Knowledge Management System Of Institute Of Botany,CAS
| Over-Expression of Arabidopsis EDT1 Gene Confers Drought Tolerance in Alfalfa (Medicago sativa L.) | |
| Zheng, Guangshun1,2,3; Fan, Cunying1,3; Di, Shaokang1; Wang, Xuemin2; Xiang, Chengbin4; Pang, Yongzhen1,2 | |
| 2017 | |
| 发表期刊 | FRONTIERS IN PLANT SCIENCE
 |
| ISSN | 1664-462X |
| 卷号 | 8 |
| 摘要 | Alfalfa (Medicago sativa L.) is an important legume forage crop with great economic value. However, as the growth of alfalfa is seriously affected by an inadequate supply of water, drought is probably the major abiotic environmental factor that most severely affects alfalfa production worldwide. In an effort to enhance alfalfa drought tolerance, we transformed the Arabidopsis Enhanced Drought Tolerance 1 (AtEDT1) gene into alfalfa via Agrobacterium-mediated transformation. Compared with wild type plants, drought stress treatment resulted in higher survival rates and biomass, but reduced water loss rates in the transgenic plants. Furthermore, transgenic alfalfa plants had increased stomatal size, but reduced stomatal density, and these stomatal changes contributed greatly to reduced water loss from leaves. Importantly, transgenic alfalfa plants exhibited larger root systems with larger root lengths, root weight, and root diameters than wild type plants. The transgenic alfalfa plants had reduced membrane permeability and malondialdehyde content, but higher soluble sugar and proline content, higher superoxide dismutase activity, higher chlorophyll content, enhanced expression of drought-responsive genes, as compared with wild type plants. Notably, transgenic alfalfa plants grew better in a 2-year field trial and showed enhanced growth performance with increased biomass yield. All of our morphological, physiological, and molecular analyses demonstrated that the ectopic expression of AtEDT1 improved growth and enhanced drought tolerance in alfalfa. Our study provides alfalfa germplasm for use in forage improvement programs, and may help to increase alfalfa production in arid lands. |
| 关键词 | alfalfa drought tolerance AtEDT1 gene transgenic alfalfa over-expression |
| 学科领域 | Geology ; Paleontology |
| DOI | 10.1016/j.jop.2017.08.005 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | NAC TRANSCRIPTION FACTOR ; CO-TRANSFORMING BAR ; ACTIVATED EXPRESSION ; ENHANCED RESISTANCE ; STRESS TOLERANCE ; SALT TOLERANCE ; OVEREXPRESSION ; YIELD ; COTTON ; ATEDT1/HDG11 |
| WOS记录号 | WOS:000418500000005 |
| 出版者 | FRONTIERS MEDIA SA |
| 文献子类 | Article |
| 出版地 | LAUSANNE |
| 资助机构 | Major State Basic Research and Development Program of ChinaNational Basic Research Program of China [2014CB138703] ; International S&T Cooperation Program of China [2015DFR30570-3] |
| 作者邮箱 | pangyongzhen@caas.cn |
| 作品OA属性 | gold |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/22344 |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing, Peoples R China 3.Chinese Acad Agr Sci, Inst Anim Sci, Beijing, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Univ Sci & Technol China, Sch Life Sci, Hefei, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Guangshun,Fan, Cunying,Di, Shaokang,et al. Over-Expression of Arabidopsis EDT1 Gene Confers Drought Tolerance in Alfalfa (Medicago sativa L.)[J]. FRONTIERS IN PLANT SCIENCE,2017,8. |
| APA | Zheng, Guangshun,Fan, Cunying,Di, Shaokang,Wang, Xuemin,Xiang, Chengbin,&Pang, Yongzhen.(2017).Over-Expression of Arabidopsis EDT1 Gene Confers Drought Tolerance in Alfalfa (Medicago sativa L.).FRONTIERS IN PLANT SCIENCE,8. |
| MLA | Zheng, Guangshun,et al."Over-Expression of Arabidopsis EDT1 Gene Confers Drought Tolerance in Alfalfa (Medicago sativa L.)".FRONTIERS IN PLANT SCIENCE 8(2017). |
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