IB-CAS  > 中科院北方资源植物重点实验室
An efficient method for transgenic callus induction from Vitis amurensis petiole
Zhao, Tingting1; Wang, Zemin; Su, Lingye1,2; Sun, Xiaoming2; Cheng, Jun2; Zhang, Langlang1; Karungo, Sospeter Karanja1; Han, Yuepeng; Li, Shaohua2; Xin, Haiping
2017
发表期刊PLOS ONE
ISSN1932-6203
卷号12期号:6
摘要Transformation is the main platform for genetic improvement and gene function studies in plants. However, the established somatic embryo transformation system for grapevines is time-consuming and has low efficiency, which limits its utilization in functional genomics research. Vitis amurensis is a wild Vitis species with remarkable cold tolerance. The lack of an efficient genetic transformation system for it has significantly hindered the functional identification of cold stress related genes in the species. Herein, an efficient method was established to produce transformed calli of V. amurensis. Segments of petioles from micro-propagated plantlets of V. amurensis exhibited better capacity to differentiate calli than leaf-discs and stem segments, and thus was chosen as target tissue for Agrobacterium-mediated transformation. Both neomycin phosphotransferase II (NPTII) and enhanced green fluorescent protein (eGFP) genes were used for simultaneous selection of transgenic calli based on kanamycin resistance and eGFP fluorescence. Several parameters affecting the transformation efficiency were optimized including the concentration of kanamycin, Agrobacterium stains, bacterial densities, infection treatments and co-cultivation time. The transgenic callus lines were verified by checking the integration of NPTII gene into calli genomes, the expression of eGFP gene and the fluorescence of eGFP. Up to 20% of the petiole segments produced transformed calli after 2 months of cultivation. This efficient transformation system will facilitate the functional analysis of agronomic characteristics and related genes not only in V. amurensis but also in other grapevine species.
学科领域Plant Sciences
DOI10.1111/jse.12283
收录类别SCI
语种英语
WOS关键词AGROBACTERIUM-MEDIATED TRANSFORMATION ; SOMATIC EMBRYOGENESIS ; PLANT-REGENERATION ; VINIFERA L. ; GENETIC-TRANSFORMATION ; TRANSCRIPTION FACTOR ; SHOOT ORGANOGENESIS ; MUSCADINE GRAPE ; COLD-STRESS ; ARABIDOPSIS
WOS记录号WOS:000410947300002
出版者PUBLIC LIBRARY SCIENCE
文献子类Article
出版地SAN FRANCISCO
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471857, 31672132] ; Youth Innovation Promotion Association of CAS [2015281]
作者邮箱xinhaiping215@hotmail.com
作品OA属性Green Published, Bronze
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/22348
专题中科院北方资源植物重点实验室
作者单位1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Inst Bot, CAS Key Lab Plant Resources, Beijing, Peoples R China
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GB/T 7714
Zhao, Tingting,Wang, Zemin,Su, Lingye,et al. An efficient method for transgenic callus induction from Vitis amurensis petiole[J]. PLOS ONE,2017,12(6).
APA Zhao, Tingting.,Wang, Zemin.,Su, Lingye.,Sun, Xiaoming.,Cheng, Jun.,...&Xin, Haiping.(2017).An efficient method for transgenic callus induction from Vitis amurensis petiole.PLOS ONE,12(6).
MLA Zhao, Tingting,et al."An efficient method for transgenic callus induction from Vitis amurensis petiole".PLOS ONE 12.6(2017).
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