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| Characterization of UGT716A1 as a Multi-substrate UDP: Flavonoid Glucosyltransferase Gene in Ginkgo biloba | |
| Su, Xiaojia1,2,3; Shen, Guoan1; Di, Shaokang1; Dixon, Richard A.; Pang, Yongzhen1,2 | |
| 2017 | |
| Source Publication | FRONTIERS IN PLANT SCIENCE
 |
| ISSN | 1664-462X |
| Volume | 8 |
| Abstract | Ginkgo biloba L., a living fossil and medicinal plant, is a well-known rich source of bioactive flavonoids. The molecular mechanism underlying the biosynthesis of flavonoid glucosides, the predominant flavonoids in G. biloba, remains unclear. To better understand flavonoid glucosylation in G. biloba, we generated a transcriptomic dataset of G. biloba leaf tissue by high-throughput RNA sequencing. We identified 25 putative UDP-glycosyltransferase (UGT) unigenes that are potentially involved in the flavonoid glycosylation. Among them, we successfully isolated and expressed eight UGT genes in Escherichia coli, and found that recombinant UGT716A1 protein was active toward broad range of flavonoid/phenylpropanoid substrates. In particular, we discovered the first recombinant UGT protein, UGT716A1 from G. biloba, possessing unique activity toward flavanol gallates that have been extensively documented to have significant bioactivity relating to human health. UGT716A1 expression level paralleled the flavonoid distribution pattern in G. biloba. Ectopic over-expression of UGT716A1 in Arabidopsis thaliana led to increased accumulation of several flavonol glucosides. Identification and comparison of the in vitro enzymatic activity of UGT716A1 homologs revealed a UGT from the primitive land species Physcomitrella patens also showed broader substrate spectrum than those from higher plants A. thaliana, Vitis vinifera, and Medicago truncatula. The characterization of UGT716A1 from G. biloba bridges a gap in the evolutionary history of UGTs in gymnosperms. We also discuss the implication of UGT716A1 for biosynthesis, evolution, and bioengineering of diverse glucosylated flavonoids. |
| Keyword | Ginkgo biloba flavonoids UGT716A1 multi-substrate UGT EGCG flavanol gallate glycosides |
| Subject Area | Science & Technology - Other Topics |
| DOI | 10.1038/nature23897 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | SECONDARY METABOLISM GLYCOSYLTRANSFERASES ; CHEMICAL-ANALYSIS ; QUALITY-CONTROL ; PLANT ; ARABIDOPSIS ; BIOSYNTHESIS ; TEA ; GENOME ; FAMILY ; IDENTIFICATION |
| WOS ID | WOS:000411381300034 |
| Publisher | FRONTIERS MEDIA SA |
| Subtype | Article |
| Publication Place | LAUSANNE |
| Funding Organization | Major State Basic Research and Development ProgramNational Basic Research Program of China [2013CB127002] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31670305] ; Hundred Talents Program of the Chinese Academy of SciencesChinese Academy of Sciences [39391503-7] |
| Corresponding Author Email | shenguoan@ibcas.ac.cn ; pangyongzhen@caas.cn |
| OA | Green Published, hybrid |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/22345 |
| Collection | 中科院北方资源植物重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Key Lab Plant Resources, Inst Bot, Beijing, Peoples R China 2.Chinese Acad Sci, Beijing Bot Garden, Inst Bot, Beijing, Peoples R China 3.Chinese Acad Agr Sci, Inst Anim Sci, Beijing, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Dixon, Richard A.] Univ North Texas, BioDiscovery Inst, Denton, TX 76203 USA 6.Dixon, Richard A.] Univ North Texas, Dept Biol Sci, Denton, TX 76203 USA 7.Dixon, Richard A.] Beijing Forestry Univ, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Su, Xiaojia,Shen, Guoan,Di, Shaokang,et al. Characterization of UGT716A1 as a Multi-substrate UDP: Flavonoid Glucosyltransferase Gene in Ginkgo biloba[J]. FRONTIERS IN PLANT SCIENCE,2017,8. |
| APA | Su, Xiaojia,Shen, Guoan,Di, Shaokang,Dixon, Richard A.,&Pang, Yongzhen.(2017).Characterization of UGT716A1 as a Multi-substrate UDP: Flavonoid Glucosyltransferase Gene in Ginkgo biloba.FRONTIERS IN PLANT SCIENCE,8. |
| MLA | Su, Xiaojia,et al."Characterization of UGT716A1 as a Multi-substrate UDP: Flavonoid Glucosyltransferase Gene in Ginkgo biloba".FRONTIERS IN PLANT SCIENCE 8(2017). |
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| File Name/Size | DocType | Version | Access | License | ||
| Zhang-2017-The Apost（3697KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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