Biodegradation Mechanisms of Patulin in Candida guilliermondii: An iTRAQ-Based Proteomic Analysis

doi:10.1093/hmg/ddx147

	Biodegradation Mechanisms of Patulin in Candida guilliermondii: An iTRAQ-Based Proteomic Analysis
	Chen, Yong ; Peng, Huai-Min ; Wang, Xiao ; Li, Bo-Qiang ; Long, Man-Yuan ; Tian, Shi-Ping
	2017
发表期刊	TOXINS
ISSN	2072-6651
卷号	9 期号:2
摘要	Patulin, a potent mycotoxin, contaminates fruits and derived products worldwide, and is a serious health concern. Several yeast strains have shown the ability to effectively degrade patulin. However, the mechanisms of its biodegradation still remain unclear at this time. In the present study, biodegradation and involved mechanisms of patulin by an antagonistic yeast Candida guilliermondii were investigated. The results indicated that C. guilliermondii was capable of not only multiplying to a high population in medium containing patulin, but also effectively reducing patulin content in culture medium. Degradation of patulin by C. guilliermondii was dependent on the yeast cell viability, and mainly occurred inside cells. E-ascladiol was the main degradation product of patulin. An iTRAQ-based proteomic analysis revealed that the responses of C. guilliermondii to patulin were complex. A total of 30 differential proteins involved in 10 biological processes were identified, and more than two-thirds of the differential proteins were down-accumulated. Notably, a short-chain dehydrogenase (gi\| 190348612) was markedly induced by patulin at both the protein and mRNA levels. Our findings will provide a foundation to help enable the commercial development of an enzyme formulation for the detoxification of patulin in fruit-derived products.
关键词	contamination detoxification mycotoxins patulin Penicillium yeast
学科领域	Biochemistry & Molecular Biology ; Genetics & Heredity
DOI	10.1093/hmg/ddx147
收录类别	SCI
语种	英语
WOS关键词	BIOLOGICAL-CONTROL ; PENICILLIUM-EXPANSUM ; MYCOTOXIN PATULIN ; GENE-EXPRESSION ; BIOCONTROL ; DETOXIFICATION ; BIOSYNTHESIS ; DEGRADATION ; INSIGHTS ; REVEALS
WOS记录号	WOS:000405102100005
出版者	MDPI
文献子类	Article
出版地	BASEL
资助机构	National Key Research and Development Program of China [2016YFD0400902] ; National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31371863, 31530057] ; Youth Innovation Promotion Association CAS [2015063]
作者邮箱	cy123898@163.com ; huaimin1216@163.com ; wangxiao2@ibcas.ac.cn ; bqli@ibcas.ac.cn ; mlong@uchicago.edu ; tsp@ibcas.ac.cn
作品OA属性	Green Published
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22357
专题	中科院北方资源植物重点实验室
作者单位	1.[Chen, Yong 2.Peng, Huai-Min 3.Wang, Xiao 4.Li, Bo-Qiang 5.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 7.Univ Chicago, Dept Ecol & Evolut, 940 E 57Th St, Chicago, IL 60637 USA
推荐引用方式 GB/T 7714	Chen, Yong,Peng, Huai-Min,Wang, Xiao,et al. Biodegradation Mechanisms of Patulin in Candida guilliermondii: An iTRAQ-Based Proteomic Analysis[J]. TOXINS,2017,9(2).
APA	Chen, Yong,Peng, Huai-Min,Wang, Xiao,Li, Bo-Qiang,Long, Man-Yuan,&Tian, Shi-Ping.(2017).Biodegradation Mechanisms of Patulin in Candida guilliermondii: An iTRAQ-Based Proteomic Analysis.TOXINS,9(2).
MLA	Chen, Yong,et al."Biodegradation Mechanisms of Patulin in Candida guilliermondii: An iTRAQ-Based Proteomic Analysis".TOXINS 9.2(2017).

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