Knowledge Management System Of Institute Of Botany,CAS
An efficient method for transgenic callus induction from Vitis amurensis petiole | |
Zhao, Tingting1; Wang, Zemin; Su, Lingye1,2; Sun, Xiaoming2; Cheng, Jun2; Zhang, Langlang1; Karungo, Sospeter Karanja1; Han, Yuepeng; Li, Shaohua2; Xin, Haiping | |
2017 | |
发表期刊 | PLOS ONE |
ISSN | 1932-6203 |
卷号 | 12期号:6 |
摘要 | Transformation is the main platform for genetic improvement and gene function studies in plants. However, the established somatic embryo transformation system for grapevines is time-consuming and has low efficiency, which limits its utilization in functional genomics research. Vitis amurensis is a wild Vitis species with remarkable cold tolerance. The lack of an efficient genetic transformation system for it has significantly hindered the functional identification of cold stress related genes in the species. Herein, an efficient method was established to produce transformed calli of V. amurensis. Segments of petioles from micro-propagated plantlets of V. amurensis exhibited better capacity to differentiate calli than leaf-discs and stem segments, and thus was chosen as target tissue for Agrobacterium-mediated transformation. Both neomycin phosphotransferase II (NPTII) and enhanced green fluorescent protein (eGFP) genes were used for simultaneous selection of transgenic calli based on kanamycin resistance and eGFP fluorescence. Several parameters affecting the transformation efficiency were optimized including the concentration of kanamycin, Agrobacterium stains, bacterial densities, infection treatments and co-cultivation time. The transgenic callus lines were verified by checking the integration of NPTII gene into calli genomes, the expression of eGFP gene and the fluorescence of eGFP. Up to 20% of the petiole segments produced transformed calli after 2 months of cultivation. This efficient transformation system will facilitate the functional analysis of agronomic characteristics and related genes not only in V. amurensis but also in other grapevine species. |
学科领域 | Plant Sciences |
DOI | 10.1111/jse.12283 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | AGROBACTERIUM-MEDIATED TRANSFORMATION ; SOMATIC EMBRYOGENESIS ; PLANT-REGENERATION ; VINIFERA L. ; GENETIC-TRANSFORMATION ; TRANSCRIPTION FACTOR ; SHOOT ORGANOGENESIS ; MUSCADINE GRAPE ; COLD-STRESS ; ARABIDOPSIS |
WOS记录号 | WOS:000410947300002 |
出版者 | PUBLIC LIBRARY SCIENCE |
文献子类 | Article |
出版地 | SAN FRANCISCO |
资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471857, 31672132] ; Youth Innovation Promotion Association of CAS [2015281] |
作者邮箱 | xinhaiping215@hotmail.com |
作品OA属性 | Green Published, Bronze |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/22348 |
专题 | 中科院北方资源植物重点实验室 |
作者单位 | 1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Inst Bot, CAS Key Lab Plant Resources, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | Zhao, Tingting,Wang, Zemin,Su, Lingye,et al. An efficient method for transgenic callus induction from Vitis amurensis petiole[J]. PLOS ONE,2017,12(6). |
APA | Zhao, Tingting.,Wang, Zemin.,Su, Lingye.,Sun, Xiaoming.,Cheng, Jun.,...&Xin, Haiping.(2017).An efficient method for transgenic callus induction from Vitis amurensis petiole.PLOS ONE,12(6). |
MLA | Zhao, Tingting,et al."An efficient method for transgenic callus induction from Vitis amurensis petiole".PLOS ONE 12.6(2017). |
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