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A Genetic Transformation Method for Cadmium Hyperaccumulator Sedum plumbizincicola and Non-hyperaccumulating Ecotype of Sedum alfredii
Liu, Huan1; Zhao, Haixia1; Wu, Longhua2; Xu, Wenzhong
2017
发表期刊FRONTIERS IN PLANT SCIENCE
ISSN1664-462X
卷号8
摘要The present study demonstrates the development of an Agrobacterium-mediated genetic transformation method for species of the Sedum genus, which includes the Cd/Zn hyperaccumulator Sedum plumbizincicola and the non-hyperaccumulating ecotype of S. alfredii. Multiple shoots were induced from stem nodes of two Sedum plants using Murashige and Skoog (MS) medium containing 0.1 mg/L cytokinin 6-benzyladenine (6-BA) and 1.0 mg/L auxin 1-naphthaleneacetic acid (NAA). The shoot primordia were used as direct targets for Agrobacterium infection. Selection on hygromycin was highly effective in generating Agrobacterium-transformed explants. This callus-free procedure allowed us to obtain transgenic plantlets after rooting hygromycin-resistant shoots on phytohormone-free MS medium containing the antibiotic. The presence and expression of the reporter genes gusA and GFP in transgenic plants were confirmed by a real-time polymerase chain reaction, histochemical GUS assays, and confocal microscopy. This reliable method for genetic transformation of Sedum plants will help us to understand gene functions and the molecular mechanisms underlying Cd hypertolerance and hyperaccumulation in these species.
关键词Agrobacterium tumefaciens genetic transformation cadmium hyperaccumulator multiple shoot buds Sedum alfredii Sedum plumbizincicola
学科领域Plant Sciences
DOI10.1086/692764
收录类别SCI
语种英语
WOS关键词AGROBACTERIUM-MEDIATED TRANSFORMATION ; CRASSULACEAN ACID METABOLISM ; METAL HYPERACCUMULATION ; MOLECULAR-MECHANISMS ; LEAF EXPLANTS ; CD TOLERANCE ; KEY ROLE ; REGENERATION ; PLANTS ; L.
WOS记录号WOS:000407712400005
出版者FRONTIERS MEDIA SA
文献子类Article
出版地LAUSANNE
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41325003, 91117010] ; Knowledge Innovation Programs of CAS [KSCX2-YW-G053]
作者邮箱lhwu@issas.ac.cn ; xuwzh@ibcas.ac.cn
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/22349
专题中科院北方资源植物重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing, Jiangsu, Peoples R China
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GB/T 7714
Liu, Huan,Zhao, Haixia,Wu, Longhua,et al. A Genetic Transformation Method for Cadmium Hyperaccumulator Sedum plumbizincicola and Non-hyperaccumulating Ecotype of Sedum alfredii[J]. FRONTIERS IN PLANT SCIENCE,2017,8.
APA Liu, Huan,Zhao, Haixia,Wu, Longhua,&Xu, Wenzhong.(2017).A Genetic Transformation Method for Cadmium Hyperaccumulator Sedum plumbizincicola and Non-hyperaccumulating Ecotype of Sedum alfredii.FRONTIERS IN PLANT SCIENCE,8.
MLA Liu, Huan,et al."A Genetic Transformation Method for Cadmium Hyperaccumulator Sedum plumbizincicola and Non-hyperaccumulating Ecotype of Sedum alfredii".FRONTIERS IN PLANT SCIENCE 8(2017).
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