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| MORF9 increases the RNA-binding activity of PLS-type pentatricopeptide repeat protein in plastid RNA editing | |
Yan, Junjie1; Zhang, Qunxia1; Guan, Zeyuan1; Wang, Qiang1; Li, Li1; Ruan, Fengying1; Lin, Rongcheng2 ; Zou, Tingting1; Yin, Ping1
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| 2017 | |
| 发表期刊 | NATURE PLANTS
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| ISSN | 2055-026X |
| 卷号 | 3期号:5 |
| 摘要 | RNA editing is a post-transcriptional process that modifies the genetic information on RNA molecules. In flowering plants, RNA editing usually alters cytidine to uridine in plastids and mitochondria. The PLS-type pentatricopeptide repeat (PPR) protein and the multiple organellar RNA editing factor (MORF, also known as RNA editing factor interacting protein (RIP)) are two types of key trans-acting factors involved in this process. However, how they cooperate with one another remains unclear. Here, we have characterized the interactions between a designer PLS-type PPR protein (PLS)(3)PPR and MORF9, and found that RNA-binding activity of (PLS)(3)PPR is drastically increased on MORF9 binding. We also determined the crystal structures of (PLS)(3)PPR, MORF9 and the (PLS)(3)PPR-MORF9 complex. MORF9 binding induces significant compressed conformational changes of (PLS)(3)PPR, revealing the molecular mechanisms by which MORF9-bound (PLS)(3)PPR has increased RNA-binding activity. Similarly, increased RNA-binding activity is observed for the natural PLS-type PPR protein, LPA66, in the presence of MORF9. These findings significantly expand our understanding of MORF function in plant organellar RNA editing. |
| 学科领域 | Plant Sciences ; Evolutionary Biology |
| DOI | 10.1600/036364417X696032 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | SINGLE-STRANDED RNA ; STRUCTURAL BASIS ; ARABIDOPSIS-THALIANA ; DYW DOMAIN ; RECOGNITION ; PLANTS ; MAIZE ; MITOCHONDRIA ; BIOGENESIS ; SOFTWARE |
| WOS记录号 | WOS:000409055400006 |
| 出版者 | NATURE PUBLISHING GROUP |
| 文献子类 | Article |
| 出版地 | LONDON |
| EISSN | 2055-0278 |
| 资助机构 | Ministry of Science and Technology [2015CB910900] ; Fok Ying-Tong Education FoundationFok Ying Tung Education Foundation [151021] ; National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [91535301] ; Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [2014PY026, 2015PY219, 2014JQ001] ; Huazhong Agricultural University Scientific & Technological Self-innovation Foundation [2013RC013] ; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2015M572163] |
| 作者邮箱 | yinping@mail.hzau.edu.cn |
| 作品OA属性 | Green Published |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/22362 |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China 2.Huazhong Agr Univ, Natl Ctr Plant Gene Res, Wuhan 430070, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, Junjie,Zhang, Qunxia,Guan, Zeyuan,et al. MORF9 increases the RNA-binding activity of PLS-type pentatricopeptide repeat protein in plastid RNA editing[J]. NATURE PLANTS,2017,3(5). |
| APA | Yan, Junjie.,Zhang, Qunxia.,Guan, Zeyuan.,Wang, Qiang.,Li, Li.,...&Yin, Ping.(2017).MORF9 increases the RNA-binding activity of PLS-type pentatricopeptide repeat protein in plastid RNA editing.NATURE PLANTS,3(5). |
| MLA | Yan, Junjie,et al."MORF9 increases the RNA-binding activity of PLS-type pentatricopeptide repeat protein in plastid RNA editing".NATURE PLANTS 3.5(2017). |
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