Histone Acetylation Modifications Affect Tissue-Dependent Expression of Poplar Homologs of C-4 Photosynthetic Enzyme Genes

doi:10.1111/gcbb.12413

IB-CAS > 中科院光生物学重点实验室

	Histone Acetylation Modifications Affect Tissue-Dependent Expression of Poplar Homologs of C-4 Photosynthetic Enzyme Genes
	Li, Yuan 1; Dong, Xiu-Mei ; Jin, Feng ; Shen, Zhuo ; Chao, Qing ; Wang, Bai-Chen
	2017
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	8
摘要	Histone modifications play important roles in regulating the expression of C-4 photosynthetic genes. Given that all enzymes required for the C-4 photosynthesis pathway are present in C-3 plants, it has been hypothesized that this expression regulatory mechanism has been conserved. However, the relationship between histone modification and the expression of homologs of C-4 photosynthetic enzyme genes has not been well determined in C-3 plants. In the present study, we cloned nine hybrid poplar (Populus simonii x Populus nigra) homologs of maize (Zea mays) C-4 photosynthetic enzyme genes, carbonic anhydrase (CA), pyruvate orthophosphate dikinase (PPDK), phosphoenolpyruvate carboxykinase (PCK), and phosphoenolpyruvate carboxylase (PEPC), and investigated the correlation between the expression levels of these genes and the levels of promoter histone acetylation modifications in four vegetative tissues. We found that poplar homologs of C-4 homologous genes had tissue-dependent expression patterns that were mostly well-correlated with the level of histone acetylation modification (H3K9ac and H4K5ac) determined by chromatin immunoprecipitation assays. Treatment with the histone deacetylase inhibitor trichostatin A further confirmed the role of histone acetylation in the regulation of the nine target genes. Collectively, these results suggest that both H3K9ac and H4K5ac positively regulate the tissuedependent expression pattern of the PsnCAs, PsnPPDKs, PsnPCKs, and PsnPEPCs genes and that this regulatory mechanism seems to be conserved among the C-3 and C-4 species. Our findings provide new insight that will aid efforts to modify the expression pattern of these homologs of C-4 genes to engineer C-4 plants from C3 plants.
关键词	poplar C-4 genes homologs transcriptional regulation histone acetylation modification
学科领域	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
DOI	10.1111/gcbb.12413
收录类别	SCI
语种	英语
WOS关键词	ORTHOPHOSPHATE DIKINASE GENE ; PHOSPHOENOLPYRUVATE CARBOXYKINASE ; CARBONIC-ANHYDRASE ; C4 GENES ; PLANT ; ARABIDOPSIS ; EVOLUTION ; LEAVES ; CARBOXYLASE ; PYRUVATE
WOS记录号	WOS:000403225800005
出版者	FRONTIERS MEDIA SA
文献子类	Article
出版地	LAUSANNE
资助机构	National High-Tech Research and Development Program of ChinaNational High Technology Research and Development Program of China [2013AA102701] ; State Key Program of National Natural Science of ChinaNational Natural Science Foundation of China (NSFC) [31030017]
作者邮箱	wangbc@ibcas.ac.cn
作品OA属性	gold
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22361
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Photosynth Res Ctr, Key Lab Photobiol, Inst Bot, Beijing, Peoples R China 2.Northeast Forestry Univ, State Key Lab Forest Genet & Tree Breeding, Harbin, Peoples R China
推荐引用方式 GB/T 7714	Li, Yuan,Dong, Xiu-Mei,Jin, Feng,et al. Histone Acetylation Modifications Affect Tissue-Dependent Expression of Poplar Homologs of C-4 Photosynthetic Enzyme Genes[J]. FRONTIERS IN PLANT SCIENCE,2017,8.
APA	Li, Yuan,Dong, Xiu-Mei,Jin, Feng,Shen, Zhuo,Chao, Qing,&Wang, Bai-Chen.(2017).Histone Acetylation Modifications Affect Tissue-Dependent Expression of Poplar Homologs of C-4 Photosynthetic Enzyme Genes.FRONTIERS IN PLANT SCIENCE,8.
MLA	Li, Yuan,et al."Histone Acetylation Modifications Affect Tissue-Dependent Expression of Poplar Homologs of C-4 Photosynthetic Enzyme Genes".FRONTIERS IN PLANT SCIENCE 8(2017).

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