Identified of a novel cis-element regulating the alternative splicing of LcDREB2

doi:10.1038/hdy.2017.28

	Identified of a novel cis-element regulating the alternative splicing of LcDREB2
	Liu, Zhujiang 1; Yuan, Guangxiao 1; Liu, Shu 1; Jia, Junting 1; Cheng, Liqin ; Qi, Dongmei ; Shen, Shihua; Peng, Xianjun ; Liu, Gongshe
	2017
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	7
摘要	Alternative splicing (AS) is an important gene regulation mechanism in plants. Despite the widespread use of AS in plant gene expression regulation, the identification of the cis-elements involved in the AS mechanism is rarely reported in plants. To explore the regulation mechanism of the AS of LcDREB2, a DREB2 ortholog from Sheepgrass (Leymus chinensis), the genomic sequences of LcDREB2 and its homologs in Poaceae were aligned, and six mutations were introduced in the conserved sequence of LcDREB2. By analyzing the distinct transcript patterns of the LcDREB2 mutants in transgenic Oryza sativa, a novel cis-element that affected the AS of LcDREB2 was identified as Exonic Splicing Enhancer 1 (ESE1). In addition, five serine-arginine rich (SR) proteins were confirmed to interact with ESE1 by electrophoretic mobility shift assay (EMSA). To further explore the expression regulation mechanism of the DREB subfamily, phylogenetic analysis of DREB2 paralogous genes was performed. The results strongly supported the hypothesis that AS is conserved in Poaceae plants and that it is an evolutionary strategy for the regulation of the functional expression of genes. The findings and methods of our study will promote a substantial step forward in understanding of the plant AS regulation mechanism.
学科领域	Environmental Sciences & Ecology ; Evolutionary Biology ; Genetics & Heredity
DOI	10.1038/hdy.2017.28
收录类别	SCI
语种	英语
WOS关键词	RESPONSIVE GENE-EXPRESSION ; ARABIDOPSIS TRANSCRIPTION FACTOR ; PRE-MESSENGER-RNA ; FUNCTIONAL-ANALYSIS ; STRESS TOLERANCE ; BINDING-PROTEIN ; FACTOR DREB2A ; SR PROTEINS ; PLANTS ; LANDSCAPE
WOS记录号	WOS:000407362100007
出版者	NATURE PUBLISHING GROUP
文献子类	Article
出版地	LONDON
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31470411] ; National Basic Research Program of ChinaNational Basic Research Program of China [2014CB138704, 973] ; Conservation of Forage Genetic Resources Porgram [2130135] ; Project of Ningxia Agricultural Comprehensive Development Office [NTKJ-2014-04(1)] ; Project of Science and Technology Service Network Initiative of the Chinese Academy of Sciences [KFJ-EW-STS-119]
作者邮箱	pengxianjun@ibcas.ac.cn ; liugs@ibcas.ac.cn
作品OA属性	Bronze, Green Published
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22351
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Zhujiang,Yuan, Guangxiao,Liu, Shu,et al. Identified of a novel cis-element regulating the alternative splicing of LcDREB2[J]. SCIENTIFIC REPORTS,2017,7.
APA	Liu, Zhujiang.,Yuan, Guangxiao.,Liu, Shu.,Jia, Junting.,Cheng, Liqin.,...&Liu, Gongshe.(2017).Identified of a novel cis-element regulating the alternative splicing of LcDREB2.SCIENTIFIC REPORTS,7.
MLA	Liu, Zhujiang,et al."Identified of a novel cis-element regulating the alternative splicing of LcDREB2".SCIENTIFIC REPORTS 7(2017).

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