. Transcriptome Analyses Reveal Candidate Pod Shattering-Associated Genes Involved in the Pod Ventral Sutures of Common Vetch (Vicia sativa L.)[J]. FRONTIERS IN PLANT SCIENCE,2017,8.
APA	Dong, Rui.,Dong, Deke.,Luo, Dong.,Zhou, Qiang.,Chai, Xutian.,...&Liu, Zhipeng.(2017).Transcriptome Analyses Reveal Candidate Pod Shattering-Associated Genes Involved in the Pod Ventral Sutures of Common Vetch (Vicia sativa L.).FRONTIERS IN PLANT SCIENCE,8.
MLA	Dong, Rui,et al."Transcriptome Analyses Reveal Candidate Pod Shattering-Associated Genes Involved in the Pod Ventral Sutures of Common Vetch (Vicia sativa L.)".FRONTIERS IN PLANT SCIENCE 8(2017).

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