Post-transcriptional regulation of fruit ripening and disease resistance in tomato by the vacuolar protease SlVPE3

doi:10.1016/j.gr.2017.06.006

	Post-transcriptional regulation of fruit ripening and disease resistance in tomato by the vacuolar protease SlVPE3
	Wang, Weihao ; Cai, Jianghua 1; Wang, Peiwen 1; Tian, Shiping1 ; Qin, Guozheng
	2017
发表期刊	GENOME BIOLOGY
ISSN	1474-760X
卷号	18
摘要	Background: Proteases represent one of the most abundant classes of enzymes in eukaryotes and are known to play key roles in many biological processes in plants. However, little is known about their functions in fruit ripening and disease resistance, which are unique to flowering plants and required for seed maturation and dispersal. Elucidating the genetic mechanisms of fruit ripening and disease resistance is an important goal given the biological and dietary significance of fruit. Results: Through expression profile analyses of genes encoding tomato (Solanum lycopersicum) cysteine proteases, we identify a number of genes whose expression increases during fruit ripening. RNA interference (RNAi)-mediated repression of SlVPE3, a vacuolar protease gene, results in alterations in fruit pigmentation, lycopene biosynthesis, and ethylene production, suggesting that SlVPE3 is necessary for normal fruit ripening. Surprisingly, the SlVPE3 RNAi fruit are more susceptible to the necrotrophic pathogen Botrytis cinerea. Quantitative proteomic analysis identified 314 proteins that differentially accumulate upon SlVPE3 silencing, including proteins associated with fruit ripening and disease resistance. To identify the direct SlVPE3 targets and mechanisms contributing to fungal pathogen resistance, we perform a screening of SlVPE3-interacting proteins using co-immunoprecipitation coupled with mass spectrometry. We show that SlVPE3 is required for the cleavage of the serine protease inhibitor KTI4, which contributes to resistance against the fungal pathogen B. cinerea. Conclusions: Our findings contribute to elucidating gene regulatory networks and mechanisms that control fruit ripening and disease resistance responses.
关键词	Fruit ripening Disease resistance Protease Vacuolar processing enzyme RNA interference Quantitative proteome Protease inhibitor Tomato
学科领域	Geology
DOI	10.1016/j.gr.2017.06.006
收录类别	SCI
语种	英语
WOS关键词	FLUORESCENT FUSION PROTEINS ; KUNITZ TRYPSIN-INHIBITOR ; PROCESSING ENZYME ; SERINE-PROTEASE ; GENE ; ETHYLENE ; REVEALS ; EXPRESSION ; RIN ; SUSCEPTIBILITY
WOS记录号	WOS:000414383200018
出版者	BMC
文献子类	Article
出版地	LONDON
资助机构	National Basic Research Program of China (973 Program)National Basic Research Program of China [2013CB127103] ; National Natural Science Foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [31172004] ; Youth Innovation Promotion Association CAS
作者邮箱	gzqin@ibcas.ac.cn
作品OA属性	Green Accepted
引用统计	被引频次：86[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22353
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, 20 Nanxincun, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Weihao,Cai, Jianghua,Wang, Peiwen,et al. Post-transcriptional regulation of fruit ripening and disease resistance in tomato by the vacuolar protease SlVPE3[J]. GENOME BIOLOGY,2017,18.
APA	Wang, Weihao,Cai, Jianghua,Wang, Peiwen,Tian, Shiping,&Qin, Guozheng.(2017).Post-transcriptional regulation of fruit ripening and disease resistance in tomato by the vacuolar protease SlVPE3.GENOME BIOLOGY,18.
MLA	Wang, Weihao,et al."Post-transcriptional regulation of fruit ripening and disease resistance in tomato by the vacuolar protease SlVPE3".GENOME BIOLOGY 18(2017).

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