Arabidopsis AMINO ACID PERMEASE1 Contributes to Salt Stress-Induced Proline Uptake from Exogenous Sources

doi:10.1016/j.ppees.2017.08.001

	Arabidopsis AMINO ACID PERMEASE1 Contributes to Salt Stress-Induced Proline Uptake from Exogenous Sources
	Wang, Ting 1; Chen, Ying 1; Zhang, Min ; Chen, Jiugeng ; Liu, Jie 1; Han, Huiling 1; Hua, Xuejun
	2017
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	8
摘要	Stress-induced proline accumulation in plants is thought to result primarily from enhanced proline biosynthesis and decreased proline degradation. To identify regulatory components involved in proline transport, we screened for Arabidopsis thaliana T-DNA mutants with enhanced tolerance to toxic levels of exogenous proline (45 mM). We isolated the proline resistant 1-1 (pre1-1) mutant and map-based cloning identified PRE1 as AMINO ACID PERMEASE1 (AAP1, At1g58360), which encodes a plasma membrane-localized amino acid permease. AAP1 expression is induced by salt stress and abscisic acid, but not by proline. In pre1-1 mutants, a 19-nucleotide deletion in the AAP1 coding region produced a premature stop codon. When grown on proline-containing medium, pre1-1 mutants accumulated significantly less proline than did the wild type. Under salt stress, proline uptake decreased significantly in pre1-1 mutants. By contrast, proline uptake increased significantly in the wild type. These results suggest that AAP1 functions in the increase of proline uptake during salt stress. In addition, proline uptake promotes salt tolerance in Arabidopsis seedlings. We conclude that plants can increase proline accumulation by AtAAP1-mediated proline uptake from exogenous source, which help to improve the salt tolerance of seedlings.
关键词	Arabidopsis thaliana amino acid permease positional cloning proline accumulation proline transport salt stress
学科领域	Plant Sciences ; Environmental Sciences & Ecology
DOI	10.1016/j.ppees.2017.08.001
收录类别	SCI
语种	英语
WOS关键词	DELTA(1)-PYRROLINE-5-CARBOXYLATE SYNTHETASE ; OSMOTIC-STRESS ; TRANSGENIC PLANTS ; GLYCINE BETAINE ; GENE-EXPRESSION ; WATER-STRESS ; TRANSPORTERS ; TOLERANCE ; METABOLISM ; THALIANA
WOS记录号	WOS:000417791100008
出版者	FRONTIERS MEDIA SA
文献子类	Article
出版地	LAUSANNE
资助机构	National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31271308]
作者邮箱	xjhua@ibcas.ac.cn
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22343
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources & Beijing Bot Garden, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Ting,Chen, Ying,Zhang, Min,et al. Arabidopsis AMINO ACID PERMEASE1 Contributes to Salt Stress-Induced Proline Uptake from Exogenous Sources[J]. FRONTIERS IN PLANT SCIENCE,2017,8.
APA	Wang, Ting.,Chen, Ying.,Zhang, Min.,Chen, Jiugeng.,Liu, Jie.,...&Hua, Xuejun.(2017).Arabidopsis AMINO ACID PERMEASE1 Contributes to Salt Stress-Induced Proline Uptake from Exogenous Sources.FRONTIERS IN PLANT SCIENCE,8.
MLA	Wang, Ting,et al."Arabidopsis AMINO ACID PERMEASE1 Contributes to Salt Stress-Induced Proline Uptake from Exogenous Sources".FRONTIERS IN PLANT SCIENCE 8(2017).

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