Proteomic analysis of Potentilla fruticosa L. leaves by iTRAQ reveals responses to heat stress

doi:10.3389/fpls.2017.01148

	Proteomic analysis of Potentilla fruticosa L. leaves by iTRAQ reveals responses to heat stress
	Guo, Yingtian ; Wang, Zhi 1; Guan, Xuelian ; Hu, Zenghui ; Zhang, Ze ; Zheng, Jian 2; Lu, Yizeng 3
	2017
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	12 期号:8
摘要	High temperature is an important environmental factor that affects plant growth and crop yield. Potentilla fruticosa L. has a developed root system and characteristics of resistance to several stresses (e.g., high temperature, cold, drought) that are shared by native shrubs in the north and west of China. To investigate thermotolerance mechanisms in P. fruticosa, 3-year-old plants were subjected to a high temperature of 42 degrees C for 1, 2, and 3 days respectively before analysis. Then, we studied changes in cell ultrastructure using electron microscopy and investigated physiological changes in the leaves of P. fruticosa. Additionally, we used isobaric tags for relative and absolute quantification (iTRAQ) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study proteomic changes in P. fruticosa leaves after 3 d of 42 degrees C heat stress. we found that the cell membrane and structure of chloroplasts, especially the thylakoids in P. fruticosa leaves, was destroyed by a high temperature stress, which might affect the photosynthesis in this species. We identified 35 up-regulated and 23 down-regulated proteins after the heat treatment. Gene Ontology (GO) analysis indicated that these 58 differentially abundant proteins were involved mainly in protein synthesis, protein folding and degradation, abiotic stress defense, photosynthesis, RNA process, signal transduction, and other functions. The 58 proteins fell into different categories based on their subcellular localization mainly in the chloroplast envelope, cytoplasm, nucleus, cytosol, chloroplast, mitochondrion and cell membrane. Five proteins were selected for analysis at the mRNA level; this analysis showed that gene transcription levels were not completely consistent with protein abundance. These results provide valuable information for Potentilla thermotolerance breeding.
学科领域	Plant Sciences
DOI	10.3389/fpls.2017.01148
收录类别	SCI
语种	英语
WOS关键词	ENVELOPE MEMBRANE POLYPEPTIDE ; NUCLEOTIDE-EXCHANGE FACTOR ; ARABIDOPSIS-THALIANA ; PEROXIREDOXIN-Q ; CHLOROPHYLL BREAKDOWN ; OXIDATIVE STRESS ; SHOCK PROTEINS ; EXPRESSION ; TOLERANCE ; RESISTANCE
WOS记录号	WOS:000404602800001
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
资助机构	Importation and Development of High-Caliber Talents Project of Beijing Municipal Institutions [CITTCD20154043] ; Beijing Municipality [CITIDHT20150503] ; Beijing Laboratory of Urban and Rural Ecological Environment [PXM2015-014207000014]
作者邮箱	buazhenglian708@126.com
作品OA属性	gold, Green Published
引用统计	被引频次：88[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22365
专题	中科院植物分子生理学重点实验室
作者单位	1.Beijing Agr Univ, Coll Landscape Architecture, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 3.Beijing Collaborat Innovat Ctr Ecoenvironm Improv, Beijing, Peoples R China 4.Shandong Forest Germplasm Resources Ctr, Jinan, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Guo, Yingtian,Wang, Zhi,Guan, Xuelian,et al. Proteomic analysis of Potentilla fruticosa L. leaves by iTRAQ reveals responses to heat stress[J]. PLOS ONE,2017,12(8).
APA	Guo, Yingtian.,Wang, Zhi.,Guan, Xuelian.,Hu, Zenghui.,Zhang, Ze.,...&Lu, Yizeng.(2017).Proteomic analysis of Potentilla fruticosa L. leaves by iTRAQ reveals responses to heat stress.PLOS ONE,12(8).
MLA	Guo, Yingtian,et al."Proteomic analysis of Potentilla fruticosa L. leaves by iTRAQ reveals responses to heat stress".PLOS ONE 12.8(2017).

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