Evaluation of DNA barcodes in Codonopsis (Campanulaceae) and in some large angiosperm plant genera

doi:10.1371/journal.pone.0175727

	Evaluation of DNA barcodes in Codonopsis (Campanulaceae) and in some large angiosperm plant genera
	Wang, De-Yi ; Wang, Qiang ; Wang, Ying-Li ; Xiang, Xiao-Guo ; Huang, Lu-Qi ; Jin, Xiao-Hua
	2017
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	12 期号:2
摘要	DNA barcoding is expected to be one of the most promising tools in biological taxonomy. However, there have been no agreements on which core barcode should be used in plants, especially in species-rich genera with wide geographical distributions. To evaluate their discriminatory power in large genera, four of the most widely used DNA barcodes, including three plastid regions (matK, rbcL, trnH-psbA) and nuclear internal transcribed spacer (nrITS), were tested in seven species-rich genera (Ficus, Pedicularis, Rhodiola, Rhododendron, Viburnum, Dendrobium and Lysimachia) and a moderate size genus, Codonopsis. All of the sequences from the aforementioned seven large genera were downloaded from NCBI. The related barcodes for Codonopsis were newly generated in this study. Genetics distances, DNA barcoding gaps and phylogenetic trees of the four single barcodes and their combinations were calculated and compared in the seven genera. As for single barcode, nrITS has the most variable sites, the clearest intra- and inter-specific divergences and the highest discrimination rates in the seven genera. Among the combinations of barcodes, ITS +matK performed better than all the single barcodes in most cases and even the three- and four-loci combinations in the seven genera. Therefore, we recommend ITS+matK as the core barcodes for large plant genera.
学科领域	Science & Technology - Other Topics
DOI	10.1371/journal.pone.0175727
收录类别	SCI
语种	英语
WOS关键词	LAND PLANTS ; IDENTIFICATION ; ORCHIDACEAE ; COMPLEMENTS ; EVOLUTION ; TAXONOMY ; HISTORY ; GENOMES ; PROMISE ; SPACER
WOS记录号	WOS:000399875200040
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
作者邮箱	huangluqi01@163.com ; xiaohuajin@ibcas.ac.cn
作品OA属性	Green Accepted, gold, Green Published, Green Submitted
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22302
专题	系统与进化植物学国家重点实验室
作者单位	1.[Wang, De-Yi 2.Wang, Qiang 3.Xiang, Xiao-Guo 4.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China 6.Baotou Med Coll, Baotou City, Inner Mongolia, Peoples R China 7.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, Beijing, Peoples R China 8.Chinese Acad Sci, SEABRI, Southeast Asia Biodivers Res Inst, Mengla, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Wang, De-Yi,Wang, Qiang,Wang, Ying-Li,et al. Evaluation of DNA barcodes in Codonopsis (Campanulaceae) and in some large angiosperm plant genera[J]. PLOS ONE,2017,12(2).
APA	Wang, De-Yi,Wang, Qiang,Wang, Ying-Li,Xiang, Xiao-Guo,Huang, Lu-Qi,&Jin, Xiao-Hua.(2017).Evaluation of DNA barcodes in Codonopsis (Campanulaceae) and in some large angiosperm plant genera.PLOS ONE,12(2).
MLA	Wang, De-Yi,et al."Evaluation of DNA barcodes in Codonopsis (Campanulaceae) and in some large angiosperm plant genera".PLOS ONE 12.2(2017).

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