BhbZIP60 from Resurrection Plant Boea hygrometrica Is an mRNA Splicing-Activated Endoplasmic Reticulum Stress Regulator Involved in Drought Tolerance

doi:10.1016/j.palwor.2017.01.006

	BhbZIP60 from Resurrection Plant Boea hygrometrica Is an mRNA Splicing-Activated Endoplasmic Reticulum Stress Regulator Involved in Drought Tolerance
	Wang, Bo 1; Du, Hong 2; Zhang, Zhennan ; Xu, Wenzhong ; Deng, Xin
	2017
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	8
摘要	Adverse environmental conditions cause endoplasmic reticulum (ER) stress in plants. To mitigate ER stress damage, ER associated transcription factors and inositol-requiring enzyme-1 (IRE1)-mediated bZIP60 mRNA splicing are activated in plants. A drought-induced gene, encoding the ortholog of AtbZIP60, was identified in the resurrection plant Boea hygrometrica, termed BhbZIP60. In response to ER stress and dehydration, BhbZIP60 mRNA can be spliced to create a frame shift in the C terminus by the excision of 23b segment in a manner of its ortholog in other plants, thus translocating to the nucleus instead of the cytoplasm. The splicing-activated BhbZIP60 (BhbZIP60S) could function in the same way as its Arabidopsis ortholog by restoring the molecular phenotype of the mutant atbzip60. When overexpressed in Arabidopsis, BhbZIP60S provided transgenic plants with enhanced tolerance to drought, tunicamycin and mannitol stresses with upregulation of the expressions of ER quality control (QC) genes (BiP2, BiP3, CNX1, and sPDI) and abscisic acid (ABA) responsive genes (RD29A, RAB18, and RD17). Furthermore, in the yeast one-hybrid system, BhbZIP60S was capable of interacting with ER stress responsive elements (ERSE and ERSE-II) that exist in the promoters of known ER-QC genes, but not binding to ABA responsive ciselements (ABREs). Our results demonstrated that drought-induced BhbZIP60 may have a function in drought tolerance via the splicing-activated BhbZIP60S to mediate ER-QC by direct binding to the promoters of ER-QC genes. This study evidently demonstrates the involvement of ER-QC in the drought tolerance of Arabidopsis and the desiccation tolerance of the resurrection plant B. hygrometrica.
关键词	Arabidopsis Boea hygrometrica bZIP60 drought tolerance ER-QC mRNA-splicing UPR
学科领域	Paleontology
DOI	10.1016/j.palwor.2017.01.006
收录类别	SCI
语种	英语
WOS关键词	UNFOLDED PROTEIN RESPONSE ; BZIP TRANSCRIPTION FACTOR ; QUALITY-CONTROL ; ER-STRESS ; SIGNAL-TRANSDUCTION ; ABSCISIC-ACID ; ARABIDOPSIS ; GENES ; ABA ; DEHYDRATION
WOS记录号	WOS:000408673600011
出版者	FRONTIERS MEDIA SA
文献子类	Article
出版地	LAUSANNE
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31300230, 31270312, 31470361] ; National Basic Research Program of China (973 Program)National Basic Research Program of China [2012CB114302]
作者邮箱	xuwzh@ibcas.ac.cn ; deng@ibcas.ac.cn
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22354
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Xinjiang Agr Univ, Coll Agr, Urumqi, Peoples R China 3.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Bo,Du, Hong,Zhang, Zhennan,et al. BhbZIP60 from Resurrection Plant Boea hygrometrica Is an mRNA Splicing-Activated Endoplasmic Reticulum Stress Regulator Involved in Drought Tolerance[J]. FRONTIERS IN PLANT SCIENCE,2017,8.
APA	Wang, Bo,Du, Hong,Zhang, Zhennan,Xu, Wenzhong,&Deng, Xin.(2017).BhbZIP60 from Resurrection Plant Boea hygrometrica Is an mRNA Splicing-Activated Endoplasmic Reticulum Stress Regulator Involved in Drought Tolerance.FRONTIERS IN PLANT SCIENCE,8.
MLA	Wang, Bo,et al."BhbZIP60 from Resurrection Plant Boea hygrometrica Is an mRNA Splicing-Activated Endoplasmic Reticulum Stress Regulator Involved in Drought Tolerance".FRONTIERS IN PLANT SCIENCE 8(2017).

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