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A Genetic Transformation Method for Cadmium Hyperaccumulator Sedum plumbizincicola and Non-hyperaccumulating Ecotype of Sedum alfredii | |
Liu, Huan1; Zhao, Haixia1; Wu, Longhua2; Xu, Wenzhong | |
2017 | |
发表期刊 | FRONTIERS IN PLANT SCIENCE |
ISSN | 1664-462X |
卷号 | 8 |
摘要 | The present study demonstrates the development of an Agrobacterium-mediated genetic transformation method for species of the Sedum genus, which includes the Cd/Zn hyperaccumulator Sedum plumbizincicola and the non-hyperaccumulating ecotype of S. alfredii. Multiple shoots were induced from stem nodes of two Sedum plants using Murashige and Skoog (MS) medium containing 0.1 mg/L cytokinin 6-benzyladenine (6-BA) and 1.0 mg/L auxin 1-naphthaleneacetic acid (NAA). The shoot primordia were used as direct targets for Agrobacterium infection. Selection on hygromycin was highly effective in generating Agrobacterium-transformed explants. This callus-free procedure allowed us to obtain transgenic plantlets after rooting hygromycin-resistant shoots on phytohormone-free MS medium containing the antibiotic. The presence and expression of the reporter genes gusA and GFP in transgenic plants were confirmed by a real-time polymerase chain reaction, histochemical GUS assays, and confocal microscopy. This reliable method for genetic transformation of Sedum plants will help us to understand gene functions and the molecular mechanisms underlying Cd hypertolerance and hyperaccumulation in these species. |
关键词 | Agrobacterium tumefaciens genetic transformation cadmium hyperaccumulator multiple shoot buds Sedum alfredii Sedum plumbizincicola |
学科领域 | Plant Sciences |
DOI | 10.1086/692764 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | AGROBACTERIUM-MEDIATED TRANSFORMATION ; CRASSULACEAN ACID METABOLISM ; METAL HYPERACCUMULATION ; MOLECULAR-MECHANISMS ; LEAF EXPLANTS ; CD TOLERANCE ; KEY ROLE ; REGENERATION ; PLANTS ; L. |
WOS记录号 | WOS:000407712400005 |
出版者 | FRONTIERS MEDIA SA |
文献子类 | Article |
出版地 | LAUSANNE |
资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41325003, 91117010] ; Knowledge Innovation Programs of CAS [KSCX2-YW-G053] |
作者邮箱 | lhwu@issas.ac.cn ; xuwzh@ibcas.ac.cn |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/22349 |
专题 | 中科院北方资源植物重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing, Jiangsu, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Huan,Zhao, Haixia,Wu, Longhua,et al. A Genetic Transformation Method for Cadmium Hyperaccumulator Sedum plumbizincicola and Non-hyperaccumulating Ecotype of Sedum alfredii[J]. FRONTIERS IN PLANT SCIENCE,2017,8. |
APA | Liu, Huan,Zhao, Haixia,Wu, Longhua,&Xu, Wenzhong.(2017).A Genetic Transformation Method for Cadmium Hyperaccumulator Sedum plumbizincicola and Non-hyperaccumulating Ecotype of Sedum alfredii.FRONTIERS IN PLANT SCIENCE,8. |
MLA | Liu, Huan,et al."A Genetic Transformation Method for Cadmium Hyperaccumulator Sedum plumbizincicola and Non-hyperaccumulating Ecotype of Sedum alfredii".FRONTIERS IN PLANT SCIENCE 8(2017). |
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692764.pdf(14513KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 |
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