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Identification and Functional Divergence Analysis of WOX Gene Family in Paper Mulberry | |
Tang, Feng1; Chen, Naizhi; Zhao, Meiling1; Wang, Yucheng; He, Ruiping; Peng, Xianjun; Shen, Shihua | |
2017 | |
发表期刊 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES |
卷号 | 18期号:8 |
摘要 | The WOX (WUSCHEL-related homeobox) is a plant-specific transcription factor involved in plant development and stress response. However, few studies have been reported on the WOX gene in woody plants. In this study, 10 BpWOX genes were isolated from paper mulberry by RACE-PCR and categorized into three clades through phylogenetic analysis, ancient, intermediate and WUS clade. Among them, five members had the transcriptional activity detected by yeast one-hybrid and seven were uniquely localized to the nucleus through green fluorescent protein (GFP) observation. The expression patterns of BpWOX genes in different tissues and under diverse treatments were quantified by the qRT-PCR method. Results showed that BpWUS was expressed in the apical bud, stem and root, BpWOX5 and BpWOX7 functioned only in the root tip, and three BpWOXs regulated leaf development redundantly. BpWOX9 and BpWOX10 were induced by indole-3-acetic acid (IAA) or jasmonic acid (JA), while BpWOX2 was repressed by five phytohormones. Interestingly, most BpWOX genes were responsive to the abiotic stress stimuli of drought, salt, cold, and cadmium (CdCl2). Together, our study revealed that BpWOXs were functionally divergent during paper mulberry development and environmental adaptation, which might be related to their evolutionary relationships. Our work will benefit the systematic understanding of the precise function of WOX in plant development and environmental stress responses. |
关键词 | WOX transcription factor paper mulberry development phytohormone stress response |
学科领域 | Plant Sciences |
DOI | 10.11646/phytotaxa.321.2.8 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | HOMEOBOX GENE ; ORGAN DEVELOPMENT ; ROOT DEVELOPMENT ; BLADE OUTGROWTH ; WUSCHEL GENE ; CELL FATE ; ARABIDOPSIS ; SHOOT ; LEAF ; LEAVES |
WOS记录号 | WOS:000410684600008 |
出版者 | MDPI |
文献子类 | Article |
出版地 | BASEL |
EISSN | 1422-0067 |
资助机构 | Poverty Relief Project of the Chinese Academy of Sciences [KFJ-FP-24] ; Huimin Technology Demonstration Project of the National Modern Agricultural Science and Technology Achievements City [Z151100001015008] |
作者邮箱 | tangfeng76@ibcas.ac.cn ; chennaizhi@ibcas.ac.cn ; zml@ibcas.ac.cn ; wangyucheng731@berrygenomics.com ; heruipinging@126.com ; pengxianjun@ibcas.ac.cn ; shshen@ibcas.ac.cn |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/22346 |
专题 | 中科院北方资源植物重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Tang, Feng,Chen, Naizhi,Zhao, Meiling,et al. Identification and Functional Divergence Analysis of WOX Gene Family in Paper Mulberry[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2017,18(8). |
APA | Tang, Feng.,Chen, Naizhi.,Zhao, Meiling.,Wang, Yucheng.,He, Ruiping.,...&Shen, Shihua.(2017).Identification and Functional Divergence Analysis of WOX Gene Family in Paper Mulberry.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,18(8). |
MLA | Tang, Feng,et al."Identification and Functional Divergence Analysis of WOX Gene Family in Paper Mulberry".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 18.8(2017). |
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phytotaxa.321.2.8.pd(1913KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 |
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