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Deletion of CGLD1 Impairs PSII and Increases Singlet Oxygen Tolerance of Green Alga Chlamydomonas reinhardtii
Xing, Jiale1; Liu, Peng1; Zhao, Lei; Huang, Fang
2017
发表期刊FRONTIERS IN PLANT SCIENCE
ISSN1664-462X
卷号8
摘要The green alga Chlamydomonas reinhardtii is a key model organism for studying photosynthesis and oxidative stress in unicellular eukaryotes. Using a forward genetics approach, we have identified and characterized a mutant x32, which lacks a predicted protein named CGLD1 (Conserved in Green Lineage and Diatom 1) in GreenCut2, under normal and stress conditions. We show that loss of CGLD1 resulted in minimal photoautotrophic growth and PSII activity in the organism. We observed reduced amount of PSII complex and core subunits in the x32 mutant based on blue-native (BN)/PAGE and immunoblot analysis. Moreover, x32 exhibited increased sensitivity to high-light stress and altered tolerance to different reactive oxygenic species (ROS) stress treatments, i.e., decreased resistance to H2O2/or tert-Butyl hydroperoxide (t-BOOH) and increased tolerance to neutral red (NR) and rose bengal (RB) that induce the formation of singlet oxygen, respectively. Further analysis via quantitative real-time PCR (qRT-PCR) indicated that the increased singlet-oxygen tolerance of x32 was largely correlated with up-regulated gene expression of glutathione-S-transferases (GST). The phenotypical and physiological implications revealed from our experiments highlight the important roles of CGLD1 in maintaining structure and function of PSII as well as in protection of Chlamydomonas under photo-oxidative stress conditions.
关键词C. reinhardtii x32 mutant PSII photo-oxidative stress singlet oxygen
学科领域Plant Sciences
DOI10.1111/jse.12265
收录类别SCI
语种英语
WOS关键词PHOTOSYSTEM-II ; OXIDATIVE STRESS ; HIGH-LIGHT ; THYLAKOID MEMBRANE ; SYNTHETIC BIOLOGY ; SULFUR-DEPLETION ; GENE-EXPRESSION ; PROTEIN ; REPAIR ; CYANOBACTERIA
WOS记录号WOS:000406115000013
出版者FRONTIERS MEDIA SA
文献子类Article
出版地LAUSANNE
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31670239, 31470340] ; National Basic Research Program of China (973 Program)National Basic Research Program of China [2015CB150100] ; Chinese Academy of SciencesChinese Academy of Sciences [XDB17000000, KGCX2-YW-373]
作者邮箱fhuang@ibcas.ac.cn
作品OA属性Bronze
引用统计
被引频次:54[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/22360
专题中科院光生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Xing, Jiale,Liu, Peng,Zhao, Lei,et al. Deletion of CGLD1 Impairs PSII and Increases Singlet Oxygen Tolerance of Green Alga Chlamydomonas reinhardtii[J]. FRONTIERS IN PLANT SCIENCE,2017,8.
APA Xing, Jiale,Liu, Peng,Zhao, Lei,&Huang, Fang.(2017).Deletion of CGLD1 Impairs PSII and Increases Singlet Oxygen Tolerance of Green Alga Chlamydomonas reinhardtii.FRONTIERS IN PLANT SCIENCE,8.
MLA Xing, Jiale,et al."Deletion of CGLD1 Impairs PSII and Increases Singlet Oxygen Tolerance of Green Alga Chlamydomonas reinhardtii".FRONTIERS IN PLANT SCIENCE 8(2017).
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